Determination of the phytochemical screening, total polyphenols, flavonoids content, and antioxidant activity of soursop leaves (Annona muricata Linn.)

Abstract

Annona muricata Linn. (soursop) plant is a medicinal plant that has been utilized in folk medicine for treatment of infectious and inflammatory diseases. This study aimed to determine the phytochemicals content, total phenolic and flavonoid contents and antioxidant activities of A. muricata leaves. Antioxidant activity was performed via 2, 2’-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-diphenyl- 1-picrylhydrazyl (DPPH) radical scavenging assays. The phytochemical was extracted separately with distilled water and 96% ethanol by maceration. The results show that a wide variety of pharmacologically active compounds such as alkaloids, coumarins, tannins, flavonoids, carbohydrates, phenols, terpenoids, saponins were present in the leaves of A. muricata. The total phenolic and flavonoid content of ethanol extract in A. muricata leave achieved 609.08±5.82 mgGAE/g and 209.52±1.88 mgQE/g, respectively. The antioxidant activity of the ethanol extract of A. muricata was correlated with total phenolic and flavonoid content with values IC50 of 20.75±0.28 μg/ml, 12.84±0.21 μg/ml for DPPH and ABTS scavenging activity, respectively.

A Review on Annona muricata and Its Anticancer Activity

“…Alkaloids [ 35 ], megastigmanes, flavonol triglycosides [ 36 ], phenolics, cyclopeptides, and essential oils have all been found in various parts of the A. muricata ( Figure 1 ). Despite this, Annona species, including A. muricata , are a good source of reported acetogenin (AGE) compounds.…”

“…The leaves [ 96 , 97 , 98 , 99 , 100 ], pericarp [ 36 , 101 ], fruits [ 34 , 102 ], seeds [ 36 , 103 ], and roots [ 35 ] of A. muricata have ethnomedical uses. Roots of A. muricata have been employed in herbal therapy, whereas stem barks, stems, seeds, and leaves are the most common constituents in traditional medicinal decoctions [ 104 , 105 , 106 ].…”

Microencapsulation by Spray Drying and Antioxidant Activity of Phenolic Compounds from Tucuma Coproduct (Astrocaryum vulgare Mart.) Almonds

“…After, 2 mL of 10% saturated sodium carbonate was added and incubated in the dark for 2 h followed by measurement of absorbance at 760 nm. The TPC was calculated based on a standard curve of gallic acid (60–200 mg/mL) expressed as mg gallic acid equivalent (mg GAE/100) [ 38 ]. The analysis was also performed on the crude extract before drying in the same way that was performed on the microparticle.…”

“…Briefly, 800 μL of the solution obtained from the extraction of polyphenols content from the microparticles was mixed with 400 μL 2.5% aluminum chloride solution and incubated in the dark for 30 min followed by measurement of absorbance at 425 nm. The TFC was calculated based on a standard curve of quercetin (5–35 mg/mL) expressed as mg quercetin equivalent (mg QE/100 g) [ 38 ]. The analysis was also performed on the crude extract before drying in the same way that was performed on the microparticle.…”

Nutritional and Therapeutic Potential of Soursop

“…Its real task is to catalyze the conversion of acetyl-CoA and malonyl-CoA into palmitate (saturated fatty acid) in the Free radical scavenge activity. [32] Antihypertension and hyperuricemia properties Extracted with n-butanol Reduce the production of new uric acid and keep the blood pressure under control. [33] Induce apoptosis in cancer patients Flavonoids Potent inducer of apoptosis [26] presence of NADPH.…”