Determination of Thiocyanate in Plasma and Saliva without Deproteinisation and Its Validation as a Smoking Parameter

Degiampietro, P; Peheim, E.; Drew, David A.; Graf, Hermann; Colombo, J. P.

doi:10.1515/cclm.1987.25.10.711

Cited by 24 publications

(25 citation statements)

References 12 publications

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“…These findings were similar to a study of male Chinese smokers (Chu et al, 2009). Tobacco smoke contains high concentrations of hydrogen cyanide gas, which is primarily metabolized to thiocyanate, and serum thiocyanate was potentially a better biochemical index of smoking status (Degiampietro et al, 1987). The L allele of 5-HTTLPR likely expresses a higher number of 5-HTT sites on the serotonergic synapses (Heils et al, 1997), and this could result in higher serotonin reuptake activity, which could lead to decreased serotonin availability in the synaptic cleft.…”

Section: Discussionmentioning

confidence: 99%

“…Glucose, total cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-C), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were measured using enzymatic methods by DADE Dimension ® AR. Serum thiocyanate concentrations were assessed using the colorimetric method (Degiampietro et al, 1987). Low-density lipoprotein cholesterol (LDL-C) was estimated by the Friedewald formula, as follows:…”

Section: Biochemical Measurementsmentioning

confidence: 99%

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Association of HTTLPR and 5-HT2A T102C polymorphisms with smoking characteristics and anthropometric profiles of Thai males

Suriyaprom¹,

Phonrat²,

Chuensumran³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Nicotine increases serotonin release in the brain. Gene polymorphisms in the serotonergic system have been suggested to be associated with smoking behavior. We investigated a possible association between two polymorphisms in the serotonergic system -HTTLPR of a serotonin transporter gene and 5-HT 2A at position T102C -with biochemical and anthropometric parameters, and with cigarette smoking in an investigation of 200 smokers and 111 non-smokers. The two polymorphisms, HTTLPR and 5-HT 2A at position T102C, were genotyped by PCR-RFLP. They were not significantly associated with smoking status in these Thai males. Among the smokers, thiocyanate concentrations and quantity of cigarettes smoked (cigarette pack-years) were significantly higher for individuals with LL/LS genotypes than SS HTTLPR and 5-HT 2A T102C polymorphisms and smoking genotypes of 5-HTTLPR (all P < 0.05), whereas "age at starting smoking" and "duration of smoking" were not significantly different between these two genotypes. Moreover, anthropometric variables, comprising triceps skinfold thickness, arm circumference, waist circumference, hip circumference, and waist-to-hip ratio, were significantly higher for the CC/TC genotypes of 5-HT 2A than the TT genotype (all P < 0.05), except for body mass index. HTTLPR and 5-HT 2A T102C polymorphisms were not significantly associated with smoking status among Thai males; however, the HTTLPR polymorphism among smokers appears to be an indicator of increased smoking intensity consisting of cigarette packyears and thiocyanate concentrations. The 5-HT 2A T102C polymorphism plays a role in the anthropometric profiles, triceps skinfold thickness, arm circumference, waist circumference, hip circumference, and waist-to-hip ratio, but not smoking status in Thai subjects.

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Section: Discussionmentioning

confidence: 99%

Section: Biochemical Measurementsmentioning

confidence: 99%

Association of HTTLPR and 5-HT2A T102C polymorphisms with smoking characteristics and anthropometric profiles of Thai males

Suriyaprom¹,

Phonrat²,

Chuensumran³

et al. 2012

Genet. Mol. Res.

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“…were substantiated by measurement of thiocyanate in maternal and cord serum by a manual method (33 (14). Iodine in urine and breast milk was measured by the colorimetric method of the Sandell-Kolthoff reaction after alkaline ashing, as described previously (34).…”

Section: Mothers Newbornsmentioning

confidence: 99%

Thyroglobulin in smoking mothers and their newborns at delivery suggests autoregulation of placental iodide transport overcoming thiocyanate inhibition

