Determination of the amount of insecticide picked up by mosquitoes from treated nets

Abstract

Background: Insecticides used in vector control mostly rely on vectors being exposed through contact with treated surfaces, yet little is known about the amount picked up by the insect. Measuring this amount is relevant not only for determining the actual doses that are lethal to the mosquito, but also for understanding effects on the physiology and vector competence of mosquitoes. Insecticides at sub-lethal doses can affect both parasites developing inside mosquitoes and mosquito microbiota, hence it is important to understand the processes by which parasites are exposed to insecticide inside the insect. These doses will inevitably depend on the amount of insecticide that mosquitoes pick up when they come into contact with treated nets. Methods: Three to five days old non-blood fed female Anopheles coluzzii mosquitoes were exposed to a long-lasting insecticidal net (PermaNet 2.0 containing 55 mg/m2 deltamethrin), using a wire ball frame, for 0.5-5.0 minutes. Our in-house developed colorimetric test was used to visually detect the amount of deltamethrin on different parts of the mosquito (legs, heads, thoraxes, abdomens) following exposure to the net. The amount of insecticide picked up by mosquitoes from the net over a range of exposure times was measured using a high-performance liquid chromatography with diode array detection (HPLC-DAD). Results: The colorimetric test, designed to only detect the type 2 pyrethroids (i.e deltamethrin, α-cypermethrin and λ-cyhalothrin) on fabrics (e.g. ITNs) and sprayed walls, was successfully used for the first time to detect deltamethrin on mosquitoes following exposure to the net. The confirmatory HPLC-DAD analysis determined that after 2 min exposure up to 12 ng of deltamethrin adhered to mosquitoes following exposure to PermaNet 2.0 (mean = 5.2 ng/mosquito, SE = 1.9) and that the final dose depends on the length of exposure time. Conclusions: This study demonstrated the potential of a screening (type 2 pyrethroid colorimetric test) and a confirmatory test (HPLC-DAD) to determine the amount of insecticide that adheres to mosquitoes on contact with treated surfaces. This has implications for a precise lethal dose determination and detection of specific insecticide that causes the greatest mosquito mortality in circumstances where mixtures of insecticides may be used to maximise effectiveness of interventions.