Determination of Theobromine, Theophylline, and Caffeine in Various Food Products Using Derivative UV-Spectrophotometric Techniques and High-Performance Liquid Chromatography

Abuirjeie, Mustafa A.; El-Din, M. Sharaf; Mahmoud, Ibrahim I.

doi:10.1080/10826079208018811

Cited by 20 publications

(11 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reversedphase HPLC with UV-detection has been successfully applied for the separation and determination of these compounds in a range of foods, beverages and biological fluids [9][10][11][17][18][19]. In some cases amperometric detection [20][21][22] or cathodic stripping voltammetry [16] was preferred, because of the higher sensitivity and selectivity of these detection techniques.…”

Section: Introductionmentioning

confidence: 99%

“…Caffeine, theobromine and theophylline were analysed by a Kjeldahl method [3], UV-spectrometry [4], thin-layer chromatography [5,6], gas chromatography [7], potentiometric titrations [8] and HPLC [9][10][11]. Adenine was determined by adsorptive stripping voltammetry [12], gas chromatography [13], capillary zone electrophoresis [14] and HPLC [15,16].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of adenine, caffeine, theophylline and theobromine by HPLC with amperometric detection

Meyer

Ngiruwonsanga

Henze

1996

Analytical and Bioanalytical Chemistry

View full text Add to dashboard Cite

A relatively simple method for quantifying caffeine, theobromine, theophylline and adenine by HPLC with amperometric detection was developed. A C(18)-column and an isocratic elution with phosphate buffer pH 3.5/methanol (90 : 10) were employed for the chromatographic separation of the investigated compounds. The optimal detection potential was +1.4 V. The limits of detection were 0.4 ng for adenine, 1 ng for theophylline and 2.5 ng for caffeine and theobromine. The method was applied to the determination of these purine alkaloids in beverages, tea, coffee and cacao. The determination was carried out directly or after solid-phase extraction.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of adenine, caffeine, theophylline and theobromine by HPLC with amperometric detection

Meyer

Ngiruwonsanga

Henze

1996

Analytical and Bioanalytical Chemistry

View full text Add to dashboard Cite

show abstract

“…Among these methods, RP-HPLC is one of the most commonly used technologies. Abuirjeie et al [14] used LC equipped with derivative UV-spectrophotometry to simultaneously determine xanthines in selected food products. Meyer et al [15] simultaneously determined caffeine, theophylline, and theobromine in different food products by LC with amperometric detection.…”

Section: Introductionmentioning

confidence: 99%

Quantitative capillary electrophoresis and its application in analysis of alkaloids in tea, coffee, coca cola, and theophylline tablets

Zhou

et al. 2009

J of Separation Science

View full text Add to dashboard Cite

Original PaperQuantitative capillary electrophoresis and its application in analysis of alkaloids in tea, coffee, coca cola, and theophylline tablets A quantitative CE (qCE) system with high precision has been developed, in which a 4-port nano-valve was isolated from the electric field and served as sample injector. The accurate amount of sample was introduced into the CE system with high reproducibility. Based on this system, consecutive injections and separations were performed without voltage interruption. Reproducibilities in terms of RSD lower than 0.8% for retention time and 1.7% for peak area were achieved. The effectiveness of the system was demonstrated by the quantitative analysis of caffeine, theobromine, and theophylline in real samples, such as tea leaf, roasted coffee, coca cola, and theophylline tablets.

show abstract

“…1 It is one of the main metabolites of caffeine. 2 Caffeine is a major constituent of many common non-prescription drug preparations, including cold remedies, diet pills diuretics and other stimulants. The efficiency and toxicity of theophylline may be modified by many factors; 3 excessive intake of these two xanthine derivatives cause many undesired side effects and give rise to symptoms, such as tremor and tachycardia.…”

Section: Theophylline (Dimethylxanthine)mentioning

confidence: 99%

Simultaneous Determination of Theophylline and Caffeine by Proton Magnetic Resonance Spectroscopy Using Partial Least Squares Regression Techniques

et al. 2003

View full text Add to dashboard Cite

A 1 H-NMR procedure based on an analysis of its data by a multivariate calibration method was conducted for the simultaneous determination of theophylline and caffeine in synthetic and real samples. Partial least squares regression (PLS) was chosen as the calibration method. The methyl signals of theophilline at 3.36 and 3.54 ppm that overlapped with those of caffeine were significant characteristics which were employed in this study for their analyses. The proposed method was successfully applied to recovery studies of theophylline and caffeine from real tablet samples.

show abstract

Determination of Theobromine, Theophylline, and Caffeine in Various Food Products Using Derivative UV-Spectrophotometric Techniques and High-Performance Liquid Chromatography

Cited by 20 publications

References 13 publications

Determination of adenine, caffeine, theophylline and theobromine by HPLC with amperometric detection

Determination of adenine, caffeine, theophylline and theobromine by HPLC with amperometric detection

Quantitative capillary electrophoresis and its application in analysis of alkaloids in tea, coffee, coca cola, and theophylline tablets

Simultaneous Determination of Theophylline and Caffeine by Proton Magnetic Resonance Spectroscopy Using Partial Least Squares Regression Techniques

Contact Info

Product

Resources

About