Determination of the antigenic properties of quinoidr adiotoxin using the indirect hemagglutination test (IHA)

Abstract

Relevance. The article presents the results of experiments to determine the antigenic properties (the ability of quinoid radiotoxin to synthesize antiradiotoxic antibodies) of plant radiotoxin using IHA.Methods. An antigenic variant of the erythrocyte diagnosticum (AGED), which is a ram erythrocytes sensitized by radiation antigens, was used to conduct the IHA-test. As control (positive) antiradiation antibodies in the reaction, we used antiradiation antibodies obtained by hyperimmunization of rabbits with a radiation antigen — radiotoxin isolated from the liver tissue of lethally irradiated sheep, tested antitoxic antibodies — hyperimmune sera from sheep hyperimmunized with quinoid (plant) radiotoxin, negative serum obtained from intact (non-irradiated, not vaccinated with any vaccines) sheep, heterologous sera (antiburn, anticolibacillary). The reaction was placed on microplates in a volume of 50 ml and was taken into account according to a four-point system in crosses.Results. The study of the antigenic activity of the test radiation antigen — plant quinoidr adiotoxin (QRT) — using the IHA-test showed that the test antigen has a high antigenic activity, inducing in the body of immunized animals (sheep) the synthesis of antiradiotoxic antibodies, the titers of which significantly exceed those immunized with liver (animal) antigen — animal radiotoxin. Quinoidr adiotoxin, obtained from plant tissue (potato tubers) and conjugated with a protein-lipoid group (incomplete Freund's adjuvant — IFA), has a high antigenicity, inducing the synthesis of specific antiradiotoxic antibodies, which can be used as an essential component of the immunochemical test system — sensitin used for sensitization of micro- and nanoparticles of bentonite in the design of antiradiation antibody variant of bentonited iagnosticum (ABBD).