Determination of the Distance Between the Cytochrome and Dehydrogenase Domains of Immobilized Cellobiose Dehydrogenase by Using Surface Plasmon Resonance with a Center of Mass Based Model

Tuoriniemi, Jani; Gorton, Lo; Ludwig, Roland; Safina, Gulnara

doi:10.1021/acs.analchem.9b04490

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…Nevertheless, SPR is a well-established sensing technique, the gold standard for biomolecular interaction analysis. In a step forward, proof of concept reports highlight the potential sensitivity of SPR assays to conformational changes [13,14] undergone by affinity ligands decorating the sensing chips. SPR was used in the evaluation of conformational changes of sensitive layers (proteins, polymers) in response to modifications of the parameters of the surrounding media (e.g., pH, ionic strength, etc.)…”

Section: Spr In Sensing In Particular For Conformational Changesmentioning

confidence: 99%

“…or for small analyte detection. Studies indicate that among novel SPR-powered sensing avenues: (a) the detection of low molecular analytes via refractive index changes due to analyte-ligand interaction within the sensing layer [14][15][16], and (b) it is possible to evaluate the presence and concentration of ions in solutions by measuring the conformational changes in a sensitive protein [13,17]. The influences of the physicochemical properties of the buffer media were analysed to investigate the alterations induced by the pH of solution to the functional layers of SPR sensing chips as well as to the immobilized protein [18].…”

Section: Spr In Sensing In Particular For Conformational Changesmentioning

confidence: 99%

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Real Time SPR Assessment of the Structural Changes of Adaptive Dynamic Constitutional Frameworks as a New Route for Sensing

et al. 2022

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Cross linked gold-dynamic constitutional frameworks (DCFs) are functional materials of potential relevance for biosensing applications, given their adaptivity and high responsivity against various external stimuli (such as pH, temperature) or specific interactions with biomolecules (enzymes or DNA) via internal constitutional dynamics. However, characterization and assessment of their dynamic conformational changes in response to external stimuli has never been reported. This study proves the capability of Surface Plasmon Resonance (SPR) assays to analyse the adaptive structural modulation of a functional matrix encompassing 3D gold-dynamic constitutional frameworks (Au-DCFs) when exposed to pH variations, as external stimuli. We analyse Au-DCFs formed from Au nanoparticles, (AuNP) connected through constitutionally dynamic polymers, dynamers, with multiple functionalities. For increased generality of this proof-of-concept assay, Au-DCFs, involving DCFs designed from 1,3,5-benzene-tricarbaldehyde (BTA) connecting centres and polyethylene glycol (PEG) connectors, are covalently attached to standard SPR sensing chips (Au nanolayers, carboxyl terminated or with carboxymethyl dextran, CMD top-layer) and analysed using state-of-the art SPR instrumentation. The SPR effects of the distance from the Au-DCFs matrix to the Au nanolayer of the sensing chip, as well as of Au-DCFs thickness were investigated. This study reveals the SPR response, augmented by the AuNP, to the conformational change, i.e., shrinkage, of the dynamer and AuNP matrix when decreasing the pH, and provides an unexplored insight into the sensing applicability of SPR real-time analysis of adaptive functional materials.

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Section: Spr In Sensing In Particular For Conformational Changesmentioning

confidence: 99%

Section: Spr In Sensing In Particular For Conformational Changesmentioning

confidence: 99%

Real Time SPR Assessment of the Structural Changes of Adaptive Dynamic Constitutional Frameworks as a New Route for Sensing

et al. 2022

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“…It was clearly established that reduction of the isoalloxazine ring (riboflavin) occurs in two oneelectron steps and that there is a possibility of formation of a stable free radical termed a semiquinone. Using the Michaelis potentiometric formulation [81,89,90] the relationship between the maximum semiquinone formed and the potential, the difference in potential for the two steps (reduction of oxidized flavin to semiquinone (E 0' O/S )) and the reduction of the flavin to the reduced form (E 0' S/R ) the comproportionation constant can be calculated. This parameter, corresponding to the formation of the semiquinone from the oxidized and reduced forms can be deduced from the potentiometric titration curve as well as from SPEC in the visible region [74] and by EPR [32].…”

