Determination of the effect of iatrogenic blood contamination on lactate dehydrogenase and creatine kinase activity in canine cerebrospinal fluid

Brune, J; Chen, Annie V.; Coffey, Todd

doi:10.1111/vcp.13184

Cited by 2 publications

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“…These differences in isoenzyme distribution may represent species‐specific variation or may be related to variances in detection methodology, storage parameters, collection techniques, and other factors. We are confident that the level of blood contamination accepted in the CSF of our reference individuals (<1000 RBC) is well below levels that were determined to affect total CK levels and isoenzyme activities 42 …”

Section: Discussionmentioning

confidence: 60%

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Creatine kinase and its isoenzymes in the serum and cerebrospinal fluid of healthy canines

Kopanke,

Chen,

Brune

et al. 2023

Veterinary Clinical Pathol

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BackgroundCreatine kinase (CK) exists as three isoenzymes (CK‐MM, CK‐MB, and CK‐BB) that are predominantly expressed in specific tissues and can be detected in both the serum and cerebrospinal fluid (CSF). CSF CK has been relatively unstudied in veterinary medicine, although studies in human medicine have demonstrated that changes in total CSF CK activity can indicate neurologic abnormalities.ObjectivesThe purpose of this study was to establish reference intervals for CK and its three major isoenzymes in the serum and CSF of clinically healthy dogs. By establishing a definitive reference interval for this enzyme in healthy canines, the diagnostic use and possible significance of CK in clinical disease can be studied.MethodsSerum and/or CSF were collected from healthy dogs. Total CK activity was measured spectrophotometrically, and isoenzyme distributions were determined using the QuickGel CK Vis Isoenzyme Kit and a densitometric scanner. Total CK and CK isoenzyme activities were determined within 8 h of collection.ResultsThe median serum total CK in healthy canines was 159.0 U/L (range: 53.0–539.0 U/L), while the median CSF total CK was 3.7 U/L (range: 2.0–84.0 U/L). CK‐BB and CK‐MM were approximately equal in the serum, while CK‐MM was the predominant isoenzyme in the CSF.ConclusionsKnowledge of the normal distribution and concentration of CK in canine serum and CSF will set the foundation for future studies of canine CK as a potentially clinically useful biomarker.

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Section: Discussionmentioning

confidence: 60%

“…We are confident that the level of blood contamination accepted in the CSF of our reference individuals (<1000 RBC) is well below levels that were determined to affect total CK levels and isoenzyme activities. 42 Clinically…”

Section: Discussionmentioning

confidence: 84%

Creatine kinase and its isoenzymes in the serum and cerebrospinal fluid of healthy canines

Kopanke,

Chen,

Brune

et al. 2023

Veterinary Clinical Pathol

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“…Активность ЛДГ определяли по Г.А. Кочетову [15,16], концентрацию белка -по методу Лоури в модификации M.H. Simonian и J.A.…”

Section: материалы и методыunclassified

Physical and Chemical Parameters in Assessment of Metabolic Profiles of Patients With B

Martusevich,

Soloveva,

Fedotova

et al. 2024

Innovacionnaâ medicina Kubani

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Objective: To study catalytic properties of lactate dehydrogenase (LDH) and some physical and chemical characteristics of blood in patients with thermal injuries.Materials and methods: We examined blood samples from 24 patients with contact burns involving 10%-40% of the body surface area and studied erythrocyte LDH activity in forward and reverse reactions, kinetic characteristics of this process, and protein concentration. We used teziocrystalloscopy to evaluate crystallogenic properties of blood serum of the examined patients with thermal injuries.Results: Thermal injuries were shown to cause a 2-fold decrease in LDH activity in the reverse reaction. Analysis of crystallogenic properties of blood serum of the patients with contact burns revealed that development of a complex response to a burn injury is accompanied by a moderate decrease in the structure index and crystallizability and a signifi decrease in the clearity of the marginal protein zone. In case of burn disease, we also observed a decrease in the initiative potential of the biological fl In addition, microscope slides of the dried blood serum revealed a tendency to randomization of the sample similar to that found in crystalloscopic facies.Conclusions: The local thermal effect leading to a complex response to a burn injury was found to cause significant transformation of physical and chemical characteristics of blood. Thermal injuries cause a decrease in LDH activity in the reverse reaction leading to a disturbance of the lactate-to-pyruvate ratio in the cell and significant shifts in the blood serum composition and properties, which are manifested in a significant change in blood serum crystallogenic and initiating properties.

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Determination of the effect of iatrogenic blood contamination on lactate dehydrogenase and creatine kinase activity in canine cerebrospinal fluid

Cited by 2 publications

References 39 publications

Creatine kinase and its isoenzymes in the serum and cerebrospinal fluid of healthy canines

Creatine kinase and its isoenzymes in the serum and cerebrospinal fluid of healthy canines

Physical and Chemical Parameters in Assessment of Metabolic Profiles of Patients With B

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