Determination of the Antioxidant Capacity of Red Fruits by Miniaturized Spectrophotometry Assays

Becker, Magda Márcia; Nunes, Gilvanda Silva; Ribeiro, Danilo Braga; Silva, Francisco E. P. S.; Catanante, Gaëlle; Marty, Jean‐Louis

doi:10.21577/0103-5053.20190003

Cited by 37 publications

(34 citation statements)

References 18 publications

(34 reference statements)

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“…A previous study on radical scavenging activity demonstrated high antioxidant activity of H. cupressiforme methanolic extract [ 6 ]. This discrepancy in the obtained results could be explained by different extraction solvent and extraction procedure overall, different radical final concentration, or contrasting ecological conditions of the growth site such as season and climate [ 20 ]. For instance, it has been shown that the content of secondary metabolites in mosses depends on the season, with the greatest number of biochemical features manifested in the summer [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.

et al. 2020

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Recently, there has been an increasing interest in the chemistry and biological potential of mosses, since a large number of biologically active compounds have been found within these species. This study aimed at examining the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory/antineurodegenerative, and antitumor activities) of moss Hypnum cupressiforme Hedw. extracts. Corresponding extracts have been obtained applying Soxhlet extractor. The chemical characterization was performed using spectrophotometric assays and liquid chromatography–mass spectrometry (LC-MS). The extracts were analyzed for antioxidant activity and for inhibitory activities on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase. Additionally, extracts were tested against four cell lines—MRC-5, BV2, HCT-116, and MDA-MB-231—for antitumor and anti-inflammatory activities. Chemical analysis of extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. Major compounds identified by LC-MS in H. cupressiforme were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. According to biochemical assays the investigated extracts exhibited significant immunomodulatory potential. Significant antiproliferative potential against MDA-MB-231 cells has been observed together with the promising anti-neuroinflammatory application. The obtained data suggest that moss H. cupressiforme is a valuable natural source of biologically active compounds with potential application in the pharmaceutical industry.

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Section: Discussionmentioning

confidence: 99%

Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.

et al. 2020

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“…When applying the DPPH assay, the results are similar, which was demonstrated by Šekularac et al [ 27 ], who found very slight, if any activity of isatin against DPP • radical. The centre of radical in DPP • probe is localized mostly within nitrogen atom [ 28 ] and the intense purple color of DPP • radical on abstracting an atom of hydrogen (H • ) alters to yellow [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Oxidative Degradation of High-Molar-Mass Hyaluronan: Effects of Some Indole Derivatives to Hyaluronan Decay

Valachová

Mach

Šoltés

2020

IJMS

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Indole derivatives such as isatin (a natural compound), cemtirestat, stobadine, and its derivatives (synthetic compounds) are known to have numerous positive effects on human health due to regulation of oxidative status. The aim of the study was to assess radical scavenging capacities of these compounds and explore their potential protective effects against reactive oxygen species formed during Cu(II) ions and ascorbate-induced degradation of high-molar-mass hyaluronan. Based on the IC50 values determined by the ABTS assay, the most effective compound was SM1M3EC2·HCl reaching the value ≈ 11 µmol/L. The lowest IC50 value reached in the DPPH assay was reported for cemtirestat ≈ 3 µmol/L. Great potency of inhibition of hyaluronan degradation was shown by cemtirestat, followed by isatin even at low concentration 10 µmol/L. On the other hand, stobadine·2HCl had also a protective effect on hyaluronan degradation, however at greater concentrations compared to cemtirestat or isatin. SME1i-ProC2·HCl reported to be a less effective compound and SM1M3EC2·HCl can be considered almost ineffective compared to stobadine·2HCl. In conclusion, our results showed that both isatin and cemtirestat were capable of attenuating the degradation of high-molar-mass hyaluronan due to their ability to complex/sequester cupric ions.

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“…These three assays considered as relatively inexpensiveness spectrophotometric-based tests based on color quenching or gaining of synthetic organic radicals. The colors of DPPH (purple) and ABTS (blue–green) are reducible upon exposure to antioxidants, and this decrease is negatively correlated with the measured wavelength, which, thus, refers to the antioxidant capacity [ 61 ]. While the FARP assay works in reverse; the increase in color density (decrease in wavelength) refers to higher antioxidant capacity.…”

Section: Stingless Bee Honeymentioning

confidence: 99%

Antioxidant-Based Medicinal Properties of Stingless Bee Products: Recent Progress and Future Directions

et al. 2020

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Stingless bees are a type of honey producers that commonly live in tropical countries. Their use for honey is being abandoned due to its limited production. However, the recent improvements in stingless bee honey production, particularly in South East Asia, have brought stingless bee products back into the picture. Although there are many stingless bee species that produce a wide spread of products, known since old eras in traditional medicine, the modern medical community is still missing more investigational studies on stingless bee products. Whereas comprehensive studies in the current era attest to the biological and medicinal properties of honeybee (Apis mellifera) products, the properties of stingless bee products are less known. This review highlights for the first time the medicinal benefits of stingless bee products (honey, propolis, pollen and cerumen), recent investigations and promising future directions. This review emphasizes the potential antioxidant properties of these products that in turn play a vital role in preventing and treating diseases associated with oxidative stress, microbial infections and inflammatory disorders. Summarizing all these data and insights in one manuscript may increase the commercial value of stingless bee products as a food ingredient. This review will also highlight the utility of stingless bee products in the context of medicinal and therapeutic properties, some of which are yet to be discovered.

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Determination of the Antioxidant Capacity of Red Fruits by Miniaturized Spectrophotometry Assays

Cited by 37 publications

References 18 publications

Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.

Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.

Oxidative Degradation of High-Molar-Mass Hyaluronan: Effects of Some Indole Derivatives to Hyaluronan Decay

Antioxidant-Based Medicinal Properties of Stingless Bee Products: Recent Progress and Future Directions

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