Determination of the Sensitivity of a Rapid
            <i>Escherichia coli</i>
            O157:H7 Assay for Testing 375-Gram Composite Samples

Tsai, Wan-Ling; Miller, Cynthia E.; Richter, Edward R.

doi:10.1128/aem.66.9.4149-4151.2000

Cited by 19 publications

(6 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This method is comparatively expensive due to the requirement of multiple antibodies and extensive instrumentation. Tsai and others (2000) reported a highly sensitive detection method for E. coli O157:H7 in ground beef by combining immunomagnetic separation and sandwich ELISA. This method detected 10 1 CFU/g of E. coli O157:H7 in ground beef, however the total time of detection was more than 30 h. The FT‐IR techniques reduced the time to detection by avoiding lengthy sample pretreatment and incubation steps.…”

Section: Resultsmentioning

confidence: 99%

Detection of E. coli O157:H7 from Ground Beef Using Fourier Transform Infrared (FT‐IR) Spectroscopy and Chemometrics

Davis¹,

Irudayaraj²,

Reuhs³

et al. 2010

Journal of Food Science

View full text Add to dashboard Cite

FT-IR spectroscopy methods for detection, differentiation, and quantification of E. coli O157:H7 strains separated from ground beef were developed. Filtration and immunomagnetic separation (IMS) were used to extract live and dead E. coli O157:H7 cells from contaminated ground beef prior to spectral acquisition. Spectra were analyzed using chemometric techniques in OPUS, TQ Analyst, and WinDAS software programs. Standard plate counts were used for development and validation of spectral analyses. The detection limit based on a selectivity value using the OPUS ident test was 10(5) CFU/g for both Filtration-FT-IR and IMS-FT-IR methods. Experiments using ground beef inoculated with fewer cells (10(1) to 10(2) CFU/g) reached the detection limit at 6 h incubation. Partial least squares (PLS) models with cross validation were used to establish relationships between plate counts and FT-IR spectra. Better PLS predictions were obtained for quantifying live E. coli O157:H7 strains (R(2)> or = 0.9955, RMSEE < or = 0.17, RPD > or = 14) and different ratios of live and dead E. coli O157:H7 cells (R(2)= 0.9945, RMSEE = 2.75, RPD = 13.43) from ground beef using Filtration-FT-IR than IMS-FT-IR methods. Discriminant analysis and canonical variate analysis (CVA) of the spectra differentiated various strains of E. coli O157:H7 from an apathogenic control strain. CVA also separated spectra of 100% dead cells separated from ground beef from spectra of 0.5% live cells in the presence of 99.5% dead cells of E. coli O157:H7. These combined separation and FT-IR methods could be useful for rapid detection and differentiation of pathogens in complex foods.

show abstract

Section: Resultsmentioning

confidence: 99%

Detection of E. coli O157:H7 from Ground Beef Using Fourier Transform Infrared (FT‐IR) Spectroscopy and Chemometrics

Davis¹,

Irudayaraj²,

Reuhs³

et al. 2010

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…However, a composite sampling plan, which combines randomly collected samples into one large composite sample (e.g., 375 g) and tests the entire composite at one time, can provide an economic advantage to the food industry (36). To better meet desired food safety objectives and to allow a more statistically representative sampling protocol, the results obtained for a 375-g composite sample were compared with those obtained for a single 25-g sample.…”

Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of a Phage Protein Ligand Assay with Real-Time PCR and a Reference Method for the Detection of Escherichia coli O157:H7 in Raw Ground Beef and Trimmings

