Determination of Therapeutic and Safety Effects of <i>Zygophyllum coccineum</i> Extract in Induced Inflammation in Rats

The emergence of bacteria that are resistant to several antibiotics has represented a serious hazard to human health globally. Bioactive metabolites from medicinal plants have a wide spectrum of therapeutic possibilities against resistant bacteria. Therefore, this study was performed to investigate the antibacterial efficacy of various extracts of three medicinal plants as Salvia officinalis L., Ziziphus spina-christi L., and Hibiscus sabdariffa L. against pathogenic Gram-negative Enterobacter cloacae (ATCC13047), Pseudomonas aeruginosa (RCMB008001), Escherichia coli (RCMB004001), and Gram-positive Staphylococcus aureus (ATCC 25923), bacteria using the agar-well diffusion method. Results revealed that, out of the three examined plant extracts, the methanol extract of H. sabdariffa L. was the most effective against all tested bacteria. The highest growth inhibition (39.6 ± 0.20 mm) was recorded against E. coli. Additionally, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the methanol extract of H. sabdariffa were detected in the case of all tested bacteria. Moreover, an antibiotic susceptibility test revealed that all tested bacteria showed multidrug resistance (MDR). While 50% of tested bacteria were sensitive and 50% were intermediately sensitive to piperacillin/tazobactam (TZP) based on the inhibition zone but still less than the extract. Synergistic assay demonstrated the promising role of using a combination of H. sabdariffa L. and (TZP) against tested bacteria. A surface investigation using a scanning electron microscope of the E. coli treated with TZP, extract, or a combination of the two revealed extremely considerable bacterial cell death. In addition, H. sabdariffa L. has a promising anticancer role versus Caco-2 cells with IC50 of 17.51 ± 0.07 µg/mL and minimal cytotoxicity upon testing versus Vero cells with CC50 of 165.24 ± 0.89 µg/mL. Flow cytometric analysis confirmed that H. sabdariffa extract significantly increased the apoptotic rate of Caco-2-treated cells compared to the untreated group. Furthermore, GC-MS analysis confirmed the existence of various bioactive components in the methanol hibiscus extract. Utilizing molecular docking with the MOE-Dock tool, binding interactions between n-Hexadecanoic acid, hexadecanoic acid-methyl ester, and oleic acid, 3-hydroxypropyl ester were evaluated against the target crystal structures of E. coli (MenB) (PDB ID:3T88) and the structure of cyclophilin of a colon cancer cell line (PDB ID: 2HQ6). The observed results provide insight into how molecular modeling methods might inhibit the tested substances, which may have applications in the treatment of E. coli and colon cancer. Thus, H. sabdariffa methanol extract is a promising candidate to be further investigated for developing alternative natural therapies for infection treatment.

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“…stock solution, and they were kept at room temperature for ten minutes. Flow cytometry was utilized for analysis [ 39 , 40 ].…”

Section: Methodsmentioning

confidence: 99%

GC-MS Analysis, Antibacterial, and Anticancer Activities of Hibiscus sabdariffa L. Methanolic Extract: In Vitro and In Silico Studies

et al. 2023

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“…The analytical variation was calculated using the GraphPad Prism 5 program (San Diego, CA, USA). To find differences between groups where P≤0.05 is considered significant, one-way analysis (ANOVA) was applied, followed by Tukey's post-hoc test [38][39][40].…”

Section: Statistical Variationsmentioning

confidence: 99%

Estimation of the Antibacterial and Anti-Tumor Impacts of Soy Milk and Ecofriendly Myco-Manufactured Zinc Oxide Nanomaterials. <i>In vitro</i> Appraisal

Helmy,

Amin,

Alqhtani

et al. 2024

Pol. J. Environ. Stud.

