Determination of the main tropane alkaloids from transformed Hyoscyamus muticus plants by capillary zone electrophoresis

Eeva, Manu; Salo, Jukka-Pekka; Oksman-Caldentey, Kirsi-Marja

doi:10.1016/s0731-7085(97)00121-0

Cited by 48 publications

(14 citation statements)

References 10 publications

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“…In the pH range 2.5-5.0, atropine and anisodamine were baseline separated, and scopolamine and atropine were only partial separated. Contrary to a previous report (Eeva et al, 1998), we could not separate the three compounds at pH 7.8. At pH 2.5 and pH 5.0 the best resolution can be obtained for the separation of scopolamine and atropine.…”

Section: Effect Of Buffer Phcontrasting

confidence: 99%

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Determination of scopolamine, atropine and anisodamine in Flos daturae by capillary electrophoresis

Ye¹,

Zhu²,

Gu³

et al. 2001

Biomedical Chromatography

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A capillary electrophoresis method was developed for the separation and determination of tropane alkaloids in Flos daturae plants. Separation was performed on a fused silica capillary(42.1 cm x 50 microm i.d.) at an applied voltage of 20 kV. Scopolamine, atropine and anisodamine were well separated in the buffer of 50 mmol/L phosphate buffer (pH 5.0) containing 20% (v/v) tetrahydrofuran (THF). Beer's law was obeyed in the range of concentration of 2.4-21.8 microg/mL for scopolamine, 4.0-36.0 microg/mL for atropine and 2.6-23.7 microg/mL for anisodamine, respectively, and the correlation coefficients were over 0.999 (n = 6). The developed method was applied for the analysis of herb samples.

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Section: Effect Of Buffer Phcontrasting

confidence: 99%

“…The tropane alkaloids in hairy roots have been assayed by micellar electrokinetic chromatography (Mateus et al, 1998). The main tropane alkaloids from transformed Hyoscyamus muticus plants have been determinated by capillary zone electrophoresis employing a 40 mmol/L phosphate buffer at pH 7.8 (Eeva et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Determination of scopolamine, atropine and anisodamine in Flos daturae by capillary electrophoresis

Ye¹,

Zhu²,

Gu³

et al. 2001

Biomedical Chromatography

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“…1), this indicates that scopolamine is more familiar with the material of the gaseous chromatographic column, and more volatile with the used inert gas. These results accord with that of Eava et al (1998)) on H. muticus. To estimate the probable somaclonal variation derived from in vitro and callus culture, we subjected the wild plants, the in vitro plants and the callus on line A and B media to ISSR analysis.…”

Section: Resultssupporting

confidence: 91%

Production of tropan alkaloids in the in vitro and callus cultures of Hyoscyamus aureus and their genetic stability assessment using ISSR markers

Besher

Al-Ammouri

Murshed

2014

Physiol Mol Biol Plants

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Green wild plants (dirctly before flowering) and seeds of Hyoscyamus aureus were collected from natural habitat at Al Qalamon region in Syria. Seeds were surface sterilized and cultured in vitro, after 21 days from germination stem-derived callus was induced on two different nutrient media. Tropane alkaloids were extracted from wild plants and 30 days old in vitro plants and callus, and then analyzed using GC-MS. Genetic variation was also studied between the wild and in vitro plants and the callus culture lines using twenty ISSR markers. The results showed that there were significant variations in tropane alkaloids contents between the wild plants, the in vitro plants and the callus culture lines. The highest content of hyoscyamine was in callus on line A medium, but the highest content of scopolamine was in the wild plants. However, the lowest content of tropane alkaloids was in callus on line B medium. Also the ISSR analyses showed that there was genetic variation between the wild and in vitro plants and the callus culture lines.

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“…1) have considerable pharmacological implications. Atropine is normally used as a parasympatolytic, anticholinergic, spasmolytic and antiemetic drug [4,5]. Choline is a precursor of essential compounds such as the membrane lipids phosphatidylcholine and sphingomyelin, the neurotransmitter acetylcholine and the signaling lipids plateletactivating factor and sphingosylphosphorylcholine [6,7].…”

Section: Introductionmentioning

confidence: 99%

Analysis of choline and atropine in hairy root cultures of Cannabis sativa L. by capillary electrophoresis‐electrospray mass spectrometry

et al. 2006

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We describe a capillary zone electrophoresis method coupled to electrospray ionization (ion trap) mass spectrometry (CZE-ESI-MS) for the identification and determination of choline and atropine compounds in hairy root extracts from Cannabis sativa L. Fused-silica capillary and an alkaline solution of 20 mM ammonium acetate at pH 8.5 are used being the most suitable for the analysis of choline and atropine in less than 10 min. Under the optimized conditions, including CE and ESI-MS parameters, the method resolved both compounds with very high sensitivity. The system exhibited good linear response in the range of 25-500 mg/L and 500-1000 microg/L for choline and atropine, respectively. The detection limit of choline was 18 mg/L and 320 microg/L for atropine. Finally, the developed method was applied to the analysis of these compounds in transgenic root cultures of Cannabis sativa L.

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Determination of the main tropane alkaloids from transformed Hyoscyamus muticus plants by capillary zone electrophoresis

Cited by 48 publications

References 10 publications

Determination of scopolamine, atropine and anisodamine in Flos daturae by capillary electrophoresis

Determination of scopolamine, atropine and anisodamine in Flos daturae by capillary electrophoresis

Production of tropan alkaloids in the in vitro and callus cultures of Hyoscyamus aureus and their genetic stability assessment using ISSR markers

Analysis of choline and atropine in hairy root cultures of Cannabis sativa L. by capillary electrophoresis‐electrospray mass spectrometry

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