Determination of the biological variability of porcine explant tissue in retinal damage ex-vivo experiments with optical laser radiation

Abstract

The emission limits and the rulesets in the international laser safety standard IEC 60825-1:2014 are based on scientific evidence from damage threshold experiments and simulations. However, available data sets of laser-induced damage experiments for specific parameter spaces are limited. Therefore, further determination of damage thresholds for laser systems, operating in these parameter spaces, is crucial. Retinal damage thresholds are determined with experiments using animal tissue and optical setups with pulsed lasers and beam profiles of constant irradiance. Several factors influencing these experiments, such as the focusing were identified. This work investigates the biological variability of porcine tissue samples by minimizing the influencing factors and performing damage experiments.