Determination of tetracycline, oxytetracycline and chlortetracycline residues in seafood products of Saudi Arabia using high performance liquid chromatography–Photo diode array detection

Alanazi, Fatimah; Almugbel, Rawan; Maher, Hadir M.; Alodaib, Faten M.; Alzoman, Nourah Z.

doi:10.1016/j.jsps.2021.04.017

Cited by 34 publications

(23 citation statements)

References 27 publications

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“…Two grams of each sample was weighed into a polypropylene tube after it was cut into small pieces and homogenized, and then 0.8 mL of 20% trifluoroacetic acid and 0.4 mL of 0.01 M EDTA were added. 7 , 25 Then, the extracts were heated in a water bath at 54°C to inactivate the complement system and other natural antimicrobial systems. The mixture was vortexed for 2 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…The supernatant was filtered through a 0.22 µm nylon filter and injected into the HPLC for analysis. 7 …”

Section: Methodsmentioning

confidence: 99%

“… 1 , 6 Tetracyclines are antibiotics that include tetracycline, oxytetracycline, chlortetracycline, doxycycline, and minocycline. 7 - 9 …”

Section: Introductionmentioning

confidence: 99%

“… 11 , 13 , 14 It has many consequences, such as increasing morbidity (pose toxic and dangerous effects on the consumer’s health; allergic reactions, poor fetal development, and gastrointestinal effects) and mortality rates, as well as increasing hospital stays, and leading to an increase in medical costs. 7 , 15 - 17 …”

Section: Introductionmentioning

confidence: 99%

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Determination of Tetracycline Residues in Kidney and Muscle of Beef Cattle Slaughtered in Dire Dawa and Harar Municipal Abattoirs, Eastern Ethiopia

Mohammed¹,

Mengistu

Abdurehman

et al. 2022

Environ�Health�Insights

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Background: Antibiotics are among the most widely used drugs in farm animals for therapeutic and prophylactic purposes. However, the antibiotic residues in meat are a serious public health concern due to their harmful effects on consumer health. Besides this problem, there is limited information on the level of the antibiotic residues, including tetracycline residues. Therefore, this study was aimed to determine the tetracycline residues in the kidney and muscle samples of beef cattle in Harar town and Dire Dawa city. Methods: A study was conducted on 500 randomly selected carcass (250 kidney and 250 muscle samples) slaughtered at Dire Dawa and Harar municipal slaughterhouses between December 2018 and December 2019. The samples were collected aseptically and screened for tetracycline residues by thin layer chromatography. Then, presumptive positive samples were analyzed using high-performance liquid chromatography to get a quantitative outcome. Descriptive statistics were used to determine the frequency, mean, or standard deviation to determine the summary values and distribution of the outcomes. Finally, the data was analyzed using SPSS version 21 software. Results: Out of 500 samples, oxytetracycline residues were detected in 84% of the samples. However, tetracycline and doxycycline were not detected in all samples. Among the kidney and muscle samples collected from Dire Dawa and Harar abattoirs, 109 (87.2%) and 101 (80.8%) were positive for oxytetracycline, respectively. Oxytetracycline residue levels in Dire Dawa ranged from 57 to 607 µg/kg for the kidney and 10.14 to 435 µg/kg for muscle samples. Among the samples collected from Harar, the concentration of oxytetracycline residues ranged from 16 to 433 µg/kg and 6 to 435 µg/kg for kidney and muscle samples, respectively, at Harar slaughterhouses. About 22.0% of muscle samples collected from Dire Dawa and 17.8% from Harar town had oxytetracycline residues above maximum residue limits. Conclusions: In general, the study revealed that oxytetracycline residues were prevalent among tetracycline residues analyzed from kidney and muscle samples in the study areas. Thus, there is a risk of consumer exposure to these antibiotic residues that may have human health effects. Therefore, awareness creation and strict regulation is needed by the regulatory authorities for the use of antimicrobial drugs in the livestock industry.

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Section: Methodsmentioning

confidence: 99%

“…The supernatant was filtered through a 0.22 µm nylon filter and injected into the HPLC for analysis. 7 …”

Section: Methodsmentioning

confidence: 99%

“… 1 , 6 Tetracyclines are antibiotics that include tetracycline, oxytetracycline, chlortetracycline, doxycycline, and minocycline. 7 - 9 …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determination of Tetracycline Residues in Kidney and Muscle of Beef Cattle Slaughtered in Dire Dawa and Harar Municipal Abattoirs, Eastern Ethiopia

Mohammed¹,

Mengistu

Abdurehman

et al. 2022

Environ�Health�Insights

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“…TC, oxytetracycline (OTC), and chlortetracycline (CTC) are commonly used in medicine [24]. They exhibit various advantages including a high oral bioavailability, low price, and accessibility [13,[25][26][27][28]. TCs have a high affinity to HAp.…”

Section: Introductionmentioning

confidence: 99%

Sorption Studies of Tetracycline Antibiotics on Hydroxyapatite (001) Surface—A First-Principles Insight

et al. 2022

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Owing to the limitations of traditional systemic drug delivery in the treatment of bone diseases with side effects on normal cells, the selection of materials with high affinities for bones, as targeting ligands to modify drug carriers, has become an important research topic. Tetracyclines (TCs) have an adsorption effect on hydroxyapatite (HAp). Thus, they can be used as bone-targeting ligands and combined with drug carriers. In this study, density functional theory is used to analyze the interaction mechanism of TC, oxytetracycline (OTC), chlortetracycline, and HAp. We calculate the electrostatic potential (ESP) and molecular orbitals to predict the possible binding sites of TCs on the HAp surface. The adsorption energy is used to compare the affinities of the three TCs to HAp. An independent gradient model analysis is performed to study the weak interaction between TCs and HAp. The coordination bond between TCs and the HAp surface is evaluated by conducting a charge density difference analysis. The results show that OTC has the highest affinity to HAp because the introduction of hydroxyl groups change the adsorption configuration of OTC. Thus, OTC adsorbed on HAp in a broken-line shape exposes more binding sites. This study provides a theoretical basis for TCs as bone-targeting ligands in treating bone diseases and in improving the safety of treatment by selecting different bone-targeting ligands.

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Recent review on porous adsorbents for water decontamination: strategies for enhanced removal of tetracycline

Zheng,

Teo,

Seenivasagam

et al. 2024

J Porous Mater

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Determination of tetracycline, oxytetracycline and chlortetracycline residues in seafood products of Saudi Arabia using high performance liquid chromatography–Photo diode array detection

Cited by 34 publications

References 27 publications

Determination of Tetracycline Residues in Kidney and Muscle of Beef Cattle Slaughtered in Dire Dawa and Harar Municipal Abattoirs, Eastern Ethiopia

Determination of Tetracycline Residues in Kidney and Muscle of Beef Cattle Slaughtered in Dire Dawa and Harar Municipal Abattoirs, Eastern Ethiopia

Sorption Studies of Tetracycline Antibiotics on Hydroxyapatite (001) Surface—A First-Principles Insight

Recent review on porous adsorbents for water decontamination: strategies for enhanced removal of tetracycline

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