Determination of the Bioadhesion Indicators of Vaginal Gel With Resveratrol and Hyaluronic Acid

Abstract

Aim. The purpose of the work is to determine the bioadhesion indices of vaginal gel with resveratrol and hyaluronic acid, as well as the choice of the type and content of mucoadhesives in the composition. Materials and methods. As research objects samples of gels with different mucoadhesives in the composition were used. Among used mucoadhesives were: sodium alginate (FMC BioPolimer AS, Norway), methyl cellulose (Shin Etsu, Germany), Methocel – methyl cellulose with hydroxypropylmethyl cellulose (Dow Pharmaceutical Sciences, USA), OraRez® W-100L16 – vinyl methyl ether and maleic anhydride copolymer (BOAI, China). As a comparison drug, vaginal gel "Gynodec" (Yuriya-Pharm) was used. During the study, the rate of gel distribution, the degree of deformation under the influence of mechanical forces, the degree of the gel fixation on the surface of the mucosa and the adhesion ability of the samples have been determined. Results. The study has determined that sample No. 2 with sodium alginate has the highest distribution rate, which was 1.56 cm/min. The study of the fixation of samples on the surface of the model of the mucous was performed by the method of flow. The results have showed that the sample with sodium alginate has the closest value to the reference drug. The adhesive ability of samples with different sodium alginate contents was determined. The tensimetric study has found that at a concentration of 0.5 %, the force required to separate the surface is 6158 Pa. Conclusions. On the basis of the complex of physico-chemical studies, bioadhesion indicators of vaginal gel with resveratrol, depending on the type and concentration of mucoadhesives have been determined. According to the distribution parameters on the surface of the genital mucosa model, it has been found that the best properties compared with other types of mucoadhesives has a sample containing sodium alginate. The study by means of a strain gauge has found that the addition of sodium alginate at a concentration of 0.5 % would provide a satisfactory adhesive ability of the vaginal gel.

Development, Optimization, and In Vitro Evaluation of Novel Oral Long-Acting Resveratrol Nanocomposite In-Situ Gelling Film in the Treatment of Colorectal Cancer

“…Using the pH-profile drug release, the amounts of NA and Sp and film preparation method were optimized to achieve a minimum release in the stomach-simulated media and sustained-release (SR) behavior in the intestinal and colonic media. The SR system of the pH-selective release was aimed to decrease the metabolism associated with the free drug and to decrease the number of daily doses to enhance the efficiency associated with the Rv use as anti-inflammatory or anticancer treatments [ 15 , 16 , 17 , 18 , 19 , 20 ].…”

“…The room temperature drying was used to avoid the Rv degradation and Sp hardening after oven drying [ 12 , 15 ]. The fume-hood was closed, and the air suctioning was accelerating the water evaporation for 12 h. The weight of the granules was measured after the optimization for the procedure and time to have a final dry granules weight equivalent to the added Sp and Rv weights ± 0.02 g. These films were intended to be given orally as a dry film to be given with 15 mL of water, coded as NA-Sp-Rv-600 and NA-Sp-Rv-1200 [ 13 , 14 , 16 ].…”

“…The most optimum formula (NA-Sp-Rv-1200) was stored at room temperature in an amber glass bottle containing a desiccant bag to decrease the film’s water uptake during storage. The drug release experiments for the formula were conducted after 2-, 4- and 8-weeks of storage [ 16 ].…”

Modern integrated approach in the treatment of atrophic vaginitis in women of menopausal age

“…• клінічних даних: сухість, свербіж, дискомфорт у піхві, біль під час статевого акту, рецидивні вагінальні й контактні кров'янисті виділення; • виявлення кольпоскопічних ознак атрофічного процесу: нерівний рельєф, червоний колір слизової оболонки з гіпертрофією запалених судин, що кровоточать у разі дотику, субатрофічні крововиливи, вторинне інфікування з гіперемією піхви, сірим нальотом і гнійними виділеннями [19]. Для визначення мікробіоценозу використовували критерій Ньюджента (бали від 0 до 10), який ґрунтується на результатах мікроскопії мазка та визначенні співвідношення морфотипів Lactobacillus, G.vaginalis і Mobiluncus.…”