Determination of the prevalence of Mycoplasma hominis and Ureaplasma species in Bacterial vaginosis patients in association with antibiotic resistance profile in Franceville, Gabon

Lendamba, Roméo Wenceslas; Nguema, Pierre Philippe Mbeang; Onanga, Richard; Landry-Erik, Mombo

doi:10.1016/j.micpath.2022.105528

Cited by 9 publications

(6 citation statements)

References 33 publications

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“…These studies suggested that Lactobacilli are a good indicator of the changes in the vaginal microenvironment as pathogenic bacteria. Moreover, in addition to the known BV related pathogens such as G. vaginalis and A. vaginae, a high proportion of genital mycoplasma (M. hominis and U. urealyticum) infections have been found in patients with BV, and some studies have shown that these genital mycoplasma infections are also related to the drug resistance of BV (Lendamba et al, 2022;Rosaŕio et al, 2023). However, none of the previous methods could reflect the changes in the levels of the representative species of five CSTs at the same time.…”

Section: Discussionmentioning

confidence: 99%

“…However, none of the previous methods could reflect the changes in the levels of the representative species of five CSTs at the same time. Therefore, we developed a general-purpose fluorescence PCR instrument-based BVLaB assay for key microorganisms in the five CSTs or microorganisms with high detection rate reported in previous studies (Kinoshita et al, 2014;Javed et al, 2019;Lendamba et al, 2022;Li et al, 2022;Carter et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

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Vaginal microbiota molecular profiling and diagnostic performance of artificial intelligence-assisted multiplex PCR testing in women with bacterial vaginosis: a single-center experience

Lu,

Li,

Chen

et al. 2024

Front. Cell. Infect. Microbiol.

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BackgroundBacterial vaginosis (BV) is a most common microbiological syndrome. The use of molecular methods, such as multiplex real-time PCR (mPCR) and next-generation sequencing, has revolutionized our understanding of microbial communities. Here, we aimed to use a novel multiplex PCR test to evaluate the microbial composition and dominant lactobacilli in non-pregnant women with BV, and combined with machine learning algorithms to determine its diagnostic significance.MethodsResidual material of 288 samples of vaginal secretions derived from the vagina from healthy women and BV patients that were sent for routine diagnostics was collected and subjected to the mPCR test. Subsequently, Decision tree (DT), random forest (RF), and support vector machine (SVM) hybrid diagnostic models were constructed and validated in a cohort of 99 women that included 74 BV patients and 25 healthy controls, and a separate cohort of 189 women comprising 75 BV patients, 30 intermediate vaginal microbiota subjects and 84 healthy controls, respectively.ResultsThe rate or abundance of Lactobacillus crispatus and Lactobacillus jensenii were significantly reduced in BV-affected patients when compared with healthy women, while Lactobacillus iners, Gardnerella vaginalis, Atopobium vaginae, BVAB2, Megasphaera type 2, Prevotella bivia, and Mycoplasma hominis were significantly increased. Then the hybrid diagnostic models were constructed and validated by an independent cohort. The model constructed with support vector machine algorithm achieved excellent prediction performance (Area under curve: 0.969, sensitivity: 90.4%, specificity: 96.1%). Moreover, for subjects with a Nugent score of 4 to 6, the SVM-BV model might be more robust and sensitive than the Nugent scoring method.ConclusionThe application of this mPCR test can be effectively used in key vaginal microbiota evaluation in women with BV, intermediate vaginal microbiota, and healthy women. In addition, this test may be used as an alternative to the clinical examination and Nugent scoring method in diagnosing BV.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Vaginal microbiota molecular profiling and diagnostic performance of artificial intelligence-assisted multiplex PCR testing in women with bacterial vaginosis: a single-center experience

Lu,

Li,

Chen

et al. 2024

Front. Cell. Infect. Microbiol.

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“…A combination of vaginal U. parvum and BV has been shown to significantly increase the risk for adverse pregnancy outcomes [32]. A study conducted by Lendamba et al 2022 found that the prevalence of genital mycoplasma infections such as M. hominis and Ureaplasma spp. are significantly high in women with bacterial vaginosis as 60.18% of the women were positive for BV and were genital mycoplasma carriers, including 5.19% pregnant women [33].…”

Section: Discussionmentioning

confidence: 99%

Mycoplasma hominis increases the risk for Ureaplasma parvum infection in Human immunodeficiency virus infected pregnant women

