Determination of the efficiency of filtration of cultures from microalgae and bacteria using hollow fiber filters

Robla, J. I.; García-Hierrro, J.; Dittami, Simon M.; Marie, Dominique; Villa, Eva del Amor; Deragon, E.; Guillebault, Delphine; Mengs, Gerardo; Medlin, Linda K.

doi:10.1039/d0ew00927j

Cited by 2 publications

(3 citation statements)

References 15 publications

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“…In the rectangular cross-section, there were variations in the percentage of microalgae separation efficiency according to the fluid medium used. In the case of using distilled water as fluid medium, the separation efficiency varied from 60 to 100% (Schaap et al 2016 ; Robla et al 2021 ; Wang et al 2022 ; Lee and Yao 2018 ). The 100% separation efficiency obtained in the study by (Robla et al 2021 ) was because of using a nonporous membrane as sandwiched between the microfluidics channels during cell separation.…”

Section: Resultsmentioning

confidence: 99%

“…In the case of using distilled water as fluid medium, the separation efficiency varied from 60 to 100% (Schaap et al 2016 ; Robla et al 2021 ; Wang et al 2022 ; Lee and Yao 2018 ). The 100% separation efficiency obtained in the study by (Robla et al 2021 ) was because of using a nonporous membrane as sandwiched between the microfluidics channels during cell separation. The use of polyethylene oxide solution as a fluid medium, resulted in a higher separation efficiency of 97.5 to 100% (Liu et al 2022 ; Yuan et al 2019 ).…”

Section: Resultsmentioning

confidence: 99%

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Separation of microalgae from bacterial contaminants using spiral microchannel in the presence of a chemoattractant

Ngum,

Matsushita,

El-Mashtoly

et al. 2024

Bioresour. Bioprocess.

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Cell separation using microfluidics has become an effective method to isolate biological contaminants from bodily fluids and cell cultures, such as isolating bacteria contaminants from microalgae cultures and isolating bacteria contaminants from white blood cells. In this study, bacterial cells were used as a model contaminant in microalgae culture in a passive microfluidics device, which relies on hydrodynamic forces to demonstrate the separation of microalgae from bacteria contaminants in U and W-shaped cross-section spiral microchannel fabricated by defocusing CO2 laser ablation. At a flow rate of 0.7 ml/min in the presence of glycine as bacteria chemoattractant, the spiral microfluidics devices with U and W-shaped cross-sections were able to isolate microalgae (Desmodesmus sp.) from bacteria (E. coli) with a high separation efficiency of 92% and 96% respectively. At the same flow rate, in the absence of glycine, the separation efficiency of microalgae for U- and W-shaped cross-sections was 91% and 96%, respectively. It was found that the spiral microchannel device with a W-shaped cross-section with a barrier in the center of the channel showed significantly higher separation efficiency. Spiral microchannel chips with U- or W-shaped cross-sections were easy to fabricate and exhibited high throughput. With these advantages, these devices could be widely applicable to other cell separation applications, such as separating circulating tumor cells from blood. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Separation of microalgae from bacterial contaminants using spiral microchannel in the presence of a chemoattractant

Ngum,

Matsushita,

El-Mashtoly

et al. 2024

Bioresour. Bioprocess.

View full text Add to dashboard Cite

show abstract

“…To perform a molecular-level analysis at time-point zero, it would have been necessary to filter the samples to gather the existing microbiome. However, this filtration process could have caused the loss of picoplankton [51] or dormant cysts, potentially resulting in the omission of certain species [52]. We recognize that conducting molecular analysis on the three samples before enrichment on day zero would have further enhanced our understanding of the algal population dynamics from the beginning.…”

Section: Plos Onementioning

confidence: 99%

Comparative study of multiple approaches for identifying cultivable microalgae population diversity from freshwater samples

Badr

Fouad

2023

PLoS ONE

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The vast diversity of microalgae imposes the challenge of identifying them through the most common and economical identification method, morphological identification, or through using the more recent molecular-level identification tools. Here we report an approach combining enrichment and metagenomic molecular techniques to enhance microalgae identification and identify microalgae diversity from environmental water samples. From this perspective, we aimed to identify the most suitable culturing media and molecular approach (using different primer sets and reference databases) for detecting microalgae diversity. Using this approach, we have analyzed three water samples collected from the River Nile on several enrichment media. A total of 37 microalgae were identified morphologically to the genus level. While sequencing the three-primer sets (16S rRNA V1-V3 and V4-V5 and 18S rRNA V4 region) and aligning them to three reference databases (GG, SILVA, and PR2), a total of 87 microalgae were identified to the genus level. The highest eukaryotic microalgae diversity was identified using the 18S rRNA V4 region and alignment to the SILVA database (43 genera). The two 16S rRNA regions sequenced added to the eukaryotic microalgae identification, 26 eukaryotic microalgae. Cyanobacteria were identified through the two sequenced 16S rRNA regions. Alignment to the SILVA database served to identify 14 cyanobacteria to the genera level, followed by Greengenes, 11 cyanobacteria genera. Our multiple-media, primer, and reference database approach revealed a high microalgae diversity that would have been overlooked if a single approach had been used over the other.

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Determination of the efficiency of filtration of cultures from microalgae and bacteria using hollow fiber filters

Abstract: The most important question in sampling is “Is the sample representative of the target population?” This question is necessary to understand how valid the sample taken is to the original...

Cited by 2 publications

References 15 publications

Separation of microalgae from bacterial contaminants using spiral microchannel in the presence of a chemoattractant

Separation of microalgae from bacterial contaminants using spiral microchannel in the presence of a chemoattractant

Comparative study of multiple approaches for identifying cultivable microalgae population diversity from freshwater samples

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