Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection

Pena, Angelina; López-Carmona, Alicia; Barbosa, Antonio Marcos Jacques; Lino, Celeste M.; Silveira, I.; Castillo, B. del

doi:10.1016/s0731-7085(98)00268-4

Cited by 55 publications

(30 citation statements)

References 17 publications

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“…The results obtained 16 ANALYTICAL SCIENCES JANUARY 2006, VOL. 22 by the present experiments were in good agreement with those reported by Pena et al 40 The stability of tetracycline was poor under strong acidic and alkaline conditions along with a reversible formation of epimers at position C4 to 4-epitetracycline in weak acid and anhydrotetracycline under strong acidic conditions. 40,41 The effect of the pH of the buffer stream on various concentrations of tetracycline (2.0 -20.0 µg ml -1 ) was also investigated.…”

Section: Effect Of the Phsupporting

confidence: 93%

Flow Injection Spectrophotometric Determination of Tetracycline in a Pharmaceutical Preparation by Complexation with Aluminium(III)

Liawruangrath

Watanesk

et al. 2006

ANAL. SCI.

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Tetracycline is one of the broad-spectrum antibiotics 1,2 having a fused, partially aromatic, 4-ring structure with a wide variety of functional groups that form complexes with various metal ions in aqueous solution. This ability is responsible for its antibacteria action. 3,4 Numerous methods have been reported for determining tetracycline in many matrices, for example: microbiological, 5,6 electrochemical, [7][8][9][10][11] chromatographic, 12-17 capillary electrophoresis, 18,19 spectrophotometric, 20-23 fluorometric, 24,25 and chemiluminescence [26][27][28][29] procedures. The microbiological assay is sensitive, but requires a long period of incubation and lacks precision and specificity. Some spectrophotometric methods are rather insensitive and subject to interference from other materials. The fluorometric method is less sensitive than the microbiological method, but is specific for tetracycline. Other methods require elaborate instrumentation or have a low sampling frequency.Most spectrophotometric and fluorometric methods, 30-34 including chromatographic methods with spectrophotometric and fluorometric detections 35,36 for the determination of tetracyclines, are based on their abilities to bind with metals, such as iron(III), 30,31 copper(II), 32 magnesium(II) 33,34 and some lanthanide ions, especially those of europium, samarium and terbium. 34 Since the conventional spectrophotometric methods for tetracycline determinations are tedious and time consuming, flow injection analysis (FIA) methods with chemiluminescence [26][27][28][29] and spectrophotometric 32,37 detection have been employed instead. The FI method is suitable for rapid quality control in drug industries where these drugs are manufactured because of its high sample throughput, which enables a large number of samples to be determined with cost effectiveness. This paper describes a rapid, sensitive, and reproducible flow injection (FI) method for the quantitation of tetracycline based on the spectrophotometric detection of the colored product formed by the reaction between this drug and aluminum(III) in a Tris-buffer pH 7 medium. The resulting Al(III)-tetracycline complex is measured at 376 nm. Experimental Reagents and solutionAll chemicals were of analytical reagent grade and were used without further purification. Deionized distilled water was used throughout the whole experiment. A standard stock solution of tetracycline (100 mg l -1 ) was prepared by dissolving tetracycline (0.10 g obtained from Fluka Switzerland) in 1 L water. Futher dilutions with water were made for appropriate concentrations. A 0.0100 mol l -1 aluminum(III) chloride hexahydrate (BDH Chemical Poole, England) was prepared in 1 L of water. A rapid flow injection (FI) spectrophotometric procedure for tetracycline determination is described. It is based on the injection of a 100 µl sample solution containing tetracycline into merged streams of aluminium(III) chloride (0.01 mol l -1 ) and Tris-buffer in the presence of KCl (0.06 mol l -1 ), pH 7.0, with the same optimum f...

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Section: Effect Of the Phsupporting

confidence: 93%

Flow Injection Spectrophotometric Determination of Tetracycline in a Pharmaceutical Preparation by Complexation with Aluminium(III)

Liawruangrath

Watanesk

et al. 2006

ANAL. SCI.

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“…Tetracycline antibiotics are commonly used all over the world with broad antibacterial spectrum and bacteriostatic activity, as medicines for humans and animals, and as feed additives since they are well absorbed, exhibit low toxicity and are relatively inexpensive. [1][2][3][4][5][6][7][8] The most common tetracyclines (TC) applied to food-producing animals are tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC) and doxycycline (DC) (Figure 1). [9][10][11][12][13] The high and in some cases improper use of TC antibiotics, may result in the presence of residues in edible animal tissues, which can be toxic and dangerous for human health and potencially cause allergic reactions in some hypersensitive individuals.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a method for the analysis of tetracyclines in chicken-muscle by liquid chromatography-electrospray-mass spectrometry in tandem (LC-ESI-MS/MS)

Uekane¹,

Neto²,

Gomes³

2011

Quím. Nova

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Recebido em 20/12/09; aceito em 8/7/10; publicado na web em 9/11/10 A LC-ESI-MS/MS method was developed and validated according to the European Union decision 2002/657/EC, for the determination of tetracyclines (TCs) in chicken-muscle since Europe is one of the main markets for Brazilian products. Linearity of r > 0.9979, limits of quantification in the range of 7.0-35.0 ng/g, average recoveries of 89.38 -106.27%, within-day and betweenday precision were adequate for all TCs. The decision limit and the detection capability were 93.00-106.46 ng/g and 95.84-114.38 ng/g, respectively. This method is suitable for application in surveillance programmes of residues of TCs in chicken-muscle samples.

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“…Owing to such a broad spectrum of action, they are widely used to combat infections and human disease and also for veterinary therapy [4][5][6]. Several methods have been reported for their determinations, both in pharmaceutical formulations and biological samples (fluids and tissues) including methods such as spectrophotometric [7][8][9][10], amperometric [11,12], chemiluminescence [13,14], spectrofluorimetric [15,16] and liquid chromatographic [17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T

et al. 2010

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A simple, rapid, accurate and inexpensive spectrophotometric method for the determination of tetracycline and doxycycline has been developed. The method is based on the reaction between these drugs and chloramine-T in alkaline medium producing red color products with absorbance maximum at the ë = 535 and 525 nm for the tetracycline and doxycycline, respectively. The best conditions for the reactions have been found using mol L and 7.12 x 10 mol L for the tetracycline and doxycycline, respectively. The proposed method was successfully applied to the determination of these drugs in pharmaceutical formulations and the results obtained were in good agreement with those obtained by the comparative method at the 95% confidence level.

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Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection

Cited by 55 publications

References 17 publications

Flow Injection Spectrophotometric Determination of Tetracycline in a Pharmaceutical Preparation by Complexation with Aluminium(III)

Flow Injection Spectrophotometric Determination of Tetracycline in a Pharmaceutical Preparation by Complexation with Aluminium(III)

Development and validation of a method for the analysis of tetracyclines in chicken-muscle by liquid chromatography-electrospray-mass spectrometry in tandem (LC-ESI-MS/MS)

A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T

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