Determination of the threshold dose distribution in photodynamic action from in vitro experiments

Faria, Clara Maria Gonçalves de; Inada, Natália Mayumi; Kurachi, Cristina; Bagnato, Vanderlei Salvador

doi:10.1016/j.jphotobiol.2016.06.041

Cited by 13 publications

(7 citation statements)

References 55 publications

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“… 11 PpIX accumulation showed a decreasing pattern when the ALA concentration exceeded the optimal threshold (OPT). 22 This phenomenon may be related to pH conditions, where maintaining a pH of 7.0 to 7.6 appeared to be conducive to increasing PpIX levels within the cells. 23 , 24 Taken together, these results highlight the exceptional nanomaterial properties of ANPs as a versatile drug delivery system.…”

Section: Discussionmentioning

confidence: 99%

Effects of Photodynamic Therapy Using 5 -Aminolevulinic Acid (ALA) Loaded Acrylic Nanoparticles (ANPs) on HaCaT Cells

Ge,

Zhao,

Liu

et al. 2024

CCID

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Objective ALA-PDT (5-aminolevulinic acid photodynamic therapy) is a central modality in the treatment of skin diseases. Increasing the bioavailability of ALA remains a critical issue. With this in mind, our study explores a novel route of ALA delivery by loading acrylic nanoparticles (ANPs). Methods ALA-ANPs were synthesized by emulsion polymerisation and characterised by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). The effects of ALA-ANPs on HaCaT cell line were evaluated, including characteristics, morphological changes, protoporphyrin IX (PpIX) fluorescence kinetics, reactive oxygen species (ROS) levels, mitochondrial membrane potential and ki67 expression in these cells. Results The ANPs had uniform sizes, smooth surfaces and excellent light transmittance, with diameters of 150–200 nm. In contrast, the ALA - ANPs had uneven surfaces and poor light transmittance, with diameters of 220–250 nm. During 12 hours of co-incubation of HaCaT cells with ALA, the intracellular accumulation of PpIX increased over time. Notably, after 6 hours of incubation, PpIX levels induced by 1.81 mg/mL ALA-ANPs exceeded those induced by 1.0 mM ALA ( p < 0.01 ). CCK-8 results showed a positive correlation between PDT-induced inhibition of HaCaT cell proliferation and ALA concentration when ALA concentration remained below 2.0 mM. Compared to the 1.0 mM ALA group, the 1.81 mg/mL ALA-ANPs group showed decreased mitochondrial membrane potential, ki67 immunofluorescence intensity and cell proliferation. In contrast, ROS levels were significantly increased in the 1.81 mg/mL ALA-ANPs group (p < 0.01). Conclusion Loading ANPs provide improved stability and potency for ALA. The ALA-ANPs-PDT approach has superior inhibitory effects on HaCaT proliferation in vitro.

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Section: Discussionmentioning

confidence: 99%

Effects of Photodynamic Therapy Using 5 -Aminolevulinic Acid (ALA) Loaded Acrylic Nanoparticles (ANPs) on HaCaT Cells

Ge,

Zhao,

Liu

et al. 2024

CCID

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“…4 In photodynamic therapy (PDT), reactive oxygen species produced by the PS decrease the microbial load of the canal. [5][6][7][8] However, the PS residues in the root canal system can cause a smear layer, which causes microbial microleakage and poor adhesion of root filling material to root dentin. Thus, a final rinse would be required to eliminate the smear layer.…”

Section: Introductionmentioning

confidence: 99%

Effect of Photodynamic Therapy on the Pushout Bond Strength of Resin-Based and Calcium Silicate-Based Endodontic Sealers

Zadsirjan,

Asnaashari,

Yazdani

et al. 2023

J Lasers Med Sci

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Introduction: This study aimed to assess the effect of photodynamic therapy (PDT) on the pushout bond strength (PBS) of resin-based and calcium silicate-based endodontic sealers. Methods: Forty single-canal teeth were assigned to four groups. After root canal preparation by the crown-down technique and smear layer removal, groups 1 and 2 served as the control group, and groups 3 and 4 underwent PDT by using a low-level diode laser as the light source and methylene blue as the photosensitizer (MB). The root canals in groups 1 and 3 were filled with AH26 sealer while the root canals in groups 2 and 4 were filled with Endoseal TCS. The samples were sectioned at 4, 8, and 12 mm from the apex tip (1mm thick slices). The PBS test was performed, and the mode of failure was determined in the coronal, middle, and apical thirds. The data were analyzed by the Kruskal-Wallis, Friedman, and Dunn tests. Results: No significant difference was noted in the PBS of resin-based and calcium silicate-based sealers in the apical, middle, or coronal thirds in the application of PDT (P>0.05). The PBS of AH26 was significantly higher than that of Endoseal TCS (P<0.05). Cohesive failure was the dominant mode of failure in the AH26 groups, while mixed failure was the dominant failure mode in the Endoseal TCS groups. Conclusion: PDT had no significant effect on the PBS of resin-based and calcium silicate-based sealers.

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“…In photodynamic therapy, the threshold dose is the amount of light required to cause irreversible cell damage. 99 In a population, the variability between individuals leads to a distribution in the threshold values which could explain the behavior seen in Figure 8. On the other hand, the same protocol had no significant impact on the viability of any of the cell lines tested, thus not allowing a median threshold dose to be estimated.…”

Section: Discussionmentioning

confidence: 93%

“…The other publications that used ICG and infrared light on cell lines found cytotoxicity but used concentrations of ICG that were much higher. [98][99] In the case of Engel et al, rather than measuring the photodynamic effect, they were interested in the toxicity of the products of the photodegradation of ICG, which was significant for RPE cells. However, in our case, there is no evidence of that occurring.…”

Section: Discussionmentioning

confidence: 99%

Pulmonary antimicrobial photodynamic therapy and the role of the lung surfactant

Kassab¹

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Determination of the threshold dose distribution in photodynamic action from in vitro experiments

Cited by 13 publications

References 55 publications

Effects of Photodynamic Therapy Using 5 -Aminolevulinic Acid (ALA) Loaded Acrylic Nanoparticles (ANPs) on HaCaT Cells

Effects of Photodynamic Therapy Using 5 -Aminolevulinic Acid (ALA) Loaded Acrylic Nanoparticles (ANPs) on HaCaT Cells

Effect of Photodynamic Therapy on the Pushout Bond Strength of Resin-Based and Calcium Silicate-Based Endodontic Sealers

Pulmonary antimicrobial photodynamic therapy and the role of the lung surfactant

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