Determination of the optimal concentration of Minitube Equex paste for boar semen cryopreservation based on sperm motility characteristics

Suwimonteerabutr, Junpen; Nuntapaitoon, Morakot; Tummaruk, Padet

doi:10.46419/vs.52.3.8

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…Therefore, cryopreservation of boar semen is the only method to preserve superior genetic boar semen (Jovičić et al, 2020). Moreover, cryopreserved boar semen can be exported across countries for improving swine genetics (Suwimonteerabutr et al, 2021). Another advantage of using frozen-thawed boar semen is the limitation of the risk of disease transmission among herds.…”

Section: Introductionmentioning

confidence: 99%

“…The frozen-thawed boar semen process causes a significant reduction in sperm motility, viability, acrosome integrity, DNA integrity and fertilising ability (Yeste, 2016;Rungruangsak et al, 2021;Suwimonteerabutr et al, 2021). Studies have been conducted to improve the quality of frozen-thawed boar semen including the cryopreservation process, semen extender, thawing media and searching for a freezability marker (Buranaamnuay et al, 2009;Chanapiwat et al, 2010;Yeste, 2015;Jovičić et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Razlika između proteina sjemene plazme i proteina sperme za dobru i lošu sposobnost smrzavanja ejakulata nerasta

Rungruangsak¹,

Suwimonteerabutr

Buranaamnuay

et al. 2021

Vet. stn. (Online)

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The present study was performed to compare the expression of sperm proteins, i.e. triosephosphate isomerase (TPI) and acrosin binding protein (ACRBP) and seminal plasma proteins, i.e. glutathione peroxidase 5 (GPX5) and fibronectin 1 (FN1), in boar semen with good, moderate and poor freezability. The study was conducted by determining the protein contents in 32 sperm samples and 38 seminal plasma samples of semen. The ejaculated semen was divided into two portions: the first portion was centrifuged to separate the pellet of sperm from the seminal plasma and the second portion was cryopreserved. After thawing, the ejaculates were classified into three groups according to their post-thawed sperm motility: good (60.2 ± 1.7%), moderate (29.3 ± 2.0%) and poor (16.6 ± 2.2%) freezabilities. The expressions of GPX5 and FN1 in seminal plasma and TPI and ACRBP in sperm were determined using Western blot analysis. It was found that, for sperm proteins, the level of TPI was negatively correlated with the post-thawed total sperm motility (r = -0.38, P = 0.029). For seminal plasma proteins, the level of FN1 in the seminal plasma was positively correlated with the post-thawed total sperm motility (r = 0.37, P = 0.021) and progressive motility (r = 0.39, P = 0.016). The expression of GPX5 was not correlated with any of the frozen–thawed sperm qualities (P > 0.05). In conclusions, boar semen containing a high level of FN1 in seminal plasma has better freezability. Frozen–thawed sperm motility was positively correlated with the level of FN1 in boar seminal plasma and negatively correlated with TPI in boar spermatozoa.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Razlika između proteina sjemene plazme i proteina sperme za dobru i lošu sposobnost smrzavanja ejakulata nerasta

Rungruangsak¹,

Suwimonteerabutr

Buranaamnuay

et al. 2021

Vet. stn. (Online)

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Cryoprotectant effects of natural honey on spermatozoa quality of pre-freezing and frozen-thawed boar semen

