Determination of the structure and molecular weights of the exopolysaccharide produced by Lactobacillus acidophilus 5e2 when grown on different carbon feeds

Laws, Andrew P.; Chadha, Marcus J.; Chacon-Romero, Mariana; Marshall, Valerie M.; Maqsood, Mohammed

doi:10.1016/j.carres.2007.10.028

Cited by 30 publications

(17 citation statements)

References 24 publications

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“…If the configuration is α, the chemical shift is larger than β [34]. Furthermore, the α anomeric configuration shows 3 J 1,2 coupling constants of less than 4 Hz whereas β configuration presents coupling constants greater than 7.5 Hz [35]. Anomeric carbon signals resonate between 90 and 112 ppm at 13 C-NMR experiments.…”

Section: Configuration Of the Glycosidic Bondmentioning

confidence: 99%

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

2012

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Abstract:Polysaccharides from mushrooms have attracted a great deal of attention due to the many healthy benefits they have demonstrated, such as immunomodulation, anticancer activity, prevention and treatment of cardiovascular diseases, antiviral and antimicrobial effects, among others. Isolation and purification of polysaccharides commonly involve several steps, and different techniques are actually available in order to increase extraction yield and purity. Studies have demonstrated that the molecular structure and arrangement significantly influence the biological activity; therefore, there is a wide range of analytical techniques for the elucidation of chemical structures. Different polysaccharides have been isolated from mushrooms, most of them consisting of β-linked glucans, such as lentinan from Lentinus edodes, pleuran from Pleurotus species, schizophyllan from Schizophyllum commune, calocyban from Calocybe indica, or ganoderan and ganopoly from Ganoderma lucidum. This article reviews the main methods of polysaccharide isolation and structural characterization, as well as some of the most important polysaccharides isolated from mushrooms and the healthy benefits they provide.

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Section: Configuration Of the Glycosidic Bondmentioning

confidence: 99%

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

2012

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“…5e2 the structure of which has been previously described 23 and reproduced here ( acidophilus sp. 5e2 EPS as occurred with L. helveticus sp.…”

Section: Figures 5a and 5b Herementioning

confidence: 99%

“…In addition, its immunomodulatory potential is compared with that of an EPS isolated from Lactobacillus acidophilus sp. 5e2 the structure of which has been previously described 23 . H-HSQC-TOCSY spectra (not shown) and the assignments are tabulated in Table 1.…”

Section: Introductionmentioning

confidence: 99%

The structure and immunomodulatory activity on intestinal epithelial cells of the EPSs isolated from Lactobacillus helveticus sp. Rosyjski and Lactobacillus acidophilus sp. 5e2

Patten

Leivers

Chadha

et al. 2014

Carbohydrate Research

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“…Lactic acid bacteria (LAB) can produce a large structural variety of EPS and oligosaccharides from glucose that differing in size, molecular organization, chemical composition, structure, and genetic determinants (41) through the activity of glycansucrase and glycosyltransferase (GTF) enzymes. The chemical composition of repeating units of EPS produced by microorganisms has been determined by nuclear magnetic resonance (NMR) spectroscopy (31,35,48,51) through the application of 1 H and 13 C NMR mono-and bidimensional spectra (19). An updated review of composition, structure, and yield of homopolysaccharides (HoPS) and heteropolysaccharides (HePS) synthesized by LAB strains determined by NMR spectroscopy was previously reported (50).…”

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confidence: 99%

Polyphasic Screening, Homopolysaccharide Composition, and Viscoelastic Behavior of Wheat Sourdough from a Leuconostoc lactis and Lactobacillus curvatus Exopolysaccharide-Producing Starter Culture

Palomba

Cavella

Torrieri

et al. 2012

Appl Environ Microbiol

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ABSTRACTAfter isolation from different doughs and sourdoughs, 177 strains of lactic acid bacteria were screened at the phenotypic level for exopolysaccharide production on media containing different carbohydrate sources. Two exopolysaccharide-producing lactic acid bacteria (Lactobacillus curvatus69B2 andLeuconostoc lactis95A) were selected through quantitative analysis on solid media containing sucrose and yeast extract. The PCR detection of homopolysaccharide (gtfandlev) and heteropolysaccharide (epsA,epsB,epsDandepsE, andepsEFG) genes showed different distributions within species and strains of the lactic acid bacteria studied. Moreover, in some strains both homopolysaccharide and heteropolysaccharide genes were detected. Proton nuclear magnetic resonance spectra suggest thatLactobacillus curvatus69B2 andLeuconostoc lactis95A produced the same exopolysaccharide, which was constituted by a single repeating glucopyranosyl unit linked by an α-(1→6) glycosidic bond in a dextran-type carbohydrate. Microbial growth, acidification, and viscoelastic properties of sourdoughs obtained by exopolysaccharide-producing and nonproducing lactic acid bacterial strains were evaluated. Sourdough obtained after 15 h at 30°C with exopolysaccharide-producing lactic acid bacteria reached higher total titratable acidity as well as elastic and dissipative modulus curves with respect to the starter not producing exopolysaccharide, but they showed similar levels of pH and microbial growth. On increasing the fermentation time, no difference in the viscoelastic properties of exopolysaccharide-producing and nonproducing samples was observed. This study suggests that dextran-producingLeuconostoc lactis95A andLactobacillus curvatus69B2 can be employed to prepare sourdough, and this would be particularly useful to improve the quality of baked goods while avoiding the use of commercially available hydrocolloids as texturizing additives.

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Determination of the structure and molecular weights of the exopolysaccharide produced by Lactobacillus acidophilus 5e2 when grown on different carbon feeds

Cited by 30 publications

References 24 publications

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

The structure and immunomodulatory activity on intestinal epithelial cells of the EPSs isolated from Lactobacillus helveticus sp. Rosyjski and Lactobacillus acidophilus sp. 5e2

Polyphasic Screening, Homopolysaccharide Composition, and Viscoelastic Behavior of Wheat Sourdough from a Leuconostoc lactis and Lactobacillus curvatus Exopolysaccharide-Producing Starter Culture

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