This paper presents an isothermal, single-phase model for direct ethanol fuel cells. The ethanol electrooxidation reaction is described using a detailed kinetic model that is able to predict anode polarization and product selectivity data. The anode kinetic model is coupled to a one-dimensional (1D) description for mass and charge transport across the membrane electrode assembly, which accounts for the mixed potential induced in the cathode catalyst layer by the crossover of ethanol and acetaldehyde. A simple 1D advection model is used to describe the spatial variation of the concentrations of the different species as well as the output and parasitic current densities along the flow channels. The proposed 1D+1D model includes two adjustable parameters that are fitted by a genetic algorithm in order to reproduce previous experimental data. The calibrated model is then used to investigate the consumption of ethanol and the production, accumulation and consumption of acetaldehyde along the flow channels, which yields the product selectivity at different channel cross-sections. A parametric study is also presented for varying ethanol feed concentrations and flow rates. The results obtained under ethanol starvation conditions highlight the role of acetaldehyde as main free intermediate, which is first produced and later consumed once ethanol is fully depleted. The detailed kinetic description of the ethanol oxidation reaction enables the computation of the four efficiencies (i.e., theoretical, voltage, faradaic, end energy utilization) that characterize the operation of direct ethanol fuel cells, thus allowing to present overall fuel efficiency vs. cell current density curves for the first time.