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The necessity for the creation of safe, dependable, biocompatible, and efficient methods to make nanoparticles drives an increasing number of researchers to consider using biological systems as potential eco-friendly nanofactories. Fungi have an innate ability to reduce and oxidize metal ions into metal oxide nanoparticles, thus behaving as nanofactories. In the present study, Trichoderma harzianum was used to prepare ZnO nanoparticles (ZnO NPS). ZnO NPS showed a peak at 370 nm upon testing using UV spectrophotometer and percentages of zinc(72.0±0.2 %) and oxygen(28.0 ±0.3) upon analysis using Edx with an irregular shape, 16.5 nm size and characterized XRD pattern. Soymilk as well as the prepared ZnONPs were investigated either separately or in their mixture (1:1) for their antibacterial and anti-tumor effects. The mixture showed a promising antibacterial impact on Enterococcus faecalis (ATCC29212) and Escherichia coli (ATCC25922) which was confirmed using a transmission electron microscope. ZnONPs mixed with soymilk showed a promising anti-tumor action towards human colorectal adenocarcinoma cell line Caco-2 through boosting apoptotic rate, which was detected by flow cytometry. The current report highlights the possibility of mixing eco-friendly biosynthesized nanoparticles with natural products to enhance their biomedical impact with minimal toxicity as possible innovative pharmaceutical applications.

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“…Through infusion experiments, the ESI-MS/MS parameters were altered to create the most deprotonated molecules and the most effective generation of unique fragment ions for all compounds. MS-DIAL V. 3.70 software was used to identify and calculate the relative percentage of molecules [23].…”

Section: Ultrastructural Changes Brought On By H Perforatummentioning

confidence: 99%

In Vitro Antibacterial and Phytochemical Screening of Hypericum perforatum Extract as Potential Antimicrobial Agents against Multi‐Drug‐Resistant (MDR) Strains of Clinical Origin

Sherif

Elshikh

Abdel‐Aziz

et al. 2023

BioMed Research International

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Background. The perennial plant Hypericum perforatum is widely distributed around the world. It has been used for many years in conventional medicine to treat a variety of illnesses, including stress, mild to moderate depression, and minor injuries. This study examined the antimicrobial activity of the H. perforatum total extract and its fractions (n-hexane, ethyl acetate, chloroform, and aqueous) against multi-drug-resistant (MDR) isolates that were gathered from clinical samples, including methicillin-resistant Staphylococcus aureus (MRSA), Enterococcus faecalis, Escherichia coli, and Klebsiella pneumonia. Materials and Methods. Aerial parts of H. perforatum were collected and extracted using various solvents and were tested versus different isolated bacterial species. The inhibition zone of tested extracts was detected using an agar diffusion assay, and MICs were measured. Phytochemical analysis of promising H. perforatum extract was done using LC-ESI-MS/MS. Ultrastructure examination for the most altered bacteria used transmission electron microscopy. Antioxidant assays were done using DPPH and ABTS scavenging capacity methods. Cytotoxicity was reported versus Vero cells. Results. Different extracts of H. perforatum showed promising antibacterial activity against the pathogens. While the subfractions of the total extract were observed to show lesser inhibition zones and higher MIC values than the total extract of H. perforatum against MDR strains, the total extract of H. perforatum demonstrated the most potent antimicrobial action with an inhibition zone range of 17.9-27.9 mm. MDR-K. pneumoniae was discovered to be the most susceptible strain, which is consistent with the antibacterial inhibitory action of H. perforatum whole extract. Additionally, after treatment at the minimum inhibitory concentration (MIC 3.9 μg/ml), the transmission electron microscope showed alterations in the ultrastructure of the K. pneumoniae cells. Methanol extract from H. perforatum has a CC50 value of 976.75 μg/ml. Conclusion. Future inhibitors that target MDR strains may be revealed by these findings. Additionally, the extracts that were put to the test demonstrated strong antioxidant effects as shown by DPPH or ABTS radical-scavenging assays.

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Determination of Therapeutic and Safety Effects of Zygophyllum coccineum Extract in Induced Inflammation in Rats

Cited by 12 publications

References 85 publications

GC-MS Analysis, Antibacterial, and Anticancer Activities of Hibiscus sabdariffa L. Methanolic Extract: In Vitro and In Silico Studies

GC-MS Analysis, Antibacterial, and Anticancer Activities of Hibiscus sabdariffa L. Methanolic Extract: In Vitro and In Silico Studies

Estimation of the Antibacterial and Anti-Tumor Impacts of Soy Milk and Ecofriendly Myco-Manufactured Zinc Oxide Nanomaterials. <i>In vitro</i> Appraisal

In Vitro Antibacterial and Phytochemical Screening of Hypericum perforatum Extract as Potential Antimicrobial Agents against Multi‐Drug‐Resistant (MDR) Strains of Clinical Origin

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