Determination of the 5-fluorouracil and N1(2′-furanidyl)uracil in the presence of tegafur by zero-crossing first derivative spectrometry

Badea, Irinel Adriana; Moja, Dana; Tudose, Aurel; Stoicescu, Dan

doi:10.1016/s0731-7085(02)00436-3

Cited by 9 publications

(4 citation statements)

References 13 publications

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“…Several methods are described in the literature for the determination of 5-FU in samples of biological matrices using gas chromatography/mass spectrometry (GC/MS) (Anderson et al, 1997), spectrometry (Badea et al, 2002), high performance liquid chromatography (HPLC) (Escoriaza et al, 1999), hydrophilic interaction liquid chromatography-APCI-mass spectrometry (Pisano et al, 2005) or liquid chromatography tandem mass spectrometry (LC-MS/MS) (Licea-Perez, Wang, Bowen, 2009;Liu et al, 2010). The analytical determination of 5-FU in pharmaceutical dosage forms such as nanoparticles has been performed by several authors using spectrophotometry (Bozkir, Saka, 2005;Liu et al, 2006;Zhu et al, 2009;Lai, Guo, 2011;Li et al, 2011;Rejinold et al, 2011a;Rejinold et al, 2011b;Zhang et al, 2011), but few studies report the use of HPLC methods for this determination.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles

Mattos

Khalil

Mainardes

2013

Braz. J. Pharm. Sci.

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The objective of this work was to develop and validate a rapid high performance liquid chromatography (HPLC) method for the quantitative analysis of fluorouracil (5-FU) in polymeric nanoparticles. Chromatographic analyses were performed on an RP C18 column with a mobile phase consisting of acetonitrile and water (10:90, v/v) at a flow rate of 1 mL/min. The 5-FU was detected and quantitated using a photodiode array detector at a wavelength of 265 nm. The method was shown to be specific and linear in the range of 0.1-10 μg/mL (r = 0.9997). The precision (intra-and inter-day) was demonstrated because the maximum relative standard deviation was 3.51%. The method is robust relative to changes in flow rate, column and temperature. The limits of detection and quantitation were 10.86 and 32.78 ng/mL, respectively. The method fulfilled the requirements for reliability and feasibility for application to the quantitative analysis of 5-FU in polymeric nanoparticles.Uniterms: Fluorouracil/determination. Nanoparticles. High performance liquid chromatography/ quantitative analysis/method validation.O objetivo deste trabalho foi desenvolver e validar um método rápido de cromatografia líquida de alta eficiência (CLAE) para análise quantitativa de fluorouracila (5-FU) em nanopartículas poliméricas. Corridas cromatográficas foram realizadas sob uma coluna RP C18 com uma fase móvel consistindo de acetonitrila e água (10:90, v/v) a um fluxo de 1 mL/min. O 5-FU foi detectado e quantificado através de um detector de fotodiodos em um comprimento de onda de 265 nm. O método demonstrou ser específico e linear na faixa de 0,1-10 μg/mL (r =0.9997). As precisões (intra e inter dia) revelaram um desvio padrão relativo máximo de 3,51%. O método é robusto considerando mudanças realizadas no fluxo da fase móvel, temperatura e marca da coluna. Os limites de detecção e quantificação foram de 10,86 e 32,78 ng/mL, respectivamente. O método cumpriu os requisitos para ser considerado confiável e viável para aplicação na análise quantitativa de 5-FU em nanopartículas poliméricas.Unitermos: Fluorouracila/determinação. Nanopartículas. Cromatografia líquida de alta eficiências/ análise quantitativa/validação de método.

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Section: Introductionmentioning

confidence: 99%

Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles

Mattos

Khalil

Mainardes

2013

Braz. J. Pharm. Sci.

