Determination of the Brain–Blood Partition Coefficient for Water in Mice Using MRI

Leithner, Christoph; Müller, Susanne; Füchtemeier, Martina; Lindauer, Ute; Dirnagl, Ulrich; Royl, Georg

doi:10.1038/jcbfm.2010.160

Cited by 42 publications

(49 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The animals were anesthetized with isoflurane administered through a facemask and adjusted to maintain respiratory rate in the physiologic range following published protocols. 27 Body temperature was maintained at 37°C using a heading pad. A 30-cm horizontal bore Bruker Biospin magnet operating at 7 T (Bruker BioSpin GmbH, Karlsruhe, Germany) was used with a S116 gradient set.…”

Section: Arterial Spin Labeling Magnetic Resonance Imagingmentioning

confidence: 99%

Pharmacologically-Induced Neurovascular Uncoupling is Associated with Cognitive Impairment in Mice

Tarantini

Hertelendy

Tucsek

et al. 2015

J Cereb Blood Flow Metab

115

144

View full text Add to dashboard Cite

There is increasing evidence that vascular risk factors, including aging, hypertension, diabetes mellitus, and obesity, promote cognitive impairment; however, the underlying mechanisms remain obscure. Cerebral blood flow (CBF) is adjusted to neuronal activity via neurovascular coupling (NVC) and this mechanism is known to be impaired in the aforementioned pathophysiologic conditions. To establish a direct relationship between impaired NVC and cognitive decline, we induced neurovascular uncoupling pharmacologically in mice by inhibiting the synthesis of vasodilator mediators involved in NVC. Treatment of mice with the epoxygenase inhibitor N-(methylsulfonyl)-2-(2-propynyloxy)-benzenehexanamide (MSPPOH), the NO synthase inhibitor L-NGNitroarginine methyl ester (L-NAME), and the COX inhibitor indomethacin decreased NVC by over 60% mimicking the aging phenotype, which was associated with significantly impaired spatial working memory (Y-maze), recognition memory (Novel object recognition), and impairment in motor coordination (Rotarod). Blood pressure (tail cuff) and basal cerebral perfusion (arterial spin labeling perfusion MRI) were unaffected. Thus, selective experimental disruption of NVC is associated with significant impairment of cognitive and sensorimotor function, recapitulating neurologic symptoms and signs observed in brain aging and pathophysiologic conditions associated with accelerated cerebromicrovascular aging.

show abstract

Section: Arterial Spin Labeling Magnetic Resonance Imagingmentioning

confidence: 99%

Pharmacologically-Induced Neurovascular Uncoupling is Associated with Cognitive Impairment in Mice

Tarantini

Hertelendy

Tucsek

et al. 2015

J Cereb Blood Flow Metab

115

144

View full text Add to dashboard Cite

show abstract

“…where ∆M(t) is the tissue magnetization difference between the control and label images during the interval t, M 0B is the equilibrium magnetization of arterial blood assumed to be the same as the tissue M0 obtained from the T1 mapping, α is the labeling efficiency assumed to be 0.9, λ is the tissue/blood partition coefficient of water assumed to be 0.9ml/g [31], ∆t is arterial transit time to be fitted, τ is the labeling duration (1600ms in our sequence), T' 1 is the apparent relaxation time measured by the T 1 mapping, T 1b is the longitudinal relaxation time of arterial blood assumed to be 2300ms [32]. The goodness of fitting for CBF quantification was evaluated by the coefficient of determination, R 2 .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of EPI distortion correction methods for quantitative MRI of the brain at high magnetic field

Hong

Teh

et al. 2015

Magnetic Resonance Imaging

View full text Add to dashboard Cite

“…The degree of the inversion efficiency was assumed to be alpha = 0.7 [19], [20]. In close approximation to the value recently reported by Leithner et al for the mouse brain [21] the brain-blood partition coefficient value for water was assumed to be lambda = 0.90 mL/g. Slice selective T1 mapping was measured with a single slice partial saturation inversion recovery RARE sequence (TI of 0.02 s, 0.5 s, 1.0 s, 1.5 s, 2.0 s, 3.0 s, 5.0 s, 10.0 s).…”

Section: Methodsmentioning

confidence: 96%

Sustained Reperfusion after Blockade of Glycoprotein-Receptor-Ib in Focal Cerebral Ischemia: An MRI Study at 17.6 Tesla

et al. 2011

View full text Add to dashboard Cite

BackgroundInhibition of early platelet adhesion by blockade of glycoprotein-IB (GPIb) protects mice from ischemic stroke. To elucidate underlying mechanisms in-vivo, infarct development was followed by ultra-high field MRI at 17.6 Tesla.MethodsCerebral infarction was induced by transient-middle-cerebral-artery-occlusion (tMCAO) for 1 hour in C57/BL6 control mice (N = 10) and mice treated with 100 µg Fab-fragments of the GPIb blocking antibody p0p/B 1 h after tMCAO (N = 10). To control for the effect of reperfusion, additional mice underwent permanent occlusion and received anti-GPIb treatment (N = 6; pMCAO) or remained without treatment (N = 3; pMCAO). MRI 2 h and 24 h after MCAO measured cerebral-blood-flow (CBF) by continuous arterial-spin labelling, the apparent-diffusion-coefficient (ADC), quantitative-T2 and T2-weighted imaging. All images were registered to a standard mouse brain MRI atlas and statistically analysed voxel-wise, and by cortico-subcortical ROI analysis.Results Anti-GPIb treatment led to a relative increase of postischemic CBF vs. controls in the cortical territory of the MCA (2 h: 44.2±6.9 ml/100 g/min versus 24 h: 60.5±8.4; p = 0.0012, F(1,18) = 14.63) after tMCAO. Subcortical CBF 2 h after tMCAO was higher in anti-GPIb treated animals (45.3±5.9 vs. controls: 33.6±4.3; p = 0.04). In both regions, CBF findings were clearly related to a lower probability of infarction (Cortex/Subcortex of treated group: 35%/65% vs. controls: 95%/100%) and improved quantitative-T2 and ADC. After pMCAO, anti-GPIb treated mice developed similar infarcts preceded by severe irreversible hypoperfusion as controls after tMCAO indicating dependency of stroke protection on reperfusion.ConclusionBlockade of platelet adhesion by anti-GPIb-Fab-fragments results in substantially improved CBF early during reperfusion. This finding was in exact spatial correspondence with the prevention of cerebral infarction and indicates in-vivo an increased patency of the microcirculation. Thus, progression of infarction during early ischemia and reperfusion can be mitigated by anti-platelet treatment.

show abstract

Determination of the Brain–Blood Partition Coefficient for Water in Mice Using MRI

Cited by 42 publications

References 18 publications

Pharmacologically-Induced Neurovascular Uncoupling is Associated with Cognitive Impairment in Mice

Pharmacologically-Induced Neurovascular Uncoupling is Associated with Cognitive Impairment in Mice

Evaluation of EPI distortion correction methods for quantitative MRI of the brain at high magnetic field

Sustained Reperfusion after Blockade of Glycoprotein-Receptor-Ib in Focal Cerebral Ischemia: An MRI Study at 17.6 Tesla

Contact Info

Product

Resources

About