Determination of sugars and alditols in tobacco with high performance anion‐exchange chromatography

Abstract

Sugars, alditols, and alcohols in tobacco products were quantified utilizing a method of high-performance anion-exchange chromatography-pulsed amperometric detection (HPAEC-PAD). The optimized analytical method can be used to classify different tobaccos and control the quality of tobaccos. To substantiate the applicability of the method, the analysis of 27 tobacco samples with satisfactory linearity, repeatability, and accuracy had been demonstrated. Compared with some other analytical methods for tobacco, the HPAEC-PAD method provided a simple and powerful tool for the analysis of not only sugars but also alcohols in tobacco extracts.

Determination of carbohydrates in tobacco by pressurized liquid extraction combined with a novel ultrasound-assisted dispersive liquid–liquid microextraction method

“…Traditionally, solvent extraction and solid-phase extraction purification are the most widely used treatment methods for the analysis of the main carbohydrates in tobacco [3][4][5][6][7][8][9][10]. However, drawbacks such as the need for multiple extractions and the large required volume of toxic organic solvent make solvent extraction expensive, time-consuming, and environmentally unfriendly.…”

“…The analysis of the main carbohydrates in tobacco and tobacco products is typically accomplished through the use of segmented flow analysis (SFA) [3], gas chromatography (GC) with derivatization [4], high-performance liquid chromatography (HPLC) [5][6][7][8], and ion chromatography (IC) [9][10]. Compared with the other methods, GC with derivatization shows some unique advantages.…”

An isotope dilution ultra high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of sugars and humectants in tobacco products

“…Such differentiation is important because federal tax rates in the United States differ across tobacco product categories and sub-categories, and product characteristics could impact regulatory efforts. Moreover, sugar levels are known to differ among certain product types [9]. …”

“…Existing methods for humectant detection range from IC to GC to GC-MS [2, 10]. Tang et al developed an IC method, and applied it to analyze 12 sugars, alditols, and humectants in tobacco [9]. Rainey, et al concluded that a GC-MS method can compensate for the inadequate chromatographic resolution between the analyzed humectants [2].…”

“…Sample run time also shortened to 15 min from 20 min [17]. The IC method described by Tang et al needs 55 min to detected 12 sugars and humectants [9]. To demonstrate utility, this method was used to characterize selected samples of three different types of combustible tobacco products: cigarettes, little cigars and cigarillos.…”