Determination of the time course of capacitation in mouse spermatozoa using a chlortetracycline fluorescence assay

Biphasic Effect of GABA on Rat Sperm Acrosome Reaction: Involvement of GABA A and GABA B Receptors

“…GABA B receptors are localized in the dorsal region of the acrosome of rat spermatozoa [15], while the GABA A receptors are located principally on the ventral region of the acrosome (unpublished data). AR in rat spermatozoa is characterized by the disappearance of the¯uorescent CTC staining of the head ( Figure 1C), similar to the patterns observed with mouse sperm [31]. The present ®ndings support the hypothesis that GABA and progesterone trigger AR in rat sperm by activating the GABA A receptor pathway.…”

Comparison of Capacitation and Fertilizing Ability of BALB/c and ICR Mouse Epididymal Spermatozoa. Analysis by In Vitro Fertilization with Cumulus-intact and Zona-free Mouse Eggs.

“…In the present study, in vitro capacitation rates of epididymal spermatozoa from ICR mice at different incubation time points in medium supplemented with BSA (0.4%) were consistent with rates reported by Ward and Story [11]. After 1 and 2 h of preincubation, however, the capacitation rates of BALB/c spermatozoa did not increase to the same degree as rates for ICR spermatozoa.…”

“…Fluorescence assay was performed with the method originally developed by Ward and Storey [11] and slightly modified by Xian et al [12]. Chlortetracycline (CTC; C-4881, Sigma) at a 1 mM concentration was dissolved in a chilled buffer of 20 mM Tris, 130 mM NaCl, and 5 mM cysteine.…”

Chromosome analysis of mouse zygotes produced by intracytoplasmic injection of spermatozoa exposed to acrosome reaction inducing agents methyl-β-cyclodextrin and calcium ionophore A23187

“…However, the aberration rate was reduced when spermatozoa were incubated for 2 h or more in bicarbonate-buffered TYH medium, which can effectively induce the capacitation and acrosome reaction in mouse spermatozoa [11]. Furthermore, when sperm incubation was carried out in hepes and phosphate-buffered media that never induce the capacitation and acrosome reaction, the chromosome aberration rate in resultant ICSI zygotes was raised in a time-dependent manner, but the time-dependent increase in chromosome aberrations disappeared when testicular spermatozoa with a low content of cholesterol in the plasma membrane were used [12].…”