Determination of sucrose in equine serum using liquid chromatography–mass spectrometry (LC/MS)

D'Arcy-Moskwa, Eliza; Weston, Leslie A.; Noble, Glenys; Raidal, Sharanne

doi:10.1016/j.jchromb.2011.09.049

Cited by 8 publications

(10 citation statements)

References 17 publications

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“…Previous reports also used a mixture of acetonitrile: water for precipitation of plasma proteins in the assay of natural sucrose in the serum of bottlenose dolphins [20] and horses [18].…”

Section: Discussionmentioning

confidence: 99%

“…We propose that the advancement of new analytical methods, such as LC-MS/MS, should enable the use of non-radioactive sucrose as marker of BBB permeability. In fact, tandem mass spectrometry methods for measurement in body fluids of various sugars, including sucrose, have recently been described [18][19][20]. It is obvious, however, that sucrose from dietary sources is present in plasma of humans and animals at variable concentrations in the low micromolar range [18,21].…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of a sensitive UPLC–MS/MS method for the quantitation of [ 13 C]sucrose in rat plasma, blood, and brain: Its application to the measurement of blood-brain barrier permeability

Miah

Bickel

Mehvar

2016

Journal of Chromatography B

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Accurate and reproducible measurement of blood-brain barrier (BBB) integrity is critical in the assessment of the pathophysiology of the central nervous system disorders and in monitoring therapeutic effects. The widely-used low molecular weight marker [(14)C]sucrose is non-specific in the absence of chromatographic separation. The purpose of this study was to develop and validate a sensitive and reproducible LC-MS/MS method for the analysis of stable isotope-modified [(13)C12]sucrose in brain, plasma, and blood to determine BBB permeability to sucrose. After addition of internal standard (IS, [(13)C6]sucrose), the marker and IS were recovered from diluted rat blood, plasma, and brain homogenate by protein precipitation using acetonitrile. The recovery of the marker and IS was almost quantitative (90-106%) for all three matrices. The recovered samples were directly injected into an isocratic UPLC system with a run time of 6 min. Mass spectrometry was conducted using multiple reaction monitoring in negative mode. The method was linear (r(2)≥0.99) in the concentration ranges tested for the diluted blood and plasma (10-1000 ng/mL) and brain homogenate (1-200 ng/mL). The lower limit of quantitation of the assay was 0.5 pg injected on column. The assay was validated (n=5) based on acceptable intra- and inter-run accuracy and precision values. The method was successfully used for the measurement of serial blood and plasma and terminal brain concentrations of [(13)C12]sucrose after a single intravenous dose (10 mg/kg) of the marker to rats. As expected, the apparent brain uptake clearance values of [(13)C12]sucrose were low in healthy rats. The method may be useful for determination of the BBB integrity in animal models.

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“…Previous reports also used a mixture of acetonitrile: water for precipitation of plasma proteins in the assay of natural sucrose in the serum of bottlenose dolphins [20] and horses [18].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and validation of a sensitive UPLC–MS/MS method for the quantitation of [ 13 C]sucrose in rat plasma, blood, and brain: Its application to the measurement of blood-brain barrier permeability

Miah

Bickel

Mehvar

2016

Journal of Chromatography B

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“…Gastric permeability was assessed for each horse on Day 0 and Day 13, before and after 14 days of NSAID or placebo administration, by determination of sucrose absorption as described previously. 9 Horses were fasted overnight (a minimum of 12 hours) before gastroscopy and then received 0.5 g/kg body weight of sucrose as a 10% solution in tap water via nasogastric intubation. Blood samples were collected from the jugular vein at 0 (before), 15, 30, 45, 60, 90, 120, and 240 minutes post sucrose administration from an aseptically placed intravenous catheter.…”

Section: Permeability Test Proceduresmentioning

confidence: 99%

“…9 Assay validation, specificity, and sensitivity have been described. 9 The limit of detection was 50 pg/lL, although concentrations as low as 11 pg/lL were detected. Study samples were run in duplicate, firstly by treatment group and then in randomized order to exclude potential confounding of results caused by sample handling.…”

Section: Liquid Chromatography/mass Spectrometrymentioning

confidence: 99%

“…Sucrose permeability testing has been explored as an alternative diagnostic tool to gastroscopy in horses, and has been utilized to investigate effects of NSAID administration in other species . A liquid chromatography/mass spectrometry technique was developed and validated for the quantitation of sucrose in equine serum to compare the effect of meloxicam and PBZ treatments on gastric mucosal permeability. Findings were compared with endoscopic gastric ulceration scores and plasma protein concentrations determined in the broader context of a wider study on the safety of PO meloxicam in horses…”