Andersen

Nøhr

et al. 2013

European Journal of Endocrinology

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Background: Placental transport of iodide is required for fetal thyroid hormone production. The sodium iodide symporter (NIS) mediates active iodide transport into the thyroid and the lactating mammary gland and is also present in placenta. NIS is competitively inhibited by thiocyanate from maternal smoking, but compensatory autoregulation of iodide transport differs between organs. The extent of autoregulation of placental iodide transport remains to be clarified. Objective: To compare the impact of maternal smoking on thyroglobulin (Tg) levels in maternal serum at delivery and in cord serum as markers of maternal and fetal iodine deficiency. Methods: One hundred and forty healthy, pregnant women admitted for delivery and their newborns were studied before the iodine fortification of salt in Denmark. Cotinine in urine and serum classified mothers as smokers (nZ50) or nonsmokers (nZ90). The pregnant women reported on intake of iodine-containing supplements during pregnancy and Tg in maternal serum at delivery and in cord serum were analyzed. Results: In a context of mild-to-moderate iodine deficiency, smoking mothers had significantly higher serum Tg than nonsmoking mothers (mean Tg smokers 40.2 vs nonsmokers 24.4 mg/l, PZ0.004) and so had their respective newborns (cord Tg 80.2 vs 52.4 mg/l, PZ0.006), but the ratio between Tg in cord serum and maternal serum was not significantly different in smokers compared with nonsmokers (smoking 2.06 vs nonsmoking 2.22, PZ0.69). Conclusion: Maternal smoking increased the degree of iodine deficiency in parallel in the mother and the fetus, as reflected by increased Tg levels. However, placental iodide transport seemed unaffected despite high thiocyanate levels, suggesting that thiocyanate-insensitive iodide transporters alternative to NIS are active or that NIS in the placenta is autoregulated to keep iodide transport unaltered.

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“…WBC, RBC, and platelet counts and hemoglobin and hematocrit concentrations were also determined (Coulter Counter). Serum thiocyanate concentrations were assessed using the colorimetric method (Degiampietro et al, 1987). Serum BDNF levels were determined using an enzyme-linked immunosorbent assay protocol according to the manufacturer instructions (DBD00; R & D Systems).…”

Section: Biochemical Measurementsmentioning

confidence: 99%

BDNF Val66Met polymorphism and serum concentrations of BDNF with smoking in Thai males

Suriyaprom¹,

Tungtrongchitr²,

Thawnashom³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Many studies have suggested that brain-derived neurotrophic factor (BDNF) is involved in the reward system of addiction, and that nicotine may induce alterations in BDNF gene expression and its protein level within the mesocorticolimbic system. We investigated the BDNF levels and biochemical-hematological parameters of smoker and non-smoker groups, and examined the association of the Val66Met BDNF gene polymorphism with BDNF serum levels and cigarette smoking. The study sample comprised 311 Thai volunteers (200 smokers; 111 non-smokers). The levels of serum BDNF and biochemical-hematological parameters were determined. The Val66Met BDNF polymorphism was genotyped by the polymerase chain reaction-restriction fragment length polymorphism technique. The smoker group had significantly higher serum BDNF levels than the non-smoker group (8.3 vs 6.5 ng/mL, P < 0.05). BDNF concentrations correlated positively with hemoglobin, hematocrit, red blood cell count, white blood cell count, platelet count, number of cigarettes smoked, and thiocyanate level (P < 0.05). No association was found between the BDNF Val66Met genotype and the serum BDNF level. The BDNF Val66Met polymorphism was not significantly associated with the smoking status of the Thai males in this study. Cigarette smoking may be one factor that determines the serum BDNF level, but the BDNF Val66Met polymorphism probably does not influence susceptibility to smoking among Thai males.

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Determination of Thiocyanate in Plasma and Saliva without Deproteinisation and Its Validation as a Smoking Parameter

Cited by 24 publications

References 12 publications

Association of HTTLPR and 5-HT2A T102C polymorphisms with smoking characteristics and anthropometric profiles of Thai males

Association of HTTLPR and 5-HT2A T102C polymorphisms with smoking characteristics and anthropometric profiles of Thai males

Thyroglobulin in smoking mothers and their newborns at delivery suggests autoregulation of placental iodide transport overcoming thiocyanate inhibition

BDNF Val66Met polymorphism and serum concentrations of BDNF with smoking in Thai males

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