Determination of the small amount of proteins interacting with TiO2 nanotubes by AFM-measurement

Dong, Yihui; Ji, Xiaoyan; Laaksonen, Aatto; Cao, Wu; An, Rong; Lü, Linghong; Lü, Xiaohua

doi:10.1016/j.biomaterials.2018.11.013

Cited by 21 publications

(35 citation statements)

References 69 publications

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“…In the third part, to determine the EF of the SERS performance, the molecular force of Cyt c with TiO 2 was quantified through the combination of the adsorption capacity and adhesion force according to the method developed in our previous work. 42 The mechanism of the enhancement and molecular force was clarified and discussed, and the friction measurement was used to further verify the mechanism. The three different systems studied in this work are shown in Figure 1 (without ILs, with ILs in PBS, and with ILs immobilized on the TiO 2 surface, respectively).…”

Section: Resultsmentioning

confidence: 99%

“…On the basis of our previous work, detection of the trace amounts of proteins adsorbed on the solid film surfaces can be obtained by the AFM-quantified adhesion force. 42 …”

Section: Resultsmentioning

confidence: 99%

“…In this work, the mesoporous TiO 2 microparticles with different surface areas were used to provide the molecular force, and the provided molecular force is independent of the geometric structure of the materials as revealed in our previous work. 42 The results from the XRD measurements indicated that both the TiO 2 nanotube arrays and the mesoporous TiO 2 microparticles are the TiO 2 with the anatase crystals but different nanostructures (see Figure S6 ). Therefore, the molecular force of Cyt c with TiO 2 nanotube arrays is equal to that of Cyt c with mesoporous TiO 2 microparticles.…”

Section: Resultsmentioning

confidence: 99%

“… 40 , 41 To address this problem, in our previous work, an AFM-based approach was also proposed to determine the trace amounts of protein molecules on the substrates, thereby making it possible to determine the EF values accurately. 42 …”

Section: Introductionmentioning

confidence: 99%

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Hydrated Ionic Liquids Boost the Trace Detection Capacity of Proteins on TiO₂ Support

et al. 2021

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Trace detection based on surface-enhanced Raman scattering (SERS) has attracted considerable attention, and exploiting efficient strategies to stretch the limit of detection and understanding the mechanisms on molecular level are of utmost importance. In this work, we use ionic liquids (ILs) as trace additives in a protein-TiO 2 system, allowing us to obtain an exceptionally low limit of detection down to 10 –9 M. The enhancement factors (EFs) were determined to 2.30 × 10 4 , 6.17 × 10 4 , and 1.19 × 10 5 , for the three systems: one without ILs, one with ILs in solutions, and one with ILs immobilized on the TiO 2 substrate, respectively, corresponding to the molecular forces of 1.65, 1.32, and 1.16 nN quantified by the atomic force microscopy. The dissociation and following hydration of ILs, occurring in the SERS system, weakened the molecular forces but instead improved the electron transfer ability of ILs, which is the major contribution for the observed excellent detection. The weaker diffusion of the hydrated IL ions immobilized on the TiO 2 substrate did provide a considerably greater EF value, compared to the ILs in the solution. This work clearly demonstrates the importance of the hydration of ions, causing an improved electron transfer ability of ILs and leading to an exceptional SERS performance in the field of trace detection. Our results should stimulate further development to use ILs in SERS and related applications in bioanalysis, medical diagnosis, and environmental science.

