Determination of the population structure of common bean (Phaseolus vulgaris L.) accessions using lipoxygenase and resistance gene analog markers

Nemli, Seda; Kutlu, Burcu; Tanyolaç, Bahattin

doi:10.1016/j.bse.2015.01.016

Cited by 3 publications

(4 citation statements)

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“…The number of polymorphic bands varies from 2 for the RGA-RLRR and RGA-XLRR primer pairs to 7 for the RGA-NLRR primer pair with an average of 4.4 polymorphic bands. This agrees with a previous study on common beans (Nemli et al 2015) where the highest number of polymorphic bands for RGA-NLRR primer pair was 4 bands.…”

Section: Polymorphism Analysissupporting

confidence: 93%

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Genetic diversity and relationships among wild and cultivated Ficus carica L.: Usefulness of RGA markers

Haffar¹,

Baraket²,

Usai³

et al. 2023

J. OASIS AGRIC. SUSTAIN. DEV

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Disease resistance and the maintenance of genetic diversity in wild and cultivated populations are very important challenges to implement breeding program and markers assisted selection of Ficus carica L. facing climate change and its consequences. Resistance gene analogs (RGA) markers were used for variety discrimination and assessment of genetic structure and diversity of wild and cultivated Ficus carica L. species in Tunisia. The RGA markers were efficient and reliable markers for discriminating wild and cultivated fig. The high level of polymorphism (95.65) detected suggests the effectiveness of RGAs for both genetic fingerprinting and relationships assessment in wild and cultivated fig. The detected markers may represent candidate genes for disease resistance and could be further used to facilitate the identification of candidate genes and accelerate the genetic improvement of disease resistance in breeding programs of Ficus carica species.

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Section: Polymorphism Analysissupporting

confidence: 93%

“…The decrease in the number of polymorphic bands for RGA markers may result from the use of a limited number of RGAP primer pairs and its gene region targeting nature. Indeed, the RGA primers are designed based on coding conserved regions of the R genes (Nemli et al 2015).…”

Section: Cluster Analysismentioning

confidence: 99%

Genetic diversity and relationships among wild and cultivated Ficus carica L.: Usefulness of RGA markers

Haffar¹,

Baraket²,

Usai³

et al. 2023

J. OASIS AGRIC. SUSTAIN. DEV

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“…Common bean accessions #47 (Bandırma/Turkey, dwarf) and #82 (Havsa-Edirne/ Turkey, dwarf) in population II (Andean) were the most genetically similar (0.002) among all accessions. Notably, these values between high and low genetic diversity were broader than the genetic diversity level documented in earlier common bean studies (Kumar et al, 2008a(Kumar et al, , 2008bMaras et al, 2008;Nemli et al, 2015;Perseguini et al, 2015). This information obtained by genetic similarity could be acceptable as a permanent scientific foundation for the faster use of germplasm collections in plant improvement.…”

Section: Analysis Of Genetic Diversity and Population Structurementioning

confidence: 80%

Diversity and genetic analysis through DArTseq in common bean(Phaseolus vulgaris L.) germplasm from Turkey

Nemli¹,

Aşçioğul²,

Ateş³

et al. 2017

Turk J Agric For

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The common bean (Phaseolus vulgaris L.) is the most consumed food legume in the world and is a major source of dietary protein, carbohydrates, and valuable micronutrients, especially in developing countries. Diversity Arrays Technology (DArTseq), based on genome reduction with restriction enzymes, provides a rapid, high-throughput, and cost-effective tool capable of generating thousands of genotyped single nucleotide polymorphisms (SNPs) for a genome-wide analysis of genetic diversity. In this study, we aimed to characterize common bean accessions using SNPs detected by a DArTseq approach. A total of 43,018 SNPs were identified from 173 common bean accessions, including Andean and Mesoamerican genotypes. After filtering raw and redundant data, a total of 16,366 SNPs were considered for further analyses. According to population structure analysis, the genotypes were roughly divided into 2 gene pools of Andean and Mesoamerican types. Pairwise fixation index (Fst) values were calculated to resolve the differentiation between populations. This study demonstrated that discovering SNPs from the whole genome is a potential resource for identifying naturally diverse accessions and also the information could be used in breeding programs to develop new common bean varieties.

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“…The common bean (Phaseolus vulgaris) is one of the most important food crops for human consumption, mainly because it is a rich source of protein, complex carbohydrates, and micronutrients (Nemli et al, 2015). In Brazil, the common bean is a principal source of daily protein consumption (Toledo et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Research Article Infection and colonization of common bean by EGFP transformants of Fusarium oxysporum f. sp. phaseoli

Batista¹,

Leite²,

Nicoli³

et al. 2019

Genet. Mol. Res.

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Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. phaseoli (Fop) is one of the most important diseases of common bean and occurs in many regions of the world. However, there is little available information that could elucidate when and where the fungus attacks common bean plants. To study the colonization of common bean by this fungus, we transformed Fop Brazilian race 2 for egfp gene expression and used these fungal transformants to analyze the colonization process in common bean roots and stems. The combined use of driselase and lysing enzyme was sufficient for Fop mycelium protoplastization. The PEG-CaCl 2 transformation protocol allowed us to obtain stable transformed fungi, with colonies expressing the egfp gene. Regarding colonization by Fop transformants, the fungus grew intercellularly on the root hair epidermis at six days post-inoculation (DPI) and on parenchymal ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (4): gmr18370 R.O. Batista et al. 2 cells at 11 DPI on plants of the susceptible line. At 19 DPI, the fungus reached xylem vessels, blocking water and mineral passage in shoots, resulting in wilt and plant death at 25 DPI. Using fluorescence to mark the fungus revealed details of Fop colonization of common bean tissues.

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Determination of the population structure of common bean (Phaseolus vulgaris L.) accessions using lipoxygenase and resistance gene analog markers

Cited by 3 publications

References 50 publications

Genetic diversity and relationships among wild and cultivated Ficus carica L.: Usefulness of RGA markers

Genetic diversity and relationships among wild and cultivated Ficus carica L.: Usefulness of RGA markers

Diversity and genetic analysis through DArTseq in common bean(Phaseolus vulgaris L.) germplasm from Turkey

Research Article Infection and colonization of common bean by EGFP transformants of <i>Fusarium</i> <i>oxysporum</i> f. sp. <i>phaseoli</i>

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