Nundlall,

Ngobese,

Singh

et al. 2024

J Infect Dev Ctries

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Introduction: Mycoplasma hominis and Ureaplasma parvum have been recently linked to sexually transmitted diseases and other conditions. There are a limited number of studies conducted on South African pregnant women that have assessed the prevalence and risk factors for genital mycoplasmas. Methodology: This study included 264 HIV infected pregnant women attending the King Edward VIII antenatal clinic in eThekwini, South Africa. DNA was extracted using the PureLink Microbiome kit and pathogens were detected using the TaqMan Real-time PCR assays. The statistical data analysis was conducted in a freely available Statistical Computing Environment, R software, version 3.6.3 using the RStudio platform. Results: The prevalence of M. hominis and U. parvum, was 215/264 (81.4%), and 203/264 (76.9%), respectively. In the M. hominis positive group, a significantly (p = 0.004) higher proportion, 80.5% tested positive for U. parvum infection when compared to 61.2% among the M. hominis negative. Of the U. parvum positive women, a significantly (p = 0.004) higher proportion of women (85.2%) tested positive for M. hominis when compared to 68.9% among the U. parvum negative. In the unadjusted and adjusted analysis, being M. hominis positive increased the risk for U. parvum by approximately 3 times more (p = 0.014) and 4-fold (p = 0.008), respectively. Conclusions: This study showed a significant link between M. hominis and U. parvum infection. To date, there are a limited number of studies that have investigated M. hominisbeing a risk factor for U. parvum infection. Therefore, the data presented in the current study now fills in this gap in the literature.

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“…This was comparable with the global reports on antibiotics susceptibility of GM. The published studies have reported that the antimicrobial resistance of mycoplasmas, especially against Macrolides, Fluoroquinolones and Tetracyclines antibiotics (Bebear et al, 2000;Lendamba, Mbeang Nguema, Onanga, & Mombo, 2022), has increased in previous Genital mycoplasmasin adult Bahraini females years. Therefore, minocycline and josamycin are recommended as a new primary choice in anti-mycoplasmas treatment (Lee & Yang, 2020).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of genital mycoplasmas and co-occurrence with Gardnerella vaginalis in high vaginal swabs from adult females in Bahrain: point-prevalence observational study

AL-Enezi

Saeed

Mahmood

et al. 2022

AGJSR

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PurposeBacterial vaginosis (BV) is quite common and linked with serious public health issues such as premature delivery and spread of sexually transmitted infections. The study aims to identify different genital mycoplasmas (GM) in high vaginal swabs (HVS) from adult females in Bahrain.Design/methodology/approachIn total, 401 HVS were collected and cultured on MYCOFAST® RevolutioN 2 test for identification and antibiotic susceptibility. Polymerase chain reaction (PCR) was performed for detection of Mycoplasma genitalium (Mg), Mycoplasma hominis (Mh) and Ureaplasma species. DNA-probe based detection for Gardnerella, Candida and Trichomonas was performed by BD Affirm Assay. Representative PCR amplicons were sequenced by Sanger sequencing.FindingsIn PCR, Ureaplasma sp. was the most common GM, followed by Mg and Mh; the prevalence being 21.2, 5.2 and 1.5%, respectively. On the contrary, 10.7% samples showed positivity for Ureaplasma urealyticum (Uu) and 1.7% for Mh in MYCOFAST® RevolutioN 2. The concordance rates between MYCOFAST® RevolutioN 2 and PCR for Mh and Ureaplasma sp. were 97.7 and 84%, respectively. Considering PCR as gold standard, sensitivity, specificity, positive predictive value, and negative predictive value of MYCOFAST® RevolutioN 2 were 33.3, 98.8, 28.6, 98.9 and 37.7, 96.5, 74.4, 85.2% for Mh and Ureaplasma sp., respectively. The Uu and Mh isolates showed antibiotic-resistance ranging from 53%–58% and 71%–86%, respectively.Research limitations/implicationsThe prevalence of Ureaplasma sp. was high. Significant co-occurrence of GM was noticed with BV. MYCOFAST® RevolutioN 2 had lower detection-rate than PCR, so a combination is suggested for wider diagnostic coverage.Practical implicationsThe research reflects on status of prevalence of GM in adult females in Bahrain, and their co-occurrence with bacterial vaginosis. Diagnostic approach with combination of tests is suggested for wider coverage. The research has epidemiologic, diagnostic, and therapeutic implications.Originality/valueThis is the first report from the Kingdom of Bahrain reflecting on burden of GM from this geographic location. The diagnostic efficacy of MYCOFAST® RevolutionN 2 test and polymerase chain reaction was evaluated for GM detection.

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Determination of the prevalence of Mycoplasma hominis and Ureaplasma species in Bacterial vaginosis patients in association with antibiotic resistance profile in Franceville, Gabon

Cited by 9 publications

References 33 publications

Vaginal microbiota molecular profiling and diagnostic performance of artificial intelligence-assisted multiplex PCR testing in women with bacterial vaginosis: a single-center experience

Vaginal microbiota molecular profiling and diagnostic performance of artificial intelligence-assisted multiplex PCR testing in women with bacterial vaginosis: a single-center experience

Mycoplasma hominis increases the risk for Ureaplasma parvum infection in Human immunodeficiency virus infected pregnant women

Prevalence of genital mycoplasmas and co-occurrence with Gardnerella vaginalis in high vaginal swabs from adult females in Bahrain: point-prevalence observational study

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