Determination of the Fatty Acid Composition in Tree Peony Seeds Using Near‐Infrared Spectroscopy

Abstract

Tree peonies (Paeonia Sect Moutan DC) are an emerging oil crop because of their high oil and α‐linolenic acid (ALA) content. This research was to investigate the potential use of near infrared reflectance spectroscopy (NIRS) for estimating the major fatty acids contents, such as palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acid (C18:3) in tree peonies. A total of 115 small seed samples and 447 single seeds were selected to calibrate the predictive models. NIRS absorbance spectra were collected using a Fourier transform near infrared (FT‐NIR) spectrometer for the small seed samples, and acousto‐optic tunable filter‐near infrared (AOTF‐NIR) for the single seed samples. Statistical analysis was performed with partial least squares (PLS). For the husked samples, C18:3, C18:1 and C18:2 showed the highest correlation coefficient of validation (Rv; = 0.9756, 0.9467 and 0.8485, respectively) and the ratio of performance to deviation (RPD; = 3.58, 1.91 and 2.17, respectively); however, C16:0 did not reach expectations (Rv = 0.7783, RPD = 1.32). For intact samples, C18:3 showed the best prediction (Rv = 0.9096, RPD = 3.14), followed by C18:2 (Rv = 0.8479, RPD = 1.96). The results for C18:1 were poor (Rv = 0.7237, RPD = 1.70). For single seeds, only the results for C18:3 (R = 0.9150, RPD = 1.73) were good in the husked seed samples. It was concluded that NIRS can be used to rapidly assess the content of the major fatty acids in small samples.

Genetic analyses reveal independent domestication origins of the emerging oil crop Paeonia ostii, a tree peony with a long-term cultivation history

“…Therefore, P. ostii is an important economic plant with simultaneous medicinal and oil applications. The rediscovery of the value of this oil crop and its rapid deployment in recent years represent unprecedented opportunities for farmers and have attracted considerable interest among plant breeders 10, 16, 17 .…”

Development of non‐destructive models to predict oil content and fatty acid composition of Gomenzer (Ethiopian mustard) using near‐infrared reflectance spectroscopy

“…From the detected 15 fatty acids, only seven fatty acids were proposed for NIRS model development. As the NIRS method cannot be used to predict components at lower percentage values, 28 the study focused on the major fatty acid components present in Ethiopian mustard. The statistical values, including mean, standard deviation (SD), minimum, and maximum, of the individual selected fatty acids and oils of Ethiopian mustard used in the calibration and validation sets are shown in Table 1.…”

“…In Ethiopian mustard oil samples, more than 15 fatty acids were detected and identified by GC-MS. From the detected 15 fatty acids, only seven fatty acids were proposed for NIRS model development. As the NIRS method cannot be used to predict components at lower percentage values, 28…”

Dissection of Allelic Variation Underlying Floral and Fruit Traits in Flare Tree Peony (Paeonia rockii) Using Association Mapping

“…Studies have shown that the MYB5 gene of PS12 is involved in seed coat development to control seed size and affect yield (Li et al, 2008;Su et al, 2011;Dong et al, 2017). The oil content in tree peony seeds can reach 27-33%, which can directly affect seed weight (Cui et al, 2016). The WRIL1 gene corresponding to P280 may be an important gene regulating oil synthesis in tree peony.…”