Savoye

Feng

Rozand

et al. 2011

Journal of Food Protection

View full text Add to dashboard Cite

Enterohemorrhagic Escherichia coli O157:H7 is an important pathogen associated with infections caused by consumption of undercooked raw meat. Sensitive and rapid detection methods for E. coli O157:H7 are essential for the meat industry to ensure a safe meat supply. This study was conducted to compare the sensitivity of the VIDAS ultra performance E. coli test (ECPT UP) with a noncommercial real-time (RT) PCR method and the U.S. Department of Agriculture, Food Safety and Inspection Service (USDA-FSIS) reference method for detecting E. coli O157:H7 in raw ground beef. Optimal enrichment times and the efficacy of testing different types of raw meat, either as individual samples (25 g) or as composites (375 g), were examined. For 25-g samples of each type of raw ground beef tested, 6 h of enrichment was sufficient for both the VIDAS ECPT UP and RT-PCR methods, but for 375-g samples, 24 h of enrichment was required. Both the VIDAS ECPT UP and RT-PCR methods produced results similar to those obtained with the USDA-FSIS reference method after 18 to 24 h of enrichment. The primer specificity of the RT-PCR assay and the highly specific phage ligand used in the VIDAS ECPT UP for target recognition enabled the detection of low levels of E. coli O157:H7 in 25 g of various types of raw ground beef. The tests also allowed the detection of E. coli O157:H7 in composite raw ground beef and trimmings in samples of up to 375 g.

show abstract

“…0.1 µL of each urine sample received from the patients were spread on Eosine Methylene Blue (EMB) agar by spread plate method for the growth of bacteria and the culture plates were incubated in an inverted position at 37 °C under the aerobic condition for 24-48 hours [9]. After growth of organisms, the pink greenish metallic sheen was inoculated on nutrient agar medium for each discrete colony presented in the specimen to obtain well-isolated pure colonies incubated at 37 °C for 24 hours.…”

Section: Isolation and Identification Of Ecoli From Urine Samplesmentioning

confidence: 99%

Antibiotic susceptibility pattern of biofilm forming uropathogenic Escherichia coli isolated from UTI infected patients of Koshi Zonal Hospital in Biratnagar, Nepal

2018

View full text Add to dashboard Cite

Objectives: To investigate the prevalence and antibiotic resistance pattern of biofilm-forming Uropathogenic Escherichia coli (UPEC) from urine samples isolated from UTI infected patients of Koshi zonal hospital, Biratnagar.Methods: A total of 51 urine samples from urinary tract infected patients were collected from Koshi zonal hospital, Biratnagar in the period of July to August 2017. Following the isolation and identification of biofilm-forming uropathogenic Escherichia coli, antibiotic susceptibility test was performed by a modified Kirby-Bauer disc diffusion technique. The biofilm detection was done by Congo red agar method.Results: In the present study, 45% of the urine samples showed a predominant growth of E. coli, among which 70% of isolates exhibited positive biofilm formation. Biofilm forming isolates revealed 100%, 87.5%, 75%, 63% and 12.5% resistant to erythromycin, amoxicillin, cefotaxime, levofloxacin, and nitrofurantoin respectively. Approximately 87.5% of biofilm-forming isolates were found multi-drug resistant.Conclusion: The study revealed the major issue of UTI by E. coli which may be due to poor sanitation, not the proper cleanliness of genitals and unsafe sexual intercourse. Nitrofurantoin and levofloxacin were examined the most effective antibiotics for UPEC. BIBECHANA 16 (2019) 47-54

show abstract

Determination of the Sensitivity of a Rapid Escherichia coli O157:H7 Assay for Testing 375-Gram Composite Samples

Cited by 19 publications

References 17 publications

Detection of E. coli O157:H7 from Ground Beef Using Fourier Transform Infrared (FT‐IR) Spectroscopy and Chemometrics

Detection of E. coli O157:H7 from Ground Beef Using Fourier Transform Infrared (FT‐IR) Spectroscopy and Chemometrics

Comparative Evaluation of a Phage Protein Ligand Assay with Real-Time PCR and a Reference Method for the Detection of Escherichia coli O157:H7 in Raw Ground Beef and Trimmings

Antibiotic susceptibility pattern of biofilm forming uropathogenic Escherichia coli isolated from UTI infected patients of Koshi Zonal Hospital in Biratnagar, Nepal

Contact Info

Product

Resources

About