Determination of the cephalosporin Ro 13‐9904¹ in plasma, urine, and bile by means of ion‐pair reversed phase chromatography

Abstract

An HPLC method has been developed for the determination of the cephalosporin antibiotic Ro 13-9904 in plasma, urine, and bile of dogs and of human volunteers using the technique of ionpairchromatographywith a LiChrosorbRP-18 column.The three mobile phases employed contained tetrapentyl-, tetraoctyl-and hexadecyltrimethyl-ammonium bromide, respectively, as lipophilic counterions. The chromatographic conditions chosen allowed simple and rapid sample preparation. Plasma was deproteinired with ethanol and the supernatant was directly injected onto the column; urine and bile were diluted with mobile phase and injected without any purification. The detection limit for the cephalosporin was about 0.5 pg/ml for plasma samples and approximately 5 pg/ml for bile and urine.

Randomized Treatment of Patients with Typhoid Fever by Using Ceftriaxone or Chloramphenicol

“…Blood was obtained for complete blood counts and for determining concentrations of creatinine and electrolytes. Serum concentrations of ceftriaxone and chloramphenicol were determined by high-performance liquid chromatography [9,10] on specimens taken 24 h after a dose of ceftriaxone and 6 h after a dose of chloramphenicol on days 3 and 7 of treatment. Cultures of blood were repeated on days 3 and 14.…”

Simultaneous Determination of Ceftriaxone Sodium and Statin Drugs in Pharmaceutical Formulations and Human Serum by Rp-HPLC

“…There are a number of liquid chromatographic methods reported in the literature for the individual assays of ceftriaxone in aqueous and biological sample [24][25][26][27][28][29]. Owens achieved chromatographic separation by using cetyltrimethylammonium bromide (0.01 M) as ion-pairing agent.…”

No effect of probenecid on the renal and biliary clearances of digoxin in man.

“…method, originally developed for analysis of ceftriaxone (Trautmann & Haefelfinger, 1981). Duplicate determinations of quality control samples analysed concomitantly with the plasma samples resulted in inter-assay variations of 3.4% (170 ,umol 1-1) and 7.3% (424 pxmol 1-1), and the intra-assay variations of determinations on spiked plasma samples were 0.8% (78 ,umol 1-1; n = 6) and 3.0%…”