Section: Introductionmentioning

confidence: 99%

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Effects of Meloxicam and Phenylbutazone on Equine Gastric Mucosal Permeability

D'Arcy-Moskwa

Noble

Weston

et al. 2012

Veterinary Internal Medicne

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Background: Newer NSAIDs that more selectively target the induced isoform of the cyclooxygenase enzyme (COX2) activity might reduce adverse effects while preserving therapeutic benefits of these drugs.Objectives: To compare the effect of oral administration of multiple dose rates of meloxicam and phenylbutazone (PBZ) on gastric mucosal integrity in horses.Animals: Twenty-five light breed horses. Methods: In vivo toxicity study. Horses were randomly assigned to 5 treatment groups, receiving placebo, PBZ (4.4 mg/kg PO q12h day 1, 2.2 mg/kg PO q12h for 4 days, 2.2 mg/kg PO q24h for 9 days), or 3 dose rates of meloxicam (0.6 mg/kg q24h, 1.8 mg/kg q24h, 3.0 mg/kg q24h) for 14 days. Sucrose permeability testing was performed on Day 0 (before treatment) and on Day 13. All personnel involved with data collection or analysis were blinded to treatment.Results: Administration of PBZ at the above dose rate significantly increased gastric permeability to sucrose, evidenced by increased peak serum sucrose concentrations (280-1,580 pg/lL, P = .001) after treatment. Similar changes were not evident after administration of meloxicam at any dose rate tested, or in control horses (P > .05). Treatment was not associated with significant differences in ulceration of the squamous or glandular mucosa. Peak sucrose concentrations were not correlated with serum total protein or albumin concentrations (R 2 = À0.07, P = .61, R 2 = À0.08, P = .58, respectively). Conclusion and Clinical Importance: These results suggest that PBZ was associated with greater compromise to gastric mucosal integrity than meloxicam.

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An evaluation of sucrose as a possible contaminant in e-liquids for electronic cigarettes by hydrophilic interaction liquid chromatography–tandem mass spectrometry

et al. 2014

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The influence of sucrose combustion products on smoking and nicotine addiction is still controversial because the presence of the sucrose may be treated as a source of aldehydes and organic acids. In e-liquids used as refills for electronic cigarettes, which are made primarily of poly(propylene glycol), glycerine and ethanol, sucrose may be present at trace levels, and its impact on mainstream smoke formation, and hence on human health and smoking/nicotine addiction is unknown. An analytical method was developed where high-performance liquid chromatography in hydrophilic interaction liquid chromatography mode and tandem mass spectrometry were used for fast and simple determination of sucrose and other saccharides in e-liquids for electronic cigarettes. Minimal effort was required in the sample preparation step, and satisfactory results were obtained, and the sample matrix had an insignificant impact. The chromatographic separation was done using an Ascentis Express OH5 column (150 mm × 2.1 mm, 2.7 μm). The coefficients of variation for within-day precision for three concentrations were 2.4 %, 1.6 % and 2.3 %, and the between-day coefficients of variation for a single concentration were 2.1 %, 2.5 % and 1.7 % measured on the next 3 days. The detection limit was 0.73 μg/g, and the sucrose content in e-liquids ranged from 0.76 to 72.93 μg/g among 37 samples. Moreover, with the method presented it is possible to determine the presence of other saccharides such as fructose, glucose, maltose and lactose. However, only sucrose was found in all samples of e-liquids. The proposed method is rapid, simple and reliable in terms of high-performance liquid chromatography coupled with tandem mass spectrometry.

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Determination of sucrose in equine serum using liquid chromatography–mass spectrometry (LC/MS)

Cited by 8 publications

References 17 publications

Development and validation of a sensitive UPLC–MS/MS method for the quantitation of [ 13 C]sucrose in rat plasma, blood, and brain: Its application to the measurement of blood-brain barrier permeability

Development and validation of a sensitive UPLC–MS/MS method for the quantitation of [ 13 C]sucrose in rat plasma, blood, and brain: Its application to the measurement of blood-brain barrier permeability

Effects of Meloxicam and Phenylbutazone on Equine Gastric Mucosal Permeability

An evaluation of sucrose as a possible contaminant in e-liquids for electronic cigarettes by hydrophilic interaction liquid chromatography–tandem mass spectrometry

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