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Section: Resultsmentioning

confidence: 99%

“…On the basis of our previous work, detection of the trace amounts of proteins adsorbed on the solid film surfaces can be obtained by the AFM-quantified adhesion force. 42 …”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hydrated Ionic Liquids Boost the Trace Detection Capacity of Proteins on TiO₂ Support

et al. 2021

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“…TiNTs have potential applications in several fields; they are found in biomaterials, gas sensors, adsorbents, heterogeneous catalysis ( photocatalysis, oxidation reactions, organic synthesis, biomass,…), biological activities to remove bacteria from water, solar cells, Li battery production, and many others. In particular, TiNTs have been used as a catalytic support for different active species (metals, oxides and sulfides).…”

Section: Introductionmentioning

confidence: 99%

Study of 1D Titanate‐Based Materials –New Modification of the Synthesis Procedure and Surface Properties‐Recent Applications

Ameur

Bachir

2020

ChemistrySelect

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This paper represents a general study of titanate nanotubes (TiNTs) which are among the most important materials in the field of catalysis. This research work covers more than 100 publications about the formulation, characterisation and most recent applications of TiNTs. TiNTs can be prepared by different methods, namely the anodic deposition, sol-gel template synthesis and alkaline hydrothermal treatment. The present study focuses on the preparation of this tubular structure by alkaline treatment, using the latest and most recent processes that have been introduced in this method (stirring, acid nature, annealing temperature, etc). The surface properties, such as the point of zero charge (P.Z.C), acid-base nature and light absorption, of TiNTs are also evoked. In the end, some recent and important TiNTs applications in the field of renewable energies are reported (H 2 production and methanation). In addition, NO reduction, CO oxidation, photocatalysis and organic synthesis are also mentioned. Therefore, it may be said that this paper is a structured study about TiNTs for the purpose of making the reader aware of their importance, understand their synthesis, and know their scope of applications. To conclude, the most important data, acquired through various characterization techniques, are provided.[a] Dr.

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AFM Study of pH‐Dependent Adhesion of Single Protein to TiO₂ Surface

Dong

Laaksonen

Cao

et al. 2019

Adv Materials Inter

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The effect of pH‐induced electrostatic conditions on the molecular interaction force of a single lysozyme molecule with TiO2 is investigated using atomic force microscopy (AFM). The force between the charged or neutral lysozyme molecule and the TiO2 surface is measured at different pH from 3.6 to 10.8. It is found to be directly proportional to the contact area, given by an effective diameter of the lysozyme molecule, and is further qualitatively verified by the AFM‐measured friction coefficients. The results of the Derjaguin–Landau–Verwey–Overbeek theory show that the pH can change the surface charge densities of both lysozyme and TiO2, but the molecular interaction force at different pH is only dependent on the pH‐induced effective diameter of lysozyme. The molecular interaction forces, quantified at the nanoscale, can be directly used to design high‐performance liquid chromatography measurements at macroscale by tuning the retention time of a protein under varied pH conditions. They can also be applied to develop a model for predicting and controlling the chromatographic separations of proteins.

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Determination of the small amount of proteins interacting with TiO2 nanotubes by AFM-measurement

Cited by 21 publications

References 69 publications

Hydrated Ionic Liquids Boost the Trace Detection Capacity of Proteins on TiO₂ Support

Hydrated Ionic Liquids Boost the Trace Detection Capacity of Proteins on TiO₂ Support

Study of 1D Titanate‐Based Materials –New Modification of the Synthesis Procedure and Surface Properties‐Recent Applications

AFM Study of pH‐Dependent Adhesion of Single Protein to TiO₂ Surface

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Determination of the small amount of proteins interacting with TiO2 nanotubes by AFM-measurement

Cited by 21 publications

References 69 publications

Hydrated Ionic Liquids Boost the Trace Detection Capacity of Proteins on TiO2 Support

Hydrated Ionic Liquids Boost the Trace Detection Capacity of Proteins on TiO2 Support

Study of 1D Titanate‐Based Materials –New Modification of the Synthesis Procedure and Surface Properties‐Recent Applications

AFM Study of pH‐Dependent Adhesion of Single Protein to TiO2 Surface

Contact Info

Product

Resources

About

Hydrated Ionic Liquids Boost the Trace Detection Capacity of Proteins on TiO₂ Support

Hydrated Ionic Liquids Boost the Trace Detection Capacity of Proteins on TiO₂ Support

AFM Study of pH‐Dependent Adhesion of Single Protein to TiO₂ Surface