Balogun

Nicholls

Sokunbi

et al. 2022

Journal of Animal Science

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Natural honey has been successfully used in the preservation of mammalian gametes because of its beneficial properties. The objectives of this study were to determine the inclusion level of honey in extender for improving boar semen quality before freezing and to investigate the effects of honey inclusion in extender and freezing media on post-thaw quality of frozen-thawed boar semen samples. Ejaculates from 6 terminally crossbred boars were collected using the gloved-hand technique for two experiments. Experiment 1 was a randomized block design, evaluating 4 inclusion levels of honey in boar semen extender [Control (0H) – Androhep Plus or Androhep Plus with 0.25, 0.50 and 0.75% honey (0.25H, 0.50H and 0.75H respectively)]. Ejaculates were pooled, aliquoted according to treatments, and cooled for 24 hours at 17ºC. The results of this experiment were used to determine inclusion levels in Exp. 2. Experiment 2 was a 2 x 3 factorial design, evaluating the inclusion of honey in boar semen extender and freezing media. Semen samples from individual boars were cooled in extender with or without honey (C0: Androhep Plus; C1: Androhep Plus + 0.25% honey). After 24 hours, semen samples were evaluated, diluted in lactose-egg yolk (LEY) media, and one of three freezing media types; F0: 93% LEY + 6% glycerol + 1% Equex-STM Paste (ESP); F1: 93% LEY + (3% glycerol and 3% honey) + 1% ESP; and F2: 93% LEY + 6% glycerol + (0.5% ESP and 0.5% honey). Samples were frozen in 0.5mL straws using a controlled-rate freezer and stored in liquid nitrogen. In Exp. 1, 0.25H and 0.50H improved motility (P=0.033) and progressive motility (P=0.001) of cooled boar semen. Nevertheless, 0.25H was selected for Exp. 2. In Exp. 2, post-thaw motility and progressive motility were highest (P<0.05) in C0F2 but not different from C1F2. Morphologically normal cells and acrosomes were higher with all inclusion levels of honey (P<0.05). In conclusion, 0.25 and 0.50% inclusion of honey in Androhep Plus improves motility and progressive motility of cooled boar semen samples after 24 hours. Supplementing Androhep Plus with 0.25% honey maintains higher normal sperm cells and acrosomes of cryopreserved boar semen. Replacing 50% Equex-STM paste with honey in freezing media improves post-thaw sperm motility, progressive motility, percentage normal sperm and acrosome of cryopreserved boar semen.

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Supplementation of glutamine in a short-term boar semen extender during 17°C holding time enhances post-thaw sperm quality for cryopreservation

Nuntapaitoon,

Tummaruk,

Suwimonteerabutr

2024

Porc Health Manag

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Background Glutamine is a nonessential amino acid and the most abundant amino acid found in the seminal plasma and sperm-rich fraction of boar semen. Glutamine plays an important role in enhancing glutathione (GSH) synthesis. It acts as an effective antioxidant in semen and provides intracellular defense to sperm against oxidative stress. This study aimed to improve the quality of frozen-thawed boar semen by using glutamine supplementation in a short-term semen extender during the holding time at 17 °C before cryopreservation. Results The results indicate that the total motility, progressive motility, LIN, STR, and WOB were the highest in the 20 mM supplementation group at the 2 h timepoint after thawing. Thus, the optimal concentration for glutamine supplementation in short-term boar semen extender during the holding time at 17 °C was 20 mM. Interestingly, at all of the time points after thawing, 20 mM glutamine supplementation exhibited the highest level of sperm viability and membrane integrity when compared to the CONTROL (0 mM) and other experimental dilution groups. Moreover, the acrosome integrity, mitochondrial activity, and capacitation status (F pattern) were significantly greater in the 20 mM supplementation group than the other groups at the 2 h timepoint after thawing. Conclusion Supplementation of glutamine at a concentration of 20 mM in a short-term semen extender (Bio Pig®) during the 17 °C holding time before cryopreservation, which had a standard freezing extender (9.0% glycerol and 1.9% Equex paste), could enhance the post-thaw sperm motility and quality parameters of cryopreservation.

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Determination of the optimal concentration of Minitube Equex paste for boar semen cryopreservation based on sperm motility characteristics

Cited by 3 publications

References 31 publications

Razlika između proteina sjemene plazme i proteina sperme za dobru i lošu sposobnost smrzavanja ejakulata nerasta

Razlika između proteina sjemene plazme i proteina sperme za dobru i lošu sposobnost smrzavanja ejakulata nerasta

Cryoprotectant effects of natural honey on spermatozoa quality of pre-freezing and frozen-thawed boar semen

Supplementation of glutamine in a short-term boar semen extender during 17°C holding time enhances post-thaw sperm quality for cryopreservation

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