Determination of the Circulating Red Cell Volume in Man by Radioactive Chromium 1

Kinetics of movement of iodide, sucrose, inulin and radio‐iodinated serum albumin in the central nervous system and cerebrospinal fluid of the rat

“…injection on the distribution of a tracer in the brain, an attempt was made to quantify these possible sources of error. To determine the quantity of blood remaining in the tissues, 51Cr-chromate-labelled erythrocytes and 13II-labelled serum albumin were used according to the method of Sterling & Gray (1950). To assess the effect of the I.c.…”

The blood—brain barrier and the extracellular space of brain

“…Since the amount of blood remaining in the brain might influence the apparent amount of substance penetrating, some experiments were carried out to estimate the average amount left. Blood cells were labelled with 51Cr according to the method of Sterling & Gray (1950) and the amount remaining in the brain after decapitation was determined.…”

The Use of Radioactive Chromium 51 as an Erythrocyte Tagging Agent for the Determination of Red Cell Survival in Vivo 12

“…Gray and Sterling (9,10) have demonstrated that a firm union between erythrocytes and chromium can be established in vitro with great rapidity and with no apparent damage to the exposed cells. These observations imply that radioactive chromium might serve admirably, not only as a tagging device for measuring circulating red cell volume as proposed by these authors, but as a basis for measuring red cell survival in vivo.…”

“…The rate at which chromium combined with red cells when sodium chromate was added to whole ACD blood was dependent upon the temperature and pH of the mixture, as demonstrated in Figure 1 and Table III. At 390 C., 90 per cent of available chromium was incorporated in the erythrocytes within a period of five minutes, as compared to a 50 to 75 per cent uptake at 260 C. and 10 t4 20 per cent at 1.80 C. After thirty minutes of equilibration the proportions taken up at 26°and 10 Powdered grouping serum, Lederle Laboratories, Pearl River, New York. 390 C. were comparable, i.e., approximately 90 per cent.…”