Determination of T.N.T. Metabolites in Urine

Almog, Joseph; Kraus, Sarah; Basch, Alvin

doi:10.1007/978-3-642-69083-9_64

Cited by 12 publications

(4 citation statements)

References 3 publications

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“…Mutagenic acttivity and metabolites in the urine of workers exposed to trinitrotoluene (TNT) ANALYSES OF TNT, 4-ADNT, AND 2-ADNT 2,4,6-TNT, 4-ADNT, and 2-ADNT were analysed in the same urine samples using the method described by Allmog et al 8 A portion of the urine samples were concentrated on XAD-2 resin in the same way as in the mutagenicity assay. One millilitre of concentrate was adjusted to pH 2 with diluted HCL.…”

Section: Methods and Exclusions Bacterial Mutagenicity Assay Of Urinementioning

confidence: 99%

Mutagenic activity and metabolites in the urine of workers exposed to trinitrotoluene (TNT).

Ahlborg

Einistö²,

Sorsa³

1988

Occupational and Environmental Medicine

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Urine samples taken after work and after a free weekend from 50 workers employed in various activities in a chemical plant manufacturing explosives were analysed. On the basis of hygienic surveys, the subjects were divided into three categories ofexposure to trinitrotoluene (TNT). The urine analyses consisted of gas chromatographic identification of TNT and its two metabolites, 4-ADNT and 2-ADNT, and a determination of the mutagenic activity. Two frame shift detector strains of Salmonella typhimurium were used, TA 98 and TA 98 NR, the latter being deficient in endogenous nitroreductase activity. On the basis of previous results on TNT mutagenicity, no exogeneous metabolic system was used to test the urine concentrates. Both tester strains showed that the mean urinary mutagenic activity was higher in the after work samples than in post weekend samples from the same subjects, showing that bacterial nitroreductase activity was not significantly responsible for the mutagenicity, although the response was higher with strain TA 98 than with TA 98 NR. The interindividual variation in urine mutagenicity was high, however, and the difference between the two sampling times was statistically significant (p < 005) only for the high exposed group (workers in trotyl foundry and sieve house). Correlation between urinary mutagenicity and concentration of TNT in urine was poor; correlation was significant only with the urinary concentration of 4-ADNT. The correlation between urinary TNT and both metabolites was good (p < 0O001). These results suggest that analysis of 4-ADNT in urine would be a sufficient biological measure for controlling exposure to TNT.

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Section: Methods and Exclusions Bacterial Mutagenicity Assay Of Urinementioning

confidence: 99%

Mutagenic activity and metabolites in the urine of workers exposed to trinitrotoluene (TNT).

Ahlborg

Einistö²,

Sorsa³

1988

Occupational and Environmental Medicine

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“…[20]. Zusammenfassend ist festzustellen, daß 2,6-Dinitro-4-aminotoluol (2,6-DN-4-AT) der Hauptmetabolit ist (Arbeiter: 0,96-2,09 mg 2,6-DN-4-AT/24 Stunden [23], Arbeiter: 10 -4""""" �10 -3 g 2,6-DN-4-AT/1 Harn [24]; in beiden Untersuchungen keine Angaben zur Expositionshöhe). Da auch andere Dinitroaminotoluole und 2,6-Dinitro-4-hydroxylaminotoluol ausgeschieden werden, sind in erster Linie Reduktionsprozesse zu vermuten [4, 17, 20, 22, 25,-30].…”

Section: Angaben Zur Pharmakokinetikunclassified

2,4,6‐Trinitrotoluol (und Isomeren in technischen Gemischen) [MAK Value Documentation in German language, 1988]

2012

The MAK‐Collection for Occupational Health and Safety

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Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 14. Lieferung, Ausgabe 1988 Der Artikel enthält folgende Kapitel: Charakterisierung der einzelnen Trinitrotoluole und des technischen Gemisches Allgemeiner Wirkungscharakter Angaben zur Pharmakokinetik Erfahrungen beim Menschen Akute Toxizität Hauttoxizität Subchronische und chronische Toxizität Blut‐ und Leberschädigung nach t‐TNT‐Exposition Augenschädigung nach t‐TNT‐Exposition ‐ „TNT‐Star“ Tierexperimentelle Befunde Akute und subakute Toxizität Hauttoxizität Subchronische und chronische Toxizität Untersuchungen zur gentoxischen Wirkung Untersuchungen zur kanzerogenen Wirkung Zur Frage eines MAK‐Wertes

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“…There are tests to determine if you have been exposed to 2,4,6-trinitrotoluene. These tests measure 2,4,6-trinitrotoluene or its breakdown products in blood and urine and have been used to test exposed workers [5]. Detection of the breakdown products in urine is a clear indication that person has been exposed.…”

Section: Introductionmentioning

confidence: 99%

“…The complex and expensive equipment needed to perform these tests is generally available only at specialized laboratories [6]. Another simpler, but less specific, test of 2,4,6-trinitrotoluene exposure is a change in the color of urine to amber or deep red [5]. This change results from the presence of breakdown products and may indicate that person has been exposed to 2,4,6trinitrotoluene.…”

Section: Introductionmentioning

confidence: 99%

Interaction of TNT with Dopamine - A DFT Treatment

Türker

2019

EJCS

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2,4,6-trinitrotoluene, known as TNT is a widely used high explosive material. Its disastrous effects to health are reported but how it executes those effects biochemically has been scarcely studied. On the other hand, dopamine is an important endogenous catecholamine. The present study deals with interaction of TNT and dopamine within the restrictions of density functional theory at the level of B3LYP/6-31+G(d). Some structural, quantum chemical and spectral properties are obtained for TNT + dopamine composite. They may form two electronically stable π-π complex, TNT acting as π-acceptor and dopamine is the donor. Thermo chemical calculations indicate that formation of the complexes are unfavorable at the standard conditions but the endo-form of the complex is less unfavorable compared to the exo-form.

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Determination of T.N.T. Metabolites in Urine

Cited by 12 publications

References 3 publications

Mutagenic activity and metabolites in the urine of workers exposed to trinitrotoluene (TNT).

Mutagenic activity and metabolites in the urine of workers exposed to trinitrotoluene (TNT).

2,4,6‐Trinitrotoluol (und Isomeren in technischen Gemischen) [MAK Value Documentation in German language, 1988]

Interaction of TNT with Dopamine - A DFT Treatment

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