Determination of the effects of pre-harvest bagging treatment on kiwifruit appearance and quality via transcriptome and metabolome analyses

Jue, Dengwei; Sang, Xuelian; Li, Zhexin; Zhang, Wenlin; Liao, Qinhong; Tang, Jianmin

doi:10.1016/j.foodres.2023.113276

Cited by 3 publications

(3 citation statements)

References 63 publications

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“…The supernatant was collected as a source of chlorophyll. Absorbance was measured at 645 and 663 nm (AllSheng Company, Hangzhou, China) [32]. For flavonoid content detection, the potato tubers (1 g) were placed in a mortar and ground thoroughly in liquid nitrogen.…”

Section: Assay Of Anthocyanin Chlorophyll and Flavonoids In Purple Po...mentioning

confidence: 99%

The Impact of Different Nitrogen Levels on the Tuber Yield and Anthocyanin Synthesis of Purple Potatoes

Zhang,

Cai,

Guo

et al. 2024

Agriculture

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The biosynthesis of anthocyanins is influenced by external environmental conditions such as light, temperature, and nitrogen level, with nitrogen level being a key factor in anthocyanin synthesis and accumulation. Nitrogen level regulates the transcription factors involved in the anthocyanin synthesis pathway, with low nitrogen levels promoting anthocyanin accumulation, while high nitrogen levels have the opposite effect. Purple potatoes are a type of cultivated crop that is rich in anthocyanins and has unique economic value. Nitrogen fertilizer is crucial to improve the agronomic traits, yield, quality, and anthocyanin content of purple potatoes. In this study, the impact of four different nitrogen concentrations—0 kg/hm2 (N0), 90 kg/hm2 (N1), 225 kg/hm2 (N2) and 360 kg/hm2 (N3)—on the agronomic traits, yield, quality, and anthocyanin content of purple potatoes, ‘Huasong 66’, at different stages were investigated by using physiological index measurement and RNA-seq technology. It was found that the purple potato ‘Huasong 66’ was more sensitive to low nitrogen (N1). Under N1 level of nitrogen fertilization, ‘Huasong 66’ possessed the finest agronomic traits, yield, and quality, and the total anthocyanins in the tubers were significantly increased. Furthermore, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that nitrogen levels in purple potato tubers primarily affect genes related to nutrient transport and metabolism by regulating carbon and nitrogen metabolism, enzyme catalysis and binding, and signal transduction. In addition, nine candidate genes related to the anthocyanin synthesis pathway had been preliminarily screened. These results provide a basis to understand the impact of different nitrogen levels on the tuber yield and anthocyanin synthesis of purple potatoes.

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Section: Assay Of Anthocyanin Chlorophyll and Flavonoids In Purple Po...mentioning

confidence: 99%

The Impact of Different Nitrogen Levels on the Tuber Yield and Anthocyanin Synthesis of Purple Potatoes

Zhang,

Cai,

Guo

et al. 2024

Agriculture

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“…Pre‐harvest fruit metabolomic analysis can potentially assist in finding indicators for fruit quality and optimising harvesting tactics. The metabolites were subsequently analysed and identified with a variety of analytical methods, including mass spectrometry and nuclear magnetic resonance (Jue et al ., 2023). Pre‐harvest fruit metabolomic analysis can show metabolic changes in fruits as they develop and mature (Aroor et al ., 2023), as well as interactions between fruits and their environment.…”

Section: Fruit Metabolism and Metabolomicsmentioning

confidence: 99%

Recent progress in metabolomics research: insights into fruit metabolome, harvesting techniques and health‐promoting bioactives

Dadwal,

Aroor,

Joshi

et al. 2024

Int J of Food Sci Tech

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SummaryThe use of cutting‐edge omics technology to edible fruits has transformed the disciplines of fruit biology, pre‐ and post‐harvest investigations, metabolite biosynthesis and the identification of novel therapeutic fruit bioactives for health by leveraging varied omics data. Combining modern analytical chromatography tools (LC, GC) with mass spectrometry has significantly improved our ability to examine complex fruit tissues or extracted components, advancing our understanding of the fruit metabolome. Studies aiming at understanding the full metabolome and future quality characteristics have concentrated on quantifying the number of metabolites in edible fruit species and cultivars from diverse geographical locations. These studies have also helped to develop new databases for precise and comprehensive qualitative analysis of metabolites, allowing for the analysis of metabolite biosynthesis pathways to identify differences in metabolites among developed hybrids, metabolite origins and potential derivatives. Bioactive metabolite information is currently being utilised to manage illnesses, provide nutrition and creation of novel food products. Furthermore, this research has helped us better understand fruit quality and how metabolites interact with biological systems. In conclusion, this review emphasises the importance of metabolomics approaches in studying fruit metabolomes in the context of current research perspectives.

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“…In 'Hongbaoshixing' (a whole-fruit pigmented A. arguta cultivar), bagging treatment throughout fruit development substantially restricted the proper coloration of peel [18]. Although fruit bagging can enhance the fruit storability, flesh color, as well as flavonoid and anthocyanin contents of 'Hongyang', the underlying mechanism of these effects remains poorly understood [19][20][21]. Low temperatures stimulate anthocyanin accumulation in the plant kingdom [22,23], whereas high temperatures suppress anthocyanin accumulation [24,25].…”

Section: Introductionmentioning

confidence: 99%

AcMYB10 Involved in Anthocyanin Regulation of ‘Hongyang’ Kiwifruit Induced via Fruit Bagging and High-Postharvest-Temperature Treatments

Yu,

Xiong,

Dong

et al. 2024

Genes

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Light and temperature are key factors influencing the accumulation of anthocyanin in fruit crops. To assess the effects of fruit bagging during development and high post-ripening temperature on ‘Hongyang’ kiwifruit, we compared the pigmentation phenotypes and expression levels of anthocyanin-related genes between bagged and unbagged treatments, and between 25 °C and 37 °C postharvest storage temperatures. Both the bagging and 25 °C treatments showed better pigmentation phenotypes with higher anthocyanin concentrations. The results of the qRT-PCR analysis revealed that the gene expression levels of LDOX (leucoanthocyanidin dioxygenase), F3GT (UDP-flavonoid 3-O-glycosyltransferase ), AcMYB10, and AcbHLH42 were strongly correlated and upregulated by both the bagging treatment and 25 °C storage. The results of bimolecular fluorescence complementation and luciferase complementation imaging assays indicated an interaction between AcMYB10 and AcbHLH42 in plant cells, whereas the results of a yeast one-hybrid assay further demonstrated that AcMYB10 activated the promoters of AcLODX and AcF3GT. These results strongly suggest that enhanced anthocyanin synthesis is caused by the promoted expression of AcLODX and AcF3GT, regulated by the complex formed by AcMYB10–AcbHLH42.

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Determination of the effects of pre-harvest bagging treatment on kiwifruit appearance and quality via transcriptome and metabolome analyses

Cited by 3 publications

References 63 publications

The Impact of Different Nitrogen Levels on the Tuber Yield and Anthocyanin Synthesis of Purple Potatoes

The Impact of Different Nitrogen Levels on the Tuber Yield and Anthocyanin Synthesis of Purple Potatoes

Recent progress in metabolomics research: insights into fruit metabolome, harvesting techniques and health‐promoting bioactives

AcMYB10 Involved in Anthocyanin Regulation of ‘Hongyang’ Kiwifruit Induced via Fruit Bagging and High-Postharvest-Temperature Treatments

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