Determination of Terminal Sequences in Viral and Ribosomal Ribonucleic Acids

Lee, JC; Pt, Gilham

doi:10.1021/ja01095a051

Cited by 14 publications

(4 citation statements)

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“…The results of others (Lane, 1962; Lane and Allen, 1961; Lee and Gilham, 1965;Singh and Lane, 1964), who found all four nucleosides in alkaline hydrolysates of wheat germ rRNA, appeared to be different from our findings of only adenosine and uridine in hydrolysates of plant rRNA. In an attempt to explain this difference, wheat germ rRNA was isolated by direct phenol extraction and hydrolyzed both by the methods employed in our laboratory and those described by Singh and Lane (1964).…”

Section: Resultscontrasting

confidence: 99%

“…Only adenosine and uridine were detected in the hydrolysate from preparation I, whereas 5% of the total nucleoside in preparation II was cytidine, although adenosine and uridine again predominated. The hydrolysate of wheat germ rRNA, prepared and hydrolyzed by the methods of Singh and Lane (1964) (preparations III and IV), were found to contain all four nucleosides, as had been previously reported (Lane, 1962; Lane and Allen, 1961; Lee and Gilham, 1965;Singh and Lane, 1964). These results are presented in Table II.…”

Section: Resultssupporting

confidence: 72%

“…Wheat germ rRNA, the only plant rRNA studied prior to our investigation, also provides the only example to date of an RNA on which no preferential termination at either terminus has been observed, as all four nucleoside diphosphates were derived from the 3'linked terminus in alkaline hydrolysates (Singh and Lane, 1964;Lane, 1965) and all four nucleosides were similarly derived from the 5'-linked terminus (Lane and Allen, 1961;Singh and Lane, 1964;Lane, 1965; Lee and Gilham, 1965). Information concerning the termini of rRNA has been reviewed by Madison (1968).…”

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The 5'-linked termini of plant ribosomal ribonucleic acids

Halloin¹,

Pollard²,

Becker³

et al. 1969

Biochemistry

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Section: Resultscontrasting

confidence: 99%

Section: Resultssupporting

confidence: 72%

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The 5'-linked termini of plant ribosomal ribonucleic acids

Halloin¹,

Pollard²,

Becker³

et al. 1969

Biochemistry

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“…The various aspects of determining the nucleic acid sequences and termini also have been recently reviewed by RajBhandary and Stuart (17). Several papers have been published dealing with the identification of the 5'-linked termini of ribosomal RNA (7,9,10,11,13,15). From the studies on these termini, the results most consistently obtained are that there is a preferential termination of ribosomal RNA by adenosine and uridine.…”

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confidence: 99%

The Use of Nucleoside Phosphotransferase and (³²P) p-Nitrophenyl Phosphate in the Determination of the 5′-Linked Termini of Ribosomal RNA

Becker

Pollard

1969

Plant Physiol.

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(2,3,16). Since the artificial substrate p-nitrophenyl phosphate could serve as a donor, and it appeared that radioactive p-nitrophenyl phosphate could be synthesized, it was felt that the enzymatic synthesis of radioactive nucleotides utilizing (32P) p-nitrophenyl phosphate and the enzyme could prove to be tuseful in the identification of nucleosides.In studies on the terminal groups of ribosomal RNA it is either necessarv to have large quantities of RNA available or to utilize techniques capable of detecting small amounts of the terminal groups. Often it is not feasible to obtain large quantities of material and con,sequently the latter method must be pursued. This paper then reports the isolation and partial purification of nucleoside phosphotransferase from carrot leaves, the synthesis of (32p) For a discussion concerning the formation, structure, and function of ribosomal RNA the reader is referred to the reviews of Madison (12) and Osawa (16). The various aspects of determining the nucleic acid sequences and termini also have been recently reviewed by RajBhandary and Stuart (17). Several papers have been published dealing with the identification of the 5'-linked termini of ribosomal RNA (7,9,10,11,13,15). From the studies on these termini, the results most consistently obtained are that there is a preferential termination of ribosomal RNA by adenosine and uridine. Exceptions to the rule are the investigations on wheat germ ribosomal RNA (9, 11) from whence equal amounts of each of the 4 nucleosides were isolated. Recently, evidence was presented from this laboratory which indicated that adenosine and uridine are the predominant 5'-linked termini of wheat germ ribosomal RNA as well as ribosomal RNA from 4 other higher plants and a fungus (6). Materials and Methods

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