Determination of T-2 and HT-2 Toxins in Cereals Including Oats after Immunoaffinity Cleanup by Liquid Chromatography and Fluorescence Detection

Trebstein, Anke; Seefelder, Walburga; Lauber, U.; Humpf, Hans‐Ulrich

doi:10.1021/jf800316m

Cited by 65 publications

(47 citation statements)

References 15 publications

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“…Production of mycotoxins and analyses DON was produced by Fusarium graminearum (NRRL 5883) and T-2 toxin by Fusarium sporotrichioides (NRRL 3299) strains on corn substrate according to Fodor et al (2006). DON and 15-acetyl DON content of the feed was determined according to Pussemier et al (2006), while T-2 and HT-2 concentration was measured by HPLC after immunoaffinity cleanup (Trebstein et al, 2008).…”

Section: Experimental Designmentioning

confidence: 99%

Short-term effects of T-2 toxin or deoxynivalenol on lipid peroxidation and the glutathione system in common carp

Pelyhe

Kövesi

Zándoki

et al. 2016

Acta Veterinaria Hungarica

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The purpose of this study was to investigate the short-term effects of a single oral dose of T-2 and HT-2 toxin at 0.15, 0.33 and 1.82 mg kg -1 body weight, or deoxynivalenol (DON) and 15-acetyl-DON at 0.13, 0.31 and 1.75 mg kg -1 body weight in common carp. Conjugated dienes and trienes (the early markers of lipid peroxidation) were elevated in all DON-treated groups at the 16th hour, while thiobarbituric acid reactive substances (TBARS; termination marker) were increased at the highest dose of DON at the 16th and 24th hours. T-2 toxin did not cause changes in these parameters. Glutathione content and glutathione peroxidase activity showed higher levels at the 16th hour as the effect of both mycotoxins. The expression of glutathione peroxidase (GPx4) genes (gpx4a and gpx4b) revealed a dual response. Downregulation was observed at the 8th hour, followed by an induction at the 16th hour, at the lowest dose of both mycotoxins. Higher doses revealed long-drawn emergence and an elevation was observed only at the 24th hour. However, at the lowest and highest doses of DON or T-2 toxin the changes in gene expression were delayed, which may be related to the low oxidative stress response, as suggested by the expression profiles of the nrf2, keap1, gpx4a and gpx4b genes.

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Section: Experimental Designmentioning

confidence: 99%

Short-term effects of T-2 toxin or deoxynivalenol on lipid peroxidation and the glutathione system in common carp

Pelyhe

Kövesi

Zándoki

et al. 2016

Acta Veterinaria Hungarica

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“…T-2 toxin content of the diets was determined with HPLC method after immunoaffitiny clean up according to Trebstein et al (2008).…”

Section: Production Of Toxin and Determination Of Mycotoxin Content Imentioning

confidence: 99%

Effects of Long-term Feeding of Graded Levels of T-2 Toxin-contaminated Diets on Performance, Some Lipid Peroxide and Glutathione Redox Status Parameters of Broiler Chickens

et al. 2015

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The effect of two different contamination levels of T-2 toxin (1.5 or 3.4 mg/kg feed) was investigated in a 28-days feeding trial on body weight, relative weight of liver and spleen, and some lipid peroxidation and glutathione redox parameters of 14-days old broiler chicken. The results showed that T-2 toxin decreased significantly the body weight at both contamination levels and showed a dose-dependent tendency. Relative weight of liver increased till the end of the trial, while relative weight of spleen was lower at both samplings at lower level of T-2 toxin exposure. Initial phase of lipid peroxidation (conjugated dienes and trienes) was not detected in the liver, but as product of later phase, thiobarbituric acid reactive substances increased significantly, except in the liver. Glutathione content on day 14 was higher in liver homogenate as compared to the control at the lower T-2 toxin contamination level. On day 28 it was higher in blood plasma at the higher and in liver homogenate at both levels of T-2 contamination. Glutathione peroxidase activity on day 14 was significantly higher in liver and spleen homogenates as compared to the control at the lower level of T-2 toxin contamination. On day 28, significantly higher activity was found at both T-2 toxin contamination levels in liver homogenate, and at the lower contamination level in spleen homogenate as compared to the control.The results revealed that T-2 toxin exposure initiates lipid peroxidation and activates the glutathione redox system as well, but the changes were irrespective of the dose-and partly duration of the exposure.

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“…1-antroylnitrile (1-AN) was provided by Wako (SOBIODA SAS, France). DMAP and 1-AN were prepared in toluene as reported by Trebstein, Seefeldet (16). The HPLC-FLD methodology used in this study was adapted from Lippolis, Pascale (17), Pascale, Haidukowski (18), Trebstein, Seefeldet (16), Trebstein,Marschik (19), and Visconti, Lattanzio (20) to measure the T-2 toxin in liquid cultures.…”

Section: Determination and Quantifi Cation Of T-2 Toxin By Hplc/fldmentioning

confidence: 99%

Control of T-2 Toxin in Fusarium Langsethiae and Geotrichum Candidum Co-Culture / Kontrola Toksina T-2 U Kokulturi Fusarium Langsethiae I Geotrichum Candidum

Gastélum-Martínez

Compant

Taillandier

et al. 2012

Archives of Industrial Hygiene and Toxicology

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Due to contamination of barley grains by Fusarium langsethiae, T-2 toxin can be present in the brewing process. It has been observed that the presence of the yeast Geotrichum candidum during malting can reduce the fi nal concentration of this mycotoxin in beer. In this work, a co-culture method was carried out for both microorganisms in order to evaluate the effect on T-2 mycotoxin concentration in comparison with the pure culture of F. langsethiae in the same conditions. The microbial growth of both microorganisms was assessed using three different methods: dry weight, DOPE-FISH, and DNA quantifi cation. In coculture, both microorganisms globally developed less than in pure cultures but G. candidum showed a better growth than F. langsethiae. The concentration of T-2 was reduced by 93 % compared to the pure culture. Hence, the interaction between G. candidum and F. langsethiae led to a drastic mycotoxin reduction despite the only partial inhibition of fungal growth.

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Determination of T-2 and HT-2 Toxins in Cereals Including Oats after Immunoaffinity Cleanup by Liquid Chromatography and Fluorescence Detection

Cited by 65 publications

References 15 publications

Short-term effects of T-2 toxin or deoxynivalenol on lipid peroxidation and the glutathione system in common carp

Short-term effects of T-2 toxin or deoxynivalenol on lipid peroxidation and the glutathione system in common carp

Effects of Long-term Feeding of Graded Levels of T-2 Toxin-contaminated Diets on Performance, Some Lipid Peroxide and Glutathione Redox Status Parameters of Broiler Chickens

Control of T-2 Toxin in Fusarium Langsethiae and Geotrichum Candidum Co-Culture / Kontrola Toksina T-2 U Kokulturi Fusarium Langsethiae I Geotrichum Candidum

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