Determination of Starch Lysophospholipids in Rice Using Liquid Chromatography–Mass Spectrometry (LC-MS)

Abstract

1Acquisition of a complete understanding of rice starch lysophospholipids (LPLs), 2 their biosynthetic pathways, genetic diversity, and the influence of genotype by 3 environment interactions has been hampered by the lack of efficient high throughput 4 extraction and analysis methods. We hypothesized a single-step aqueous n-propanol 5 extraction combined with liquid chromatography mass spectrometry (LCMS) could be 6 employed to analyze starch LPLs in white rice. Our investigation found different grinding 7 methods showed little effect on the final LPL detected and a simple single-step extraction 8 with 75% n-propanol (8mL/0.15g) heated at 100 o

Rapid tin-mediated access to a lysophosphatidylethanolamine (LPE) library: Application to positional LC/MS analysis for hepatic LPEs in non-alcoholic steatohepatitis model mice

“…C18:1 (10) and C18:2 (11) LPEs appear transitional; C18:1 (10) is 2-LPE rich whereas C18:2 (11) is 1-LPE rich. This remarkable difference can't be explained by the theory of chemical equilibrium: Thermodynamically more stable 2-LPE (1-Acyl) should be favorable than 1-LPE (2-Acyl) as reported in previous studies (Plückthun and Dennis, 1982;Liu, et al, 2014). Thus, a contribution of biological or enzymatic factors is anticipated: for example, the different substrate specificity of lipases in a liver tissue, such as LPA1 or LPA2 (Okudaira, et al, 2014), but this hypothesis needs to be proven in future.…”

“…Another frequently encountered problem in LPE and LPL research is 1,2-O-acyl migration ( Figure 1) (Plückthun and Dennis, 1982;Liu, et al, 2014), as this equilibrium furnishes 1-LPE and 2-LPE regioisomers, which exhibit different physico-chemical (Okudaira, et al, 2014) and biological (Xu, et al, 2005) properties. Unfortunately, suppression of this migration still remains difficult, and accordingly the precise determination of the regioisomer ratio prior to a use of the mixture is of paramount importance.…”

Characterisation of cannabinoid composition in a diverse Cannabis sativa L. germplasm collection

“…Flow rate and injection volume were set at 0.3 mL/minute and 3 lL respectively. MSD parameters and settings followed those of a previously validated method (Liu et al 2014). The MSD was operated in atmospheric pressure ionization-electrospray mode using the following parameters: scan mass range, 100-1200; fragmentor, 150; capillary voltage, 3000 V (positive); drying gas flow, 12.0 L/min (N 2 ); vaporizer temperature, 350°C; nebuliser pressure, 35 psi; drying gas temperature, 350°C.…”

The contribution of lysophospholipids to pasting and thermal properties of nonwaxy rice starch

“…The rice endosperm lysophospholipids were determined according to Liu et al (2014) and all data from the two sets of rice accessions were previously reported Tong, Liu, et al, 2014).…”