Determination of the pseudoephedrine content in pharmaceutical formulations and in biological fluids using a microbore HPLC system interfaced to a microfluidic chemiluminescence detector

Abstract

A novel automated precolumn derivatization followed by separation using liquid chromatography for the determination of pseudoephedrine (PSE) by a microfluidic chemiluminescence detector has been developed. An on-line derivatization procedure was utilized by converting PSE into a highly light emitting species in a Ru(bipy)3(2+)-peroxydisulphate chemiluminescence (CL) system by derivatizing it with a 1.0 M formaldehyde solution. The derivatized analyte was directly injected into a microbore high-performance liquid chromatography (HPLC) system coupled to an on-chip chemiluminescence detector. The newly developed highly selective, sensitive and fast HPLC-CL method was validated and successfully applied for the analysis of PSE in pharmaceutical formulations and a human urine sample. The selectivity of the method is not only due to the HPLC separation but is also due to the highly selective detection principle of the Ru(bipy)3(2+)-peroxydisulphate CL system used. There was no interference observed from the common preservatives and excipients used in pharmaceutical preparations, which did not show any significant CL signal. The retention time of PSE was less than 3 min, and the detection limits and quantification limits were found to be 5.7 and 26.0 µg L(-1), respectively.

Chemiluminescence selectivity enhancement in the on‐chip Ru(bpy)32+ system: The potential role of buffer type and pH in the determination of hydrochlorothiazide in combined formulations and human plasma

“…[26][27][28] This is because of relatively high mixing efficiency and reproducibility. Our group have been extensively studying the applications of a MF-CL detector for quantitative analysis of pharmaceutical formulations and biological samples [29][30][31][32] by combining it with flow injection, capillary HPLC [33] and micellar liquid chromatography [34] The unstable nature of Ru(bipy) 3…”

Luminol Chemiluminescence Catalyzed by Silver Nanoparticles for the Sensitive Determination of Penicillamine

“…The major advantages of CL have led to the extensive utilization of this technique for detection of analytes using flow injection analysis, sequential injection analysis, microfluidics, capillary electrophoresis and high performance liquid chromatography [28][29][30][31][32][33][34][35]. The major attraction to CL detection in these techniques stems from the high sensitivity and the simple instrumentation as only a photomultiplier tube is required, eliminating the use of expensive optics required by other techniques such as fluorescence spectroscopy.…”

Enhancing the chemiluminescence intensity of a KMnO4 formaldehyde system for estimating the total phenolic content in honey samples using a novel nanodroplet mixing approach in a microfluidics platform

“…The cost can be reduced significantly if a similar mixing efficiency can be achieved using a two‐layer design. Additionally, the size of the holes in the middle layer was very small and therefore the holes became prone to clogging and were difficult to clean …”