Determination of the myofibrillar and connective tissue proteins in the bovine diaphragm

Comparison of the total protein, nitrogen, and amino acid composition of selected additives and ingredients used in composite meat products

“…A comparison of the essential amino acid profile of potato protein given in Table II (3115 mg/g of N) with the essential amino acid profiles of the whole egg indicated that this protein supplement was only slightly lower than either hen's whole egg (3215 mg/g of N) or cow's milk (3200 mg/g of N) proteins (FAO/WHO, 1965). Moreover, when comparisons of the essential amino acid profile of alfalfa (2752 mg/g of N) were made with other proteins (Table II), it was found that alfalfa leaf protein was higher 0 Determined by the methods described previously (Zarkadas et al, 1988).…”

“…The unusual amino acid calibration standards employed for peak identification and standardization of the instrument were prepared essentially as described previously (Zarkadas, 1975(Zarkadas, ,1979, using Tyr(N02) as the internal standard (Zarkadas et al, 1987b). Recoveries of these unique basic amino acids were calculated on the basis of protein content of individual hydrolysates determined according to Horstmann (1979) as described previously (Zarkadas et al, 1988).…”

Comparison of the Protein Quality of Five New Northern Adapted Natto Soybean Cultivars by Amino Acid Analysis

“…Duplicate samples (0.05 g) were hydrolyzed in Pyrex (No. 9860) test tubes (18 × 150 mm) under vacuum (below 10 mmHg) with triple glassdistilled constant-boiling HCl (6.0 M) containing 0.2% (v/v) phenol at 110 ( 0.5 °C for periods of 24, 48, 72, and 96 h with the usual precautions described by Zarkadas et al (1988b). Analyses of individual acid hydrolysates were performed on the clear filtrate in duplicate according to methods described previously (Zarkadas et al, 1986(Zarkadas et al, , 1988b.…”

Comparison of the amino acid composition and protein contents of two northern adapted asparagus cultivars

“…The clear filtrate and washings were combined, evaporated to dryness in a Rotary Evapomix (Buchler Instruments, Fort Lee, NJ) at 45 °C, and brought to volume with 0.2 M sodium citrate buffer, pH 2.2. Norleucine and 3-nitrotyrosine, selected as the internal standards, were included in this step or prior to hydrolysis (Zarkadas et al, 1987(Zarkadas et al, ,1988c.…”