Determination of tetracyclines in pig and other meat samples using liquid chromatography coupled with diode array and tandem mass spectrometric detectors

Tölgyesi, Ádám; Tölgyesi, László; Békési, Katalin; Sharma, Virender K.; Fekete, Jenő

doi:10.1016/j.meatsci.2013.11.011

Cited by 35 publications

(25 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many articles report on various challenges encountered when developing a method for tetracyclines antibiotics including tigecycline determination [4]. In a simple biological sample treatment method and the analysis of relatively short time, a method for simultaneously quantifying tigecycline and its epimer becomes more difficult to achieve good separation.…”

Section: Fig 1 Chemical Structure Of Tigecycline (A) Its Epimer (Bmentioning

confidence: 99%

Development and Validation of an LC–MS/MS Method for Determination of Tigecycline and Its Epimer in Human Plasma and Its Application in a Pharmacokinetic Study

Wang¹,

Hu²,

Zhu³

et al. 2016

Acta Chromatographica

View full text Add to dashboard Cite

Summary.A rapid, selective, sensitive, and simple method for simultaneous determination of tigecycline and its epimer in human plasma samples was developed and validated by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Sample preparation involved one-step protein precipitation by adding 0.1% formic acid-methanol and phosphate buffer (PB) solution to the plasma. Chromatographic separation was obtained with XBridge BEH C18 column (3.5 μm, 50 × 4.6 mm) through a 9.5-min gradient mobile phase at the flow rate of 0.6 mL min −1 at 4 °C. The calibration curves were linear over concentration 5.00-2000 ng mL −1 with correlation coefficient greater than 0.998. Intra-batch and inter-batch accuracy of the assay were in the ranges of −2.90% to 3.00%, and the corresponding precision was less than 6.97%. The extraction recovery of tigecycline and its epimer with the current method were 87.2% and 76.9%, respectively. The applied LC-MS/MS method was shown to be sufficiently sensitive and will be suitable for pharmacokinetic studies.

show abstract

Section: Fig 1 Chemical Structure Of Tigecycline (A) Its Epimer (Bmentioning

confidence: 99%

Development and Validation of an LC–MS/MS Method for Determination of Tigecycline and Its Epimer in Human Plasma and Its Application in a Pharmacokinetic Study

Wang¹,

Hu²,

Zhu³

et al. 2016

Acta Chromatographica

View full text Add to dashboard Cite

show abstract

“…Liquid extraction from solid matrices seems the most common method for the separation of tetracy clines from the solid samples of food products. This method is used for the extraction of tetracycline anti biotics from meat [15][16][17][18][19][20][21][22][23][24][25][26][27], fish [27][28][29][30][31], eggs [18,27,32], and honey [33][34][35][36][37][38][39], and it is performed as follows: A weighed portion of a thoroughly ground solid sam ple is placed in a vessel for agitation, and a selected sol vent is added; the contents are stirred for a specified time (from several minutes to several hours). The phases are separated by filtration.…”

Section: Sample Preparationmentioning

confidence: 99%

“…The phases are separated by filtration. Phosphate [16-18, 33, 38], citrate [19,29,33], hexamethylene tetramine [39], oxalate [20], and succinate [21,32] buffer solu tions and also the McIlvaine buffer solution [15,[34][35][36][37] are most frequently used as solvents. Furthermore, 5% trichloroacetic acid [23], a mixture of acetonitrile with a citrate buffer [28] and the McIlvaine buffer solution (60 : 40) [24,31], and the mixtures of metha nol with water (70 : 30) [30] and with 0.1 M succinic acid (50 : 50) [22,27] or acetonitrile are used [25,26].…”

Section: Sample Preparationmentioning

confidence: 99%

Methods for the separation, preconcentration, and determination of tetracycline antibiotics

2015

View full text Add to dashboard Cite

The data published in the last five years concerning the determination of tetracycline antibiotics in different samples are generalized. Sample preparation procedures for real samples, including liquid extrac tion from solid matrices, pressurized liquid extraction, matrix solid phase dispersion, QuEChERS method, and solid phase extraction, are compared. The following important determination techniques are discussed: chromatographic, electrophoretic, spectroscopic, and electrochemical. The determination of the above anti biotics in food products, feed, environmental samples, pharmaceuticals, and biological fluids is exemplified.

show abstract

“…The usual methods that were used for determination of TCs are the chromatographic techniques, specially the liquid chromatography methods (Zheng et al 2012;Tölgyesi et al 2014;Xu et al 2008). Regarding the high development in stationary phases and operation of detectors in liquid chromatography, the analysis of low concentrations of TCs in food samples is still a difficult subject.…”

Section: Introductionmentioning

confidence: 99%

Determination of tetracyclines in meat using two phases freezing extraction method and HPLC-DAD

2014

View full text Add to dashboard Cite

The protein binding of tetracyclines may cause the common extraction methods are not able to completely remove them from the proteins. To overcome this problem, we introduced a simple, reliable, sensitive, and reproducible method for simultaneous clean-up and preconcentration of 4 tetracyclines in chicken meats based on two phase freezing method.The tetracyclines were extracted by solid liquid extraction using Britton-Robinson buffer (0.008 mol L −1 , pH=10) from chicken breast, thigh and liver samples. Then simultaneous separation, clean up, protein-drug bonds breaking and preconcentration of tetracyclines from extract solution was performed by two phase freezing method. In this method the acetonitrile was mixed with aqueous extractant solution, the mixture solution was frozen and the organic phase was removed by centrifuge at −20°C. For separation of tetracyclines a reversed-phase high-performance liquid chromatography was used. In two phase freezing method several parameters such as pH of extract solution, the type of miscible organic solvent, salting out, the ratio of organic/aqueous phases, and extraction cycles were investigated. The recoveries of tetracyclines spiked at three levels of concentration (50, 100, and 150 μg Kg −1 ) were changed from 76.5 % to 95.5 % with the relative standard deviation values less than 11 %. This method is a reliable tool and could be applied to analyze tetracyclines residues in chicken meats.

show abstract

Determination of tetracyclines in pig and other meat samples using liquid chromatography coupled with diode array and tandem mass spectrometric detectors

Cited by 35 publications

References 32 publications

Development and Validation of an LC–MS/MS Method for Determination of Tigecycline and Its Epimer in Human Plasma and Its Application in a Pharmacokinetic Study

Development and Validation of an LC–MS/MS Method for Determination of Tigecycline and Its Epimer in Human Plasma and Its Application in a Pharmacokinetic Study

Methods for the separation, preconcentration, and determination of tetracycline antibiotics

Determination of tetracyclines in meat using two phases freezing extraction method and HPLC-DAD

Contact Info

Product

Resources

About