Section: Applications To Electroactive Proteins and Protein Derivativesmentioning

confidence: 99%

“…These studies provide some insight into flexibility of loading which is critical to efficient DET between the enzyme and the electrode [87]. Further studies have shown that shifts in the surface plasmon angle (Δθ r ) can be used to measure the distance between the α-and β-subunits [88,89].…”

Section: Applications To Electroactive Proteins and Protein Derivativesmentioning

confidence: 99%

Spectroelectrochemistry of Proteins

Wilson

2021

Electroanalysis

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This mini review addresses the evolution of spectroelectrochemistry from the laboratories of Professor Theodore Kuwana into the examination of proteins. Synchronizing electrochemical and spectral properties of proteins enables the identification of functions of redox centers, and their kinetics and thermodynamics. Changes in conformation and redox states are examined by a variety of spectroscopic techniques by directly observing protein reactions at electrodes with modified surfaces.

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“…It has been shown that the current density generated by the positively charged SAM is nearly 2 times higher than that by the methylation-modified positively charged SAM . In different reaction environments, CDH undergoes an open-to-close conformational change, which is governed by the solution pH. ,, For CDHs from different organisms, the optimal pH is different, and the IET is blocked at pH above 6 for most CDHs due to the open-state conformation . At neutral or alkaline pH, the deprotonation of amino acid side chains formed negatively charged patches at the domain interface, which leads to the electrostatic repulsion and results in an open-state conformation of Myriococcum thermophilum CDH ( Mt CDH) .…”

Section: Introductionmentioning

confidence: 99%

Molecular Insights of Cellobiose Dehydrogenase Adsorption on Self-Assembled Monolayers

Jian

2023

Langmuir

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Cellobiose dehydrogenase (CDH) is capable of direct electron transfer (DET) on electrodes and is a promising redox enzyme for bioelectrochemical applications. Its unique two-domain structure makes the function of CDH adsorbed on the surface of the electrode deeply affected by the external environment, such as ion species, strength, pH, and surface charge density. To date, however, the exact mechanism of how the external environment tailors the structure and dynamics of CDH adsorbed on the electrode surface still remains poorly understood. Here, multiscale simulations were performed to look for insight into the effect of Na+ and Ca2+ ions on the activation of CDH on oppositely charged self-assembled monolayer (NH2-SAM and COOH-SAM) surfaces with different surface charge densities (SCDs). Both Na+ and Ca2+ can promote CDH conformation switch from the open state to the closed state, while the promotion effect of Ca2+ is stronger than that of Na+ at the same conditions. However, the high ionic strength (IS) of Ca2+ renders the cytochrome (CYT) domain of CDH away from the NH2-SAM with low SCD. In contrast, whatever the IS, the NH2-SAM surface with high SCD can not only enhance the CYT–surface interaction but also achieve a closed-state conformation due to a similar role of Ca2+. Overall, this study gains molecular-level insights into the role of ion species and surface charge in modulating the structure and conformation of CDH on the SAM surface, thereby tailoring its activity.

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Determination of the Distance Between the Cytochrome and Dehydrogenase Domains of Immobilized Cellobiose Dehydrogenase by Using Surface Plasmon Resonance with a Center of Mass Based Model

Cited by 6 publications

References 35 publications

Real Time SPR Assessment of the Structural Changes of Adaptive Dynamic Constitutional Frameworks as a New Route for Sensing

Real Time SPR Assessment of the Structural Changes of Adaptive Dynamic Constitutional Frameworks as a New Route for Sensing

Spectroelectrochemistry of Proteins

Molecular Insights of Cellobiose Dehydrogenase Adsorption on Self-Assembled Monolayers

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