Determination of the association of <i>GHR</i>/<i>AluI</i> gene polymorphisms with milk yield traits in Holstein and Jersey cattle raised in Turkey

Abstract

Abstract. This research was carried out to determine the effect of a specific single nucleotide polymorphism (SNP) region in exon 10 of the growth hormone receptor (GHR) gene on milk production traits in Jersey and Holstein cows raised in Turkey. Milk samples were recorded as a test day milk yield (TDMY) and an adjusted based 305 d milk yield (305-DMY). Also, milk component traits were detected. Based on the scope of this study, a total of 748 dairy cows, including 305 Holsteins raised in the Marmara Region and 163 Holstein and 280 Jersey raised in the Black Sea Region, were genotyped for the GHR gene using the RFLP-PCR technique. Jersey cows carrying the GG genotype (5.24 %) were associated with higher fat content (P<0.05). Jersey cows with GG and AG also had a higher protein content (3.44 % and 3.38 %, respectively) (P<0.05). Similarly, the protein content was the highest in Holstein cows with the GG genotype (3.46 %) (P<0.01), whereas Holstein cows having AA genotypes displayed higher TDMY (24.64 kg/d) (P<0.05) and 305-DMY (8472.4 kg) (P<0.01). The estimated increase in milk protein and fat contents due to the G allele was 0.07 % and 0.22 % in the Jersey breed, respectively. On the other hand, allele A was highly related to an increase in protein yield and 305-DMY of 0.04 and about 675 kg in the Holstein breed, respectively. The GHR gene should be considered as a potential candidate gene in marker-assisted selection programs to improve the performance of milk and related traits in Turkey dairy cattle populations.

DNA methylation of GDF-9 and GHR genes as epigenetic regulator of milk production in Egyptian Zaraibi goat

“…GHR and GDF-9 genes play a crucial role during lactation. GHR gene and its polymorphisms have a vital role in the development of the mammary gland and milk production (El-Komy et al 2020 ; Nanaei et al 2020 ; Cobanoglu et al 2021 ; Erdoğan et al 2021 ). Several studies indicated that GDF-9 increases ovarian follicles growth, and ovulation rate, and its polymorphisms had a significant correlation with litter size, number of lambs, twining %, and thus milk production (Al-Khuzai and Ahmed 2019 ; Koyun, et al 2021 ).…”

Overview of SNPs Associated with Trans Fat Content in Cow’s Milk

“…The growth hormone receptor (GHR) is involved in the regulation of postnatal growth of the body. Polymorphism in the GHR gene showed an association with milk yield traits and composition in Turkish Holstein, Turkish Jersey, and Chinese Holstein cattle [56,57]. The p.Phe279Tyr mutation in the GHR was associated with the protein percentage in the Chinese dairy population (p = 0.0014) [58].…”