Determination of the Enantiomeric Composition of a Novel Topically Active Carbonic Anhydrase Inhibitor by HPLC

Novak, Thomas J.; Berwick, Lorrie

doi:10.1080/10826079808005899

Cited by 7 publications

(3 citation statements)

References 24 publications

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“…These include LC-MS [18], TLC-densitometry, first-derivative UVspectrophotometry and ratio derivative spectrophotometry [19], spectroscopic methods [20], HPLC method with diodearray and UV detection [21], and voltammetry [22]. There are also a few reports on the indirect chiral separation of dorzolamide in the literature [23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Chiral separation and quantitation of dorzolamide hydrochloride enantiomers by high‐performance liquid chromatography

Shamsipur

Heydari

Jamasbi³

et al. 2010

J of Separation Science

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A direct HPLC method for chiral separation of dorzolamide hydrochloride (4S,6S) and its enantiomer (4R,6R) was developed. Dorzolamide (4S,6S) and its antipode were separated on a chiral-alpha(1)-acid glycoprotein column (150 x 4.0 mm, 5 microm). The influences of pH, temperature, flow rate, buffer concentration, and organic modifiers of the mobile phase on the retention and enantioselectivity were evaluated. The mobile phase consisted of an ammonium acetate buffer of pH 7.0. The method was validated for linearity, repeatability, accuracy, LOD, and LOQ. Calibration curves were constructed in the range of 0.5-10 microg/mL for dorzolamide (4S,6S) and 0.2-5 microg/mL for its enantiomer (4R,6R). Repeatability (n=6) showed less than 2% RSD. LOD and LOQ of the two enantiomers were found to be 0.2 and 0.5 for dorzolamide (4S,6S), 0.05 and 0.2 for its enantiomer (4R,6R), respectively. The proposed method was applied to the determination of dorzolamide enantiomer (4R,6R) in a raw material and two different eye drop samples.

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Section: Introductionmentioning

confidence: 99%

Chiral separation and quantitation of dorzolamide hydrochloride enantiomers by high‐performance liquid chromatography

Shamsipur

Heydari

Jamasbi³

et al. 2010

J of Separation Science

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“…The literature review reveals that several methods have been reported for the estimation of DORZO in biological fluids [2][3][4][5], and there are some methods reported by HPLC [6][7][8][9], spectroscopy [10,11], and capillary electrophoresis [12], and there are some methods reported for estimation of DORZO in pharmaceutical formulation [13][14][15]. According to current good manufacturing practices, all drugs must be tested with a stability-indicating assay method before release.…”

Section: Introductionmentioning

confidence: 99%

Stability-Indicating HPTLC-Densitometric Method for Estimation of Dorzolamide Hydrochloride in Eye Drops

Havele

Dhaneshwar

2012

ISRN Analytical Chemistry

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The present study describes the degradation of dorzolamide HCl under different International Conference on Harmonization prescribed stress conditions (hydrolysis, oxidation, thermolysis, and photolysis) and application of a specific and selective stabilityindicating chromatography assay. HPTLC was done on silica Gel 60 F 254 TLC plate using chloroform: methanol in the ratio of 9.0 : 1.0 (v/v) as mobile phase and detection at 253 nm.

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“…The literature survey reveals that both dorzolamide hydrochloride and timolol maleate have been analyzed by various techniques. Several methods have been reported for the determination of DOR and TIM in biological fluids [3][4][5][6] by HPLC [7][8][9][10], spectrophotometry [11][12][13], capillary electrophoretic (CE) [14], and there are some methods reported for estimation of DOR in pharmaceutical formulations [8,[15][16]. Electrochemical detection is a very elegant method in the pharmaceutical analysis due to its high sensitivity, rapid response.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical and Chemometric Determination of Dorzolamide and Timolol in Eye Drops Using Modified Multiwall Carbon Nanotube Electrode

Hendawy

Elwy

Fekry

2017

Int J Pharm Pharm Sci

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Objective: This work is focused on the construction of simple and sensitive electrochemical sensor for quantitative determination of dorzolamide (DOR) and timolol maleate (TIM). This method is based on the incorporation of multiwall carbon nanotubes (MWCNT) into the carbon paste electrode which improve the characteristics of the electrode. Methods:The electrochemical response of modified electrode was based on voltammetric oxidation, using cyclic voltammetry (CV) and impedance spectroscopy (EIS). The structural morphology of the surface modified electrode was characterized by scanning electron microscope (SEM). Quantitative analysis for each of the two compounds in a mixture has been examined by using of chemometric tools for resolving overlapping signals. The prediction performance of the chemometric method was analyzed by principal component regression (PCR) and partial least square (PLS).Results: Fractional factorial design was constructed from set of synthetic mixtures of two drugs in concentration ranges of 0.05 to 1.6µg/ml for DOR and 1.5-20 µg/ml for TIM. Under optimum experimental conditions, DOR and TIM gave rectilinear response over the concentration range of 0.072-1.88 µg/ml and 1.16-20.84 µg/ml, respectively. The limit of detection (LOD) was found to be 0.098 and 1.025 µg/ml, for DOR and TIM, respectively. It found that the % of relative prediction error (RPE) was acceptable and satisfactory. Conclusion:In these work, for the first time, a new voltammetric simultaneous method developed for a rapid and efficient determination of DOR and TIM from eye dropper sample at nano modified electrode with satisfactory results. These results indicate that MWCNT holds great promise in practical application.

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Determination of the Enantiomeric Composition of a Novel Topically Active Carbonic Anhydrase Inhibitor by HPLC

Cited by 7 publications

References 24 publications

Chiral separation and quantitation of dorzolamide hydrochloride enantiomers by high‐performance liquid chromatography

Chiral separation and quantitation of dorzolamide hydrochloride enantiomers by high‐performance liquid chromatography

Stability-Indicating HPTLC-Densitometric Method for Estimation of Dorzolamide Hydrochloride in Eye Drops

Electrochemical and Chemometric Determination of Dorzolamide and Timolol in Eye Drops Using Modified Multiwall Carbon Nanotube Electrode

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