Determination of Testosterone in Human Peripheral and Adrenal Venous Plasma

Burger, Henry; Kent, J. R.; Kellie, A. E.

doi:10.1210/jcem-24-5-432

Cited by 94 publications

(22 citation statements)

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“…The plasma testosterone levels of the adult male are quite comparable to those reported by others using either double isotope derivative techniques (6)(7)(8) or the fluorescence of the estrogen formed from testosterone (9). The reliability of the present method for studies of Leydig cell physiology is adequate.…”

Section: Discussionsupporting

confidence: 82%

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Plasma Ketosteroids and Testosterone in Man: A Study of the Pituitary-testicular Axis*

Kirschner¹,

Lipsett²,

Collins³

1965

J. Clin. Invest.

122

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The relationship between the Leydig cell of the testis and the tropic hormones of the anterior pituitary gland has been inadequately explored in man because of methodological difficulties. The development of methods for measuring plasma testosterone 1 levels in this and other laboratories has made such studies possible. We have examined the ability of the Leydig cell to respond to stimulation by gonadotropin, its inhibition by exogenous androgen, diurnal variation of plasma testosterone, and the role of the adrenal cortex in the maintenance of plasma testosterone levels in man.To measure plasma testosterone, we have used a double isotope derivative method and employed gas-liquid chromatography as the final chromatographic step. This method has also been applied to the measurement of the plasma levels of dehydroepiandrosterone, androsterone, and etiocholanolone. The methods will be presented in detail, and we shall show that the relationship between the testicular Leydig cell and the anterior pituitary gland is similar to the relationship between the adrenal cortex and the anterior pituitary gland. MethodsThe plasma steroids were measured by a double isotope derivative method using C14-labeled steroids to monitor recovery and H'-acetic anhydride to measure mass.

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Section: Discussionsupporting

confidence: 82%

“…After The eluates were dried in 15-ml glass-stoppered centrifuge tubes. Acetylation was performed with 10 ,ul of pyridine and 10 Al of 20% acetic anhydride-l-H' in benzene and overnight incubation at 370 C. 5 Packard Instruments Co., La Grange, Ill. 6 Applied Science Laboratories, State College, Pa. 7 Pilot Chemicals, Watertown, Mass.…”

Section: Methodsmentioning

confidence: 99%

Plasma Ketosteroids and Testosterone in Man: A Study of the Pituitary-testicular Axis*

Kirschner¹,

Lipsett²,

Collins³

1965

J. Clin. Invest.

122

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“…2 Early serum testosterone assays used double-isotope-derivative dilution with a thin-layer chromatography modification and were limited in their accuracy and sensitivity. [3][4][5] The limits of measurement imposed by these assays led to the target testosterone suppression definition of less than 1.7 nmol/L; 4,6 however, more recent studies measuring serum testosterone levels after surgical castration with the use of more modern techniques, using improved radioimmunoassay (RIA) and chemiluminescent immunoassay (CLIA) and mass spectrometry (MS) methods, have reported mean testosterone levels as low as 0.003 nmol/L. 4,[7][8][9][10][11] Several studies since the early 1990s have therefore challenged the outdated benchmark of 1.7 nmol/L and recommended revisiting the definition, with many suggesting a new benchmark of 0.7 nmol/L.…”

Section: Introductionmentioning

confidence: 99%

Testosterone monitoring for men with advanced prostate cancer: Review of current practices and a survey of Canadian physicians

Shayegan

Pouliot

et al. 2017

CUAJ

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Androgen-deprivation therapy (ADT) is a standard of care in the treatment of advanced prostate cancer; however, testosterone monitoring practices for men undergoing ADT vary across Canada. Although a testosterone level of 1.7 nmol/L or lower has historically been defined as the accepted castrate level, newer assays with improved sensitivity have shown that both medical and surgical castration can suppress testosterone levels to below 0.7 nmol/L. This review explores the evidence supporting a redefinition of the castrate testosterone level as 0.7 nmol/L or lower, and presents results of a survey of testosterone monitoring practices among 153 Canadian urologists, uro-oncologists, and radiation oncologists who manage the treatment of men with hormone-sensitive prostate cancer.

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“…In several series of hirsute women (1)(2)(3)(4)(5)(6), the average plasma testosterone concentration was high, but in each series some of the subjects had normal levels. The role of plasma androstenedione has not been evaluated in hirsute women, and there is no comprehensive study of androgen production rates in such patients.…”

Section: Introductionmentioning

confidence: 99%

Testosterone and Androstenedione Blood Production Rates in Normal Women and Women with Idiopathic Hirsutism or Polycystic Ovaries*

Bardin¹,

Lipsett²

1967

J. Clin. Invest.

323

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Summary. The average plasma testosterone concentration of women with either hirsutism or polycystic ovaries and hirsutism was higher (p < 0.01) than that of normal women although the ranges overlapped. Testosterone blood production rates averaged 830 ± 120 SE and 1,180 ± 310 SE ,ug per day in the two groups of hirsute women and 230 ± 33 SE ,ug per day in normal women. The ranges did not overlap.The testosterone metabolic clearance rates of hirsute women (1,090 ± 140 SE L per day) and of men (1,240 + 136 SE L per day) were significantly higher than those of normal women (590 + 44 SE L per day). These differences persisted when the metabolic clearance rates were corrected for surface area. We suggest that testosterone metabolic clearance rates vary directly with some function of testosterone production.The mean plasma androstenedione levels (2.8 ± 0.35 SE and 2.8 + 0.30 SE ,ug per L) and production rates (6,060 ± 450 SE and 7,360 + 345 SE ,ug per day) of the women with hirsutism or polycystic ovaries, respectively, were significantly higher than those of normal women (1.5 + 0.22 SE pg per L; 3,300 + 830 SE pg per day). The androstenedione metabolic clearance rates were the same in each group. Plasma androstenedione was the precursor of 49% of plasma testosterone in normal women and of 26% of plasma testosterone in hirsute women. Thus, 74% of the plasma testosterone in these subjects must have been either secreted or derived from a precursor that did not enter the plasma androstenedione pool.

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Determination of Testosterone in Human Peripheral and Adrenal Venous Plasma

Cited by 94 publications

References 1 publication

Plasma Ketosteroids and Testosterone in Man: A Study of the Pituitary-testicular Axis*

Plasma Ketosteroids and Testosterone in Man: A Study of the Pituitary-testicular Axis*

Testosterone monitoring for men with advanced prostate cancer: Review of current practices and a survey of Canadian physicians

Testosterone and Androstenedione Blood Production Rates in Normal Women and Women with Idiopathic Hirsutism or Polycystic Ovaries*

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