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“…The detection limits reported at different methods for 5-FU are tabulated in the Table 2. This method was better as compared to other reported methods (Hua X. et al 2013;Sataraddi S. R. and Nandibewoor S. T. 2011;Badea I. et al 2002;Fars K.A et al 2009;Tianrong Z. et al 2011). Repeatability of the electrode renewed every time was studied for every several hours within a day, and percentage RSD has been calculated to be 1.6 %.…”

Section: Concentration Variationmentioning

confidence: 57%

Electrochemical behavior of anticancer drug 5-fluorouracil at carbon paste electrode and its analytical application

Bukkitgar

Shetti

2016

J Anal Sci Technol

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Background: A set of pyrimidine nucleobase present in all living systems as a component of nucleic acid constitutes uracil together with thymine and cytosine. A diverse physiological activity is exhibited by many N-substituted uracil derivatives. In oncology, 5-FU is widely used as an important anticancer drug. Methods: Electrochemical behavior was studied using cyclic voltammetric method, and the analytical application was studied using differential pulse voltammetric method. Solution pH has been measured by pH meter. Results: The process on the surface of electrode was found to be irreversible and diffusion controlled. The charge transfer coefficient, heterogeneous rate constant, and the number of electron transferred were calculated. Possible reaction mechanism taking place on the surface of electrode was proposed. Calibration plot constructed using differential pulse voltammetric technique was used for quantitative analysis in pharmaceutical and human urine sample. Limit of detection (LOD) and limit of quantification (LOQ) were calculated to be 12.25 and 40.8 nM, respectively. Conclusions: In the present work, we described the electrochemical behavior of anticancer drug and its determination in human urine and pharmaceutical samples. The method shows the development of a sensor for selective and sensitive determination of 5-FU.

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“…The maximum absorption peaks at 200 nm in all samples are due to the presence of PVP on the surface of the nanosheets [ 81 ]. Samples containing Tegafur show a typical absorption peak at 270 nm [ 87 , 88 ]. The NIR absorbance of the MoS 2 + Teg HG and MoS 2 HG (810 nm) increased 1.6-fold compared to the MoS 2 6 h ultrasonication water dispersions.…”

Section: Hydrogels’ Characterizationmentioning

confidence: 99%

New MoS2/Tegafur-Containing Pharmaceutical Formulations for Selective LED-Based Skin Cancer Photo-Chemotherapy

Campos,

Silva,

Fernandes

et al. 2024

Pharmaceutics

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Non-melanoma skin cancer (NMSC) is one of the most common types of cancer worldwide. Despite the low mortality rate, rising incidence and recurrence rates are a burden on healthcare systems. Standard treatments such as chemotherapy, radiotherapy, and surgery are either invasive or toxic to healthy tissues; therefore, new, alternative, selective treatments are needed. In this work, a combined photothermal and chemotherapeutic approach is proposed. MoS2 was used as photothermal agent. It was prepared by a liquid-phase exfoliation and intercalation method using polyvinylpyrrolidone (PVP), followed by recirculation through a custom-built high-power ultrasonication probe. After 6 h of ultrasonication treatment, the average particle size was 165 ± 170 nm. Near-infrared (NIR) irradiation assays (810 nm, 0.1 W/cm2, 30 min, 180 J/cm2) confirmed that MoS2 nanosheets can efficiently convert NIR light into heat and reach 52 °C. The therapeutic doses of MoS2 (125 µg/mL) and Tegafur (50 µg/mL) were optimized and both were simultaneously incorporated into a Carbopol hydrogel. The cells were brought into contact with the hydrogel and irradiated with a custom-built NIR LED system. In HFF-1 cells (normal human fibroblasts), the metabolic activity was 78% (above the 70% toxicity limit—ISO 10993-5:2009(E)), while in A-431 skin cancer cells, it was 28%. In addition, the MoS2 + Tegafur hydrogels led to a 1.9-fold decrease in A-431 cancer cell metabolic activity, 72 h after irradiation, in comparison to MoS2 hydrogels, indicating a combined effect of photothermal and chemotherapy.

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Determination of the 5-fluorouracil and N1(2′-furanidyl)uracil in the presence of tegafur by zero-crossing first derivative spectrometry

Cited by 9 publications

References 13 publications

Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles

Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles

Electrochemical behavior of anticancer drug 5-fluorouracil at carbon paste electrode and its analytical application

New MoS2/Tegafur-Containing Pharmaceutical Formulations for Selective LED-Based Skin Cancer Photo-Chemotherapy

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