Determination of Sulfadiazine and N4-Acetylsulfadiazine in Biological Fluids by Liquid Chromatography on Silica Gel with an Aqueous Buffer as Mobile Phase

Westerlund, Douglas; Wijkström, Agneta

doi:10.1002/jps.2600711016

Cited by 10 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Much of the experimental data which emerged during the development of co-trimazine has not yet been published. It is the purpose of this article to review the key studies on the pharmacokinetics of co-trimazine, both published and unpublished, 1 particularly those that have compared it with co-trimoxazole to show the influence offood, age, renal disease, etc., and those that have assessed the tissue penetration of the component drugs assayed with specific chemical methods (C>rtengren & Treiber 1974;Reeder 1972;Vree et al 1978;Westerlund & Wijkstrom 1982). …”

mentioning

confidence: 99%

Clinical Pharmacokinetics of Co-Trimazine

Bergan¹,

Örtengren²,

Westerlund³

1986

Clinical Pharmacokinetics

Self Cite

View full text Add to dashboard Cite

The clinical pharmacokinetics of co-trimazine (trimethoprim plus sulphadiazine) are reviewed and compared with those of co-trimoxazole (trimethoprim plus sulphamethoxazole). Both combination drugs have similar serum half-life values in persons with normal renal function (half-life of 8 to 12 hours), but the sulphamethoxazole metabolites are retained more than trimethoprim in reduced renal function. Sulphadiazine is less metabolised and the total sulphonamide load of therapeutic doses of co-trimazine is therefore less than for co-trimoxazole. Both co-trimazine and co-trimoxazole have high bioavailability. A suspension of co-trimazine gives serum concentrations comparable with those of tablets. The extravascular penetration of the co-trimazine components is reflected by the total area under the lymph concentration curve in comparison with serum. This measure shows a penetration into peripheral human lymph of 68% for sulphadiazine and 59% for trimethoprim. The proportions eliminated in urine are about 55% for sulphadiazine, 30% for its acetylated metabolite and 75% for trimethoprim. In comparison, for co-trimoxazole, the proportion of sulphamethoxazole eliminated in urine is 15%, that of the acetylated derivative 47%, and that of trimethoprim is also 75%. Urine concentrations of both combinations have similar bioactivity against urinary pathogens after 500 mg of co-trimazine and 960 mg of co-trimoxazole.

show abstract

mentioning

confidence: 99%

Clinical Pharmacokinetics of Co-Trimazine

Bergan¹,

Örtengren²,

Westerlund³

1986

Clinical Pharmacokinetics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Metal complexes of sulfadizine was also exploited as a therapeutic agent, zinc sulfadizine is used to prevent bacterial infection in burned animal 4 . There are several ISSN Print: 2229-7928 ISSN Online: 2230-7532 methods reported for the estimation of sulfadiazine, they include HPLC [5][6][7][8][9] in addition to the UV detector Mass spectroscopic determination was also employed in the determination of sulfadiazine [10][11][12] . Spectrophotometric method was also applied for the estimation of sulfadiazine 1,13 continuous flow spectrophotometric 14 and flow injection method 15 for determination of sulfadiazine is also available in the literature.…”

Section: Introductionmentioning

confidence: 99%

Permanganate Oxidation of Sulfadiazine: Analytical Method Development and Kinetic Study

Siddiqui

ALOthman

Rafiquee

et al. 2013

Analytical Chemistry Letters

View full text Add to dashboard Cite

A multi-disciplinary approach has been made in this article where, the oxidation reaction of permanganate and sulfadiazine has been used to develop analytical method to quantitate the drug and the second part is the kinetic study of the oxidation reaction. The estimation of the drug is made at 602 nm. The developed method has been validated as per the international conference on harmonization (ICH) guidelines. The regression treatment of the linearity data yielded the linear regression equation A = 0.00786 + 0.0184 C. Limit of detection and limit of quantitation was found to be 2.33 and 7.065, respectively. Since, the reaction proceeded such that the absorbance increased with increase in time, kinetic study of the reaction was performed. The kinetic parameters were performed at 602 nm. The reaction obeyed pseudo first-order kinetics in [alkaline KMnO4] and fractional order in [sulfadiazine]. The oxidation reaction was carried out at varied temperatures viz. 25, 30, 35, 40 and 45 (±0.1°C). On the basis of the observed results, mechanism of the reaction has been proposed. Activation parameters such as energy of activation, enthalpy, entropy and Gibbs free energy were determined and found to be 8.73 (kJ mol-1), 77.57 (kJ mol-1), 173 (JK-1 mol-1), 25.99 (kJ mol-1) respectively.

show abstract

“…8 The United States Pharmacopoeia (USP) proposes a chromatographic separation before UV quantification of SDZ. 9 Other methods for SDZ determination are meant for the analysis of food [10][11][12][13][14][15][16][17][18][19][20] or feeding stuffs, 21 biological fluids, [22][23][24][25][26][27] or environmental samples. [28][29][30][31] These concern more complex and high cost procedures, unsuitable for the routine control of formulated pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%