Determination of Steroidal Saponins in Tribulus terrestris by Reversed-Phase High-Performance Liquid Chromatography and Evaporative Light Scattering Detection

Ganzera, Markus; Bedir, Erdal; Khan, Ikhlas A.

doi:10.1002/jps.1124

Cited by 111 publications

(104 citation statements)

References 7 publications

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“…The contents of saponins in the fraction selected for the experiments were determined to be more than 99% by photometric analysis described previously [14]. In addition, the fraction was standardized on base of protodioscin contents by RP-HPLC [15] using a commercially available standard (ChromaDex, Inc., Santa Ana, CA). The used fraction was found to contain no less than 94 % protodioscin.…”

Section: Methodsmentioning

confidence: 99%

Changes in gene expression of CXCR4, CCR7 and BCL2 after treatment of breast cancer cells with saponin extract from Tribulus terrestris

Goranova¹,

Ss²,

Vs³

et al. 2015

neo

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Saponins are natural substances produced by a large number of plants, one of which is Tribulus terrestris L. (TT). They have been reported to possess an antitumor activity exerted by regulating various signaling pathways in the cell. Although the mechanisms of action of saponin extracts from various plants have been widely studied, limited data are available about TT. The present study aimed to analyze the impact of saponin extract from TT on cell processes in breast carcinoma cell lines. The variations in expression of a group of 32 selected genes were examined by real-time PCR after saponin treatment of MCF7 and MCF10A cell lines. Only three genes -CXCR4, CCR7 and BCL2, showed changes in their mRNA levels after the application of the herb extract. While CXCR4 expression was reduced in both cell lines, CCR7 and BCL2 levels decreased only in tumorigenic MCF7 cells, implying cell-specificity of the saponin action. Our results suggested that TT extract containing saponins was likely to affect the processes of apoptosis and metastasizing of cancer cells. Further in vivo studies will show its applicability as an anticancer therapeutic agent.

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Section: Methodsmentioning

confidence: 99%

Changes in gene expression of CXCR4, CCR7 and BCL2 after treatment of breast cancer cells with saponin extract from Tribulus terrestris

Goranova¹,

Ss²,

Vs³

et al. 2015

neo

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“…Chromatographic and Sophisticated modern techniques of standardization such as spectroscopic evaluation UV-vis spectrophotometry, TLC, HPTLC [86,87], HPLC [88][89][90][91], NMR [92,93], near infrared spectroscopy [94] provide quantitative and semiquantitative information about the main active constituents or marker compounds present in the crude drug or herbal products.…”

Section: Physical Parametersmentioning

confidence: 99%

Protocols on safety, efficacy, standardization, and documentation of herbal medicine (IUPAC Technical Report)

Mosihuzzaman

Choudhary

2008

Pure and Applied Chemistry

112

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This Technical Report compiles and analyzes the current scientific knowledge on herbal medicine and highlights the practical ways for ensuring the safety of herbal preparations and evaluating their claimed efficacy. Emphasis has been given to the methods for standardization of herbal medicine and the ways and means for moving forward to achieve the difficult goal of preparing herbal medicines of consistent quality and effects. Pragmatic approaches have been recommended to overcome the difficulties in (i) protecting intellectual property rights (IPR); (ii) producing safe, potent, standardized, and affordable herbal medicine; and (iii) documenting the knowledge base on herbal medicine in an easily accessible format.

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“…Publications on TT have reported the isolation of several steroid saponins of furostanol type [15][16][17], with protodioscin as the most dominant one [12], as well as alkaloids and flavonoids [18][19][20][21][22], and recently new feruloyl amide derivative named as tribulusamide C [23]. Obreshova et al [15] identified protogracilin besides protodioscin as the dominate furostanol bisglycoside and, so these compounds are considered to be factor responsible for biological activity of products derived from this plant [14].…”

Section: Introductionmentioning

confidence: 99%

“…Besides Tribulus, it has been identified in a number of plant species such as Trigonella and Dioscorea families [16,24,25]. Thus, protodioscin has been isolated as ethanol extract from rhizomes of Dioscorea panthaica L. [25] and purified and identified from Trigonella foenumgraecum L. [24].…”

Section: Introductionmentioning

confidence: 99%

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A Simple Method for Determination of Protodioscin in Tribulus Terrestris L. and Pharmaceuticals by High-Performance Liquid Chromatography Using Diode-Array Detection

Shishovska

Arsova‐Sarafinovska

Memeti

2015

J. Chem. Eng. Res. Updates.

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Abstract:The Tribulus terrestris L. (Zygophyllaceae) is a known medical plant used in traditional and folk medicine worldwide. The steroid saponine protodioscin, an active component found in this plant, serves as a marker for quality control of plant raw materials. In this study, we developed a simple and selective method for determination of protodioscin using convenient High-Performance Liquid Chromatographic (HPLC) laboratory equipment. The detection was performed by Diode-Array Detector (DAD). The proposed method was fully validated and according to the validation results, it was accurate and precise. It was proven to be linear over a protodioscin concentration range of 10,9 to 544.9 µg/mL. The low values of limit of detection (LOD) and limit of quantitation (LOQ) demonstrated adequate sensitivity (16.0 µg and 48.6 µg protodioscin per g plant material, respectively). The proposed method was successfully applied for quality control of a raw plant material intended for use in pharmaceutical industry, as well as for determination of protodioscin in a commercially available pharmaceutical formulation. The positive identification of protodioscin in analyzed samples was done by comparison of retention times of chromatographic peaks and their UV spectra. The content of protodioscin in analyzed samples was: 0.65 -0.73 % in a raw plant material, and 0.38 % in tablets, respectively.

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Determination of Steroidal Saponins in Tribulus terrestris by Reversed-Phase High-Performance Liquid Chromatography and Evaporative Light Scattering Detection

Cited by 111 publications

References 7 publications

Changes in gene expression of CXCR4, CCR7 and BCL2 after treatment of breast cancer cells with saponin extract from Tribulus terrestris

Changes in gene expression of CXCR4, CCR7 and BCL2 after treatment of breast cancer cells with saponin extract from Tribulus terrestris

Protocols on safety, efficacy, standardization, and documentation of herbal medicine (IUPAC Technical Report)

A Simple Method for Determination of Protodioscin in Tribulus Terrestris L. and Pharmaceuticals by High-Performance Liquid Chromatography Using Diode-Array Detection

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