Determination of thiamazole in serum by high-performance liquid chromatography with electrochemical detection

Tatsuhara, Tohoru; Tabuchi, Fumie; Unate, Maho; Okamura, Yukari; Shigemasa, Chiaki; Abe, Kyoju; Mashiba, Hiroto

doi:10.1016/s0378-4347(00)84636-8

Cited by 14 publications

(6 citation statements)

References 16 publications

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“…The urinary iodine level was measured by a digestion method (17). Serum MMI concentration was determined using high-performance liquid chromatography with electrochemical detection (18). The sensitivity of the MMI assay was 0.03 g/mL (263 nmol/L).…”

Section: Assay Methodsmentioning

confidence: 99%

Thyroid Function and Intellectual Development of Infants Nursed by Mothers Taking Methimazole

Azizi

Khoshniat

Bahrainian

et al. 2000

The Journal of Clinical Endocrinology & Metabolism

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For many years, breast-feeding was forbidden if antithyroid drugs were being used. Recently, limited studies have shown the relative safety of propylthiouracil and methimazole (MMI). It is not known whether MMI therapy of lactating mothers for 1 yr is safe for breast-fed infants and does not cause alterations in thyroid function and intellectual development. Between 1988 and 1998, 139 thyrotoxic lactating mothers and their infants were studied. Fifty-one thyrotoxic lactating mothers were treated with MMI during pregnancy, and MMI was continued during breast-feeding. Eighty-eight mothers were given 10 mg MMI (n 46) or 20 mg MMI (n = 42) daily for 1 month, 10 mg daily for the second month, and 5-10 mg daily thereafter. Serum T4, T3, and TSH concentrations were measured in thyrotoxic lactating mothers and their infants, before and at 1, 2, 4, 8, and 12 months. Serum MMI was measured in the infants of thyrotoxic lactating mothers taking 20 mg MMI. Thyroid function, urinary iodine, thyroid antibodies, intelligence quotient (IQ), verbal and functional components (Wechsler and Goodenough tests) were performed on 14 children of thyrotoxic lactating mothers between 48 and 74 months of age and on 17 controls. Mean +/- SD of FT4I in thyrotoxic lactating mothers treated with 10 mg MMI for 1 month decreased from 19.4 +/- 4.1 to 11.6 +/- 4.4 and from 20.5 +/- 4.7 to 9.8 +/- 1.5 when treated with 20 mg MMI. Values for FT3I decreased from 462 +/- 52 to 194 +/- 52 with 10 mg MMI and from 481 +/- 92 to 171 +/- 38 with 20 mg MMI. FT4I and FT3I were normal from the third to the twelfth months. In all infants FT4I, FT3I, and TSH concentrations were normal before and up to 12 months of MMI therapy in their lactating mothers. The lowest T4 and T3 values were 108 and 1.87 nmol/L, and the highest TSH value was 4.0 mU/L. Serum MMI levels in infants were less than 0.03 microg/mL. Six mothers receiving 20 mg MMI had increased serum TSH concentrations ranging from 26-135 mU/L after 1 month of treatment. Their infants were euthyroid with serum TSH values less than 2.6 mU/L. At 48-74 months of age, height, weight, FT4I, FT3I, TSH, and antithyroid antibody titers were not different than controls. The mean IQ was 107 +/- 14 vs. 106 +/- 16 (Goodenough test) and 103 +/- 10 vs. 103 +/- 16 (Wechsler test) for infants of thyrotoxic lactating mothers and control infants, respectively. Similarly, there was no difference in verbal and performance IQ and their components between infants of thyrotoxic lactating mothers and control children. No deleterious effects occur in thyroid function and physical and intellectual development of breast-fed infants whose lactating mothers were treated with doses of MMI up to 20 mg daily.

