Determination of the Fatty Acid Profile of Fish by a One‐Step Extraction/Methylation Method

Abstract

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Nutrient Composition of Bison Fed Concentrate Diets

“…Fatty acids were determined by the procedure of Ulberth and Henniger (1995) using the following conditions. Fatty acid methyl esters (FAME) were separated by means of a 0.32 mm ϫ 30 m fused silica capillary column (10% SP-2340), Supelco, Inc., in a Shimadzu GC-9A chromatograph with autosampler.…”

Development in lipid analysis: Some new extraction techniques and in situ transesterification

“…Different solvents have been used in performing in situ acid-based methods: hexane (100-102), tetrahydrofuran (79), benzene (74,75,79,103,104), toluene (75,77,79,105), and chloroform (75). Pentane, hexane, or isooctane were not beneficial when used to replace benzene (74), and benzene and toluene extracted FAME better than chloroform (75).…”

Comprehensive lipidome profiling of Sauvignon blanc grape juice

“…Although a number of methods have been described for fatty acid profiling (Abdulkadir & Tsuchiya, 2008;Akoto, Vreuls, Irth, Pel, & Stellaard, 2008;Armstrong, Metherel, & Stark, 2008;Cantellops, Reid, Eitenmiller, & Long, 1999;Connerth, Grillitsch, Kofeler, & Daum, 2009;Glaser, Demmelmair, & Koletzko, 2010;Mazalli & Bragagnolo, 2007;Paterson & Amado, 1997;Quehenberger et al, 2011;RodriguezPalmero, Lopez-Sabater, Castellote-Bargallo, Dela Torre-Boronat, & Rivero-Urgell, 1997;Ulberth & Henninger, 1995;Wiesman & Chapagain, 2009), they often involve laborious procedures with several extraction steps and a combination of different solvents, anhydrous conditions and freezing cycles, which make sample preparation very time consuming. Moreover, the high sugar content in grape juice poses a significant problem to profile and quantify lipids at their trace levels due to the matrix effect.…”