Determination of the Chronic Toxicity Parameters of the Complex Remedy for the Treatment of Cysticercosis in Rabbits

Abstract

The article presents the data of a preclinical test of a means for the treatment of cysticercosis in rabbits by intra-gastric administration to white mice. Cysticercus pisiformis is a disease caused by ingestion of eggs of Taenia pisiformis in food or water, a tapeworm that parasitizes the small intestine of dogs and other carnivores. As a result of the invasion, the body loses its ability to fully function, the resistance of the immune system to infectious diseases decreases. In order to avoid the spread of the disease, it is necessary to make a timely diagnosis. The obtained data can be used to take the necessary measures against infestations and are important to prevent the selective use of anti-parasitic agents, which can lead to anthelmintic resistance.
 The purpose of the work was to determine the parameters of chronic toxicity of the developed complex anti-parasitic agent for the treatment of rabbits for cysticercosis on white mice under experimental conditions.
 The animals of the experimental groups were given an aqueous mixture of 0.0002 dm3 of the drug containing the following doses of the drug daily for 12 days: 1/100 DL50 ‒ 16.80 mg/kg (I group); 1/50 DL50 ‒ 33.58 mg/kg (II group); 1/25 DL50 ‒ 67.18 mg/kg (III group). On the next day after the last administration of the drug, on mice, under the condition of light ether narcosis, blood samples were taken for morphological and biochemical studies.
 The agent for the treatment of cysticercosis in rabbits, at doses of 1/50 DL50 and 1/25 DL50, resulted in a slight decrease in hemoglobin content and erythrocyte count compared to the control, but it did not suppress bone marrow erythropoiesis in laboratory animals. A decrease in the number of lymphocytes and a slight increase in the level of neutrophils were recorded, which indicates the irritating effect of increased doses of the active substance of the drug. According to the classification of chemical substances by degree of danger (DST 12.1.007–76), it belongs to the class of "moderately toxic substances".