Determination of the hydroxyl radical by its adduct formation with phenol and liquid chromatography/electrochemical detection

Kılınc, Emrah

doi:10.1016/j.talanta.2004.08.019

Cited by 34 publications

(22 citation statements)

References 14 publications

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“…The probe compound should be characterized by few and very stable reaction products and by a well-known and established reaction constant with reactive radicals. Dimethyl sulfoxide [15], salicylic acid [16], phenols or pyrocathecols [17] are among the most commonly used compounds. Recently, Lindsey and Tarr [18] selected benzoic acid and n-propanol as chemical probes to quantify the hydroxyl radicals production in Fenton's systems, allowing to measure both hydroxyl radical formation rate and concentration.…”

Section: Introductionmentioning

confidence: 99%

Influence of the operating conditions on highly oxidative radicals generation in Fenton's systems

Ciotti

Baciocchi

Tuhkanen

2009

Journal of Hazardous Materials

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a b s t r a c tIn this work, an indirect method for estimating the total amount and concentration of oxidative radicals in aqueous and slurry-phase Fenton's systems was developed. This method, based on the use of benzoic acid as probe compound, was applied for evaluating the effect of the operating conditions on the radicals amount produced, their production efficiency (i.e. moles of radicals generated per mole H 2 O 2 ) and their concentration. A Rotatable Central Composite design (RCC) was used to select the operating conditions in order to get a statistically meaningful data set. Hydrogen peroxide and ferrous ion concentrations ranged between 0.2-1 mM and 0.2-0.5 mM, respectively; humic acid concentration between 0 and 15 mg/L, whereas the soil/water weight ratio in slurry-phase systems between 1:10 and 9:10. The probe compound concentration was 9 or 0.1 mM in experiments aimed to evaluate the total amount or concentration of oxidative radicals, respectively. The obtained results indicated that the amount of radicals generated in both aqueous and soil slurry Fenton's system increased with higher H 2 O 2 concentration and, more specifically, that their production efficiency increased with increasing Fe(II):H 2 O 2 molar ratio. Addition of dissolved organic compounds as humic acid did not notably affect the oxidative radicals amount and concentration. On the contrary, a one order of magnitude reduction in both radicals amount generated and concentration was observed when soil was added to the reaction environment.

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Section: Introductionmentioning

confidence: 99%

Influence of the operating conditions on highly oxidative radicals generation in Fenton's systems

Ciotti

Baciocchi

Tuhkanen

2009

Journal of Hazardous Materials

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“…Copper is a component of several metalloenzymes. Zinc There are several methods for free radical determination, including fluorescence 7) and liquid chromatography 8) . However, we selected ESR because it is specific and reliable.…”

Section: Effect Of Interaction Between Antioxidants and Metal Ions Onmentioning

confidence: 99%

Generation of Reactive Oxygen Species by Interaction between Antioxidants Used as Food Additive and Metal Ions

Iwasaki

Oda²,

Tsukuda³

et al. 2014

Journal of the Food Hygienics Society of Japan

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Food additives, such as preservatives, sweeteners, coloring agents, and flavoring agents, are widely used in food manufacturing. However, their combined effects on the human body are not known. The purpose of this study was to examine whether combinations of antioxidants and metal ions generate reactive oxygen species (ROS) under in vitro conditions using electron spin resonance (ESR). Among the metal ions examined, only iron and copper generated ROS in the presence of antioxidants. Moreover, certain phenolic antioxidants having pro-oxidant activity induced DNA oxidation and degradation via the generation of high levels of ROS in the presence of copper ion, resulting in complete degradation of DNA in vitro.

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“…Commonly used methods for detecting · OH include electron spin resonance (ESR) [7][8][9] ; chemiluminescence 10,11) ; and high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection, 12) electrochemical detection (ED), [13][14][15] and fluorometric detection. 16,17) The ESR method has been widely accepted to measure · OH scavenging capacity, but it requires expensive instrumentation and cannot be readily used to obtain quantitative estimates of · OH adducts because the · OH spin is unstable and may react with other products.…”

Section: Notesmentioning

confidence: 99%

Selective Detection of Hydroxyl Radical Scavenging Capacity Based on Electrogenerated Chemiluminescence Detection Using Tris(2,2'-bipyridine)ruthenium(III) by Flow Injection Analysis

Nobushi

Uchikura

2010

CHEMICAL & PHARMACEUTICAL BULLETIN

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We describe a new method for detecting hydroxyl radical scavenging capacity based on tris(2,2-bipyridine)ruthenium(III) [Ru(bpy) 3 3؉] chemiluminescence and flow injection analysis. Hydroxyl radicals were generated by the Fenton reaction. The scavenging capacity of the six antioxidants tested decreased in the following order: edaravoneϾL-tryptophanϾgallic acidϾTroloxϾN-acetyl-L-cysteineϾascorbic acid. The proposed method allowed a sample throughput of about 80 samples/h. The six antioxidants were found to inhibit chemiluminescence intensity of Ru(bpy) 3 2؉. The proposed method is a rapid, selective, and accurate procedure for the study of hydroxyl radical scavenging capacity by Ru(bpy) 3 3؉ ؉ chemiluminescence.

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Determination of the hydroxyl radical by its adduct formation with phenol and liquid chromatography/electrochemical detection

Cited by 34 publications

References 14 publications

Influence of the operating conditions on highly oxidative radicals generation in Fenton's systems

Influence of the operating conditions on highly oxidative radicals generation in Fenton's systems

Generation of Reactive Oxygen Species by Interaction between Antioxidants Used as Food Additive and Metal Ions

Selective Detection of Hydroxyl Radical Scavenging Capacity Based on Electrogenerated Chemiluminescence Detection Using Tris(2,2'-bipyridine)ruthenium(III) by Flow Injection Analysis

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