Determination of the anti-inflammatory agent carprofen, (d,l)-6-chloro-α-methylcarbazole-2-actic acid, in blood by high-pressure liquid chromatography

Puglisi, Carl V.; Meyer, John C.; Silva, J.Arthur F. de

doi:10.1016/s0021-9673(00)95197-5

Cited by 15 publications

(2 citation statements)

References 3 publications

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“…Carprofen has also been shown to be a modulator of canine chondrocyte metabolism, where doses > 20 μg mL −1 ( in vitro in the cell media) suppress chondrocyte function, and a carprofen concentration of 1 and 10 μg mL −1 had a potentially beneficial effect on cartilage matrix maintenance 17 . Human synovial and serum concentrations correlate well, and a single oral dose of 100 mg results in 3.5–9.7 μg mL −1 in the synovial fluid 27 , 28 . Using orthopedic and surgical pain models, analgesia from carprofen treatment has been documented in humans, 18 dogs, 16 cats, 29 horses, 30 and rats, 31 and has been shown to result from the anti‐inflammatory effects of the drug 14 .…”

Section: Discussionmentioning

confidence: 99%

Carprofen inhibition of flare in the dog measured by laser flare photometry

Krohne

Blair

Bingaman

et al. 1998

Veterinary Ophthalmology

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The purpose of this study was to determine whether oral carprofen (Rimadyl(R)) treatment in dogs could prevent or decrease the breakdown of the blood-aqueous barrier. The topical pilocarpine irritative model was used to induce breakdown and cause flare. Pilocarpine was instilled in both eyes of seven dogs at time zero and again 5 h later. At 7 h, laser flare photometry was used to measure the flare concentration in each eye using the Kowa FC-1000 laser flare cell meter. All treatments were then discontinued. Two days later, carprofen was administered to the same dogs for a total of three doses. After the last dose of carprofen, pilocarpine treatments and flare measurements were repeated. Carprofen pretreatment resulted in a 68% inhibition of flare, which was highly significant (P < 0.01). The pilocarpine group had a mean of 16.1 photon counts per millisecond (PC ms-1) +/- 2.2 SE, and the carprofen group had a mean of 7.0 PC/ms +/- 1.2 SE. These results compare favorably with previous studies measuring increased protein or fluorescein concentrations in the aqueous humor after blood-aqueous barrier breakdown in the dog. These results suggest that carprofen may be effectively used as a systemically administered ocular anti-inflammatory drug. Carprofen has the added benefit of fewer reported side effects.

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Section: Discussionmentioning

confidence: 99%

Carprofen inhibition of flare in the dog measured by laser flare photometry

Krohne

Blair

Bingaman

et al. 1998

Veterinary Ophthalmology

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“…A number of different methods have been published lately for the individual determination of several of the marketed non-steroidal weak analgesic and antiinflammatory drugs in serum or plasma (Anttila 1977;Pitre & Grandi 1979;Puglisi et a/. 1977;Stenberg et al 1979;Lo & Bye 1979;Chatfield & Woodage 1978;Plakogiannis & Saad 1978;Farinotti & Mahuzier 1979;Munson et al 1978;Lear er al.…”

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confidence: 99%

HPLC‐Determination of some Antiinflammatory, Weak Analgesic and Uricosuric Drugs in Human Blood Plasma and its Application to Pharmacokinetics

Nielsen‐Kudsk

1980

Acta Pharmacologica et Toxicologica

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HPLC-determination of naproxen, indomethacin, ketoprofen, fenoprofen, ibuprofen, diclofenac sodium, tolfenamic acid, phenylbutazone, mofebutazone, salicylic acid, acetylsalicylic acid, phenacetin, paracetamol, sulfinpyrazone and probenecid by means of an adjustable, rapid, accurate and specific method is described. Plasma samples of 0.2 ml were deproteinised and the drugs extracted simultaneously with pure acetonitrile. Aliquots of 25 p1 of this primary extract were directly injected on the column. As elution solvent for drug screening was basically used 55% methanol in 50 mMphosphate buffer at pH 4.0. Optimal separation of some drugs or reasonable elution times for others were obtained by varying the methanol concentration of the elution solvent or possibly its pH. The method used for individual drug determinations is very applicable for therapeutic monitoring purposes as well as for use in pharmacokinetic investigations. As an example, the practical detection limit for naproxen in plasma was about 0.2 pg ml-'. By concentrating the extract this could be lowered to about 0.04 pg ml-'. The method was applied in a study of the pharmacokinetics of naproxen in a person, who ingested a single oral dose of 2.5 mg kg-'. Pronounced two-compartment kinetics were found. V, was 0.038 1 kg-', Vdrs 0.138 1 kg-l, t % (p) 21.3 hrs, ty2 (a) 0.99 hr and ty2 (k.) 0.67 hr.

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Determination of carprofen in blood by gas chromatography chemical ionization mass spectrometry

Hodshon

Garland

Perry

et al. 1979

Biol. Mass Spectrom.

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A gas chromatographic chemical ionization mass spectrometric assay has been developed to measure carprofen in blood. The method features the addition of either a structural or stable isotope analog internal standard to plasma prior to a simple benzene extraction at pH 4.5. The residue, after removal of the benzene, is methylated with ethereal diazomethane. Following evaporation of the methylating solvents, a portion of the reconstituted residue is analyzed by gas chromatography mass spectrometry. The mass spectrometer is set to monitor in the gas chromatographic effluent the [MH]+ ions of carprofen methyl ester and the methyl ester of the internal standard generated by isobutane chemical ionization. Assay sensitivity is 5 pmol ml-1. When 200 pmol ml-1 samples are analyzed using a stable isotope analog as the internal standard, the precision and accuracy are both 4%. Using a structural analog as the internal standard, the assay was neither as precise nor as accurate.

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Determination of the anti-inflammatory agent carprofen, (d,l)-6-chloro-α-methylcarbazole-2-actic acid, in blood by high-pressure liquid chromatography

Cited by 15 publications

References 3 publications

Carprofen inhibition of flare in the dog measured by laser flare photometry

Carprofen inhibition of flare in the dog measured by laser flare photometry

HPLC‐Determination of some Antiinflammatory, Weak Analgesic and Uricosuric Drugs in Human Blood Plasma and its Application to Pharmacokinetics

Determination of carprofen in blood by gas chromatography chemical ionization mass spectrometry

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