Determination of streptomycin in eggs yolk by capillary electrophoresis

Abstract

KeyWords Capillary electrophoresis Streptomycin Eggs yolk Maximum residue limits SummaryA capillary electrophoresis method for the determination of streptomycin in eggs is described. Analyses were performed on an uncoated silica capillary using a buffer solution of 30mM sodium dihydrophosphate, 5 mM boric acid and 5 mM sodium tetraborate. Analytes were detected at 200 nm an the calibration curve was linear over the range of0.16 to 2.0/~g g 1 (r = 0.999). The total analysis time was 7 min. The method has been successfully applied to the quantitative determination of streptomycin in hen eggs after drug ingestion and could used for evaluation of maximum residue limits.

Analysis of antibiotics by capillary electrophoresis

“…Selectivity obtained with CE was similar to that of an HPLC method, but with a much shorter analysis time than the HPLC method [3]. Another method was used to determine residual streptomycin in egg yolks [4].…”

Simultaneous determination of streptomycin and oxytetracycline in agricultural antimicrobials by CZE after an experimental design

“…The running electrolytes recommended in the literature for the determination of OTC by CZE are sodium phosphate (pH 2.0) or sodium carbonate (pH 11.2) [25,26] and for STP are sodium tetraborate (pH 9.0 or 10.25) or sodium dihydrophosphate, boric acid and sodium tetraborate (pH 6.35) [9,11,22]. Thus, in order to optimize the simultaneous determination of STP and OTC different compositions of background electrolyte were evaluated: sodium phosphate (pH 2.0 and 8.5), sodium carbonate (pH 8.5 and 10) and sodium tetraborate (pH 9.0).…”

“…Several studies using capillary electrophoresis for the determination of streptomycin [9,11,22,23,24] or oxytetracycline [4,25,26,27,28,29] in a variety of matrices have been reported, but not for both simultaneously. Hsiao et al [30] proposed a CZE method for the simultaneous determination of STP and OTC, however these authors did not offer a detailed study about the influence of the composition of the electrolyte on the separation of the compounds or indicate the ruggedness of the method.…”

“…The determination of aminoglycosides typically requires the utilization of microbiological techniques that are more qualitative than quantitative [8][9][10][11]. Chemical methods of analysis have included high performance liquid chromatography (HPLC) with either pre-column [12] or post-column derivatization [13], HPLC with electrochemical detection [14], ion pair chromatography [15], agarose gel electrophoresis [16], immunoassay [17] and mass spectrometry [18,19].…”

Determination of organic contaminants in food by capillary electrophoresis

“…Under the right temperature and moisture conditions, stored food products contaminated by various microorganisms may become toxic. Certain types of microorganism may also proliferate on entering in gastrointestinal tract, where the conditions contribute to their development [22,23].…”