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Section: Assay Methodsmentioning

confidence: 99%

Thyroid Function and Intellectual Development of Infants Nursed by Mothers Taking Methimazole

Azizi

Khoshniat

Bahrainian

et al. 2000

The Journal of Clinical Endocrinology & Metabolism

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“…However, we also observed that hydrobenzole hydrochloride, thiamazole, and mizolastine had quenching effects on the TCPO-H 2 O 2 CL reaction in the presence of rhodamine 6 G; good linear curves were obtained for those three imidazole derivatives. Few methods Tatsuhara, Tabuchi, et al 1985), resonance light-scattering spectroscopy (Liu, Yuan, et al 2004), titration (Editorial Board of China Pharmacopoeia 2005), and mizolastine-only HPLC (Ding, Zhong, et al 2007). We proposed a rapid, selective, and sensitive method for their determination, and good results were obtained when it was applied to the analysis of pharmaceutical preparations.…”

Section: Introductionmentioning

confidence: 99%

Determination of Three Imidazole Derivatives by Flow-Injection Chemiluminescence

Sun

Tang

et al. 2009

Analytical Letters

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A rapid, simple, and sensitive method was developed for the determination of three imidazole derivatives based on their quenching effect on bis(2,4,6-tricholorophenyl) oxalate (TCPO)-H 2 O 2 chemiluminescence (CL) in the presence of rhodamine 6 G. Conditions affecting CL intensity were studied. With sodium dodecyl sulfate (SDS) as the additional agent, the relative standard deviation (RSD) was more twice the RSD without SDS. Under optimal conditions, good linear ranges were obtained from 1.0 Â 10 À4 g=mL to 1.0 Â 10 À6 g=mL, 1.0 Â 10 À5 g=mL to 1.0 Â 10 À7 g=mL, and 1.0 Â 10 À5 g=mL to 7.0 Â 10 À7 g=mL, with detection limits of 8.0 Â 10 À7 g=mL, 7.0 Â 10 À8 g=mL, and 8.0 Â 10 À8 g=mL (S=N ¼ 3) for hydrobenzole hydrochloride, thiamazole, and mizolastine, respectively. The RSDs for 13 consecutive injections of 1.0 Â 10 À6 g=mL hydrobenzole hydrochloride, thiamazole, and mizolastine were 1.89%, 1.47%, and 1.69%, respectively, and satisfied results were obtained with the method applied to their pharmaceutical preparations. The possible CL mechanism was simply discussed.

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“…Methimazole detection and determination in urine [3][4][5][6][7], plasma [6,[8][9][10], serum [11][12][13] and tissues [4,[13][14][15][16][17][18] have already been evaluated using various separation methods including gas chromatography-mass spectrometry [5,6], high-performance * Tel. : +48 42 6355808; fax: +48 42 6355808.…”

Section: Introductionmentioning

confidence: 99%

“…liquid chromatography-mass spectrometry [4,5,14], highperformance liquid chromatography with ultraviolet [3,[7][8][9][10]13,15] and electrochemical [12] detection, capillary zone electrophoresis with amperometric detection [11] and thin-layer chromatography [6,14,[16][17][18]. In many cases the selective isolation of methimazole and the preconcentration steps such as extraction [7,8,10] have included solid-phase extraction (SPE) [4,13,15,17], derivatization [3] or both steps [5,6,9,14,16,18] to obtain successful results.…”

Section: Introductionmentioning

confidence: 99%

Determination of methimazole in urine with the iodine-azide detection system following its separation by reversed-phase high-performance liquid chromatography

Zakrzewski

2008

Journal of Chromatography B

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Determination of thiamazole in serum by high-performance liquid chromatography with electrochemical detection

Cited by 14 publications

References 16 publications

Thyroid Function and Intellectual Development of Infants Nursed by Mothers Taking Methimazole

Thyroid Function and Intellectual Development of Infants Nursed by Mothers Taking Methimazole

Determination of Three Imidazole Derivatives by Flow-Injection Chemiluminescence

Determination of methimazole in urine with the iodine-azide detection system following its separation by reversed-phase high-performance